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Although progress towards understanding of molecular pathogenesis in muscle and heart has been made, the pathways that affect the brain in DM1 is fundamentally unknown. In addition, the congenital DM1 manifest even more complicated brain abnormalities. Despite the wealth of existing cellular and animal models, iPSCs based studies are being fostered as they replicate the human model more closely to the disease. In view of this context, we set out to characterize the differentiation potential of congenital DM1 patient derived iPSC lines towards neuronal cells. Using neurogenin2 (NGN2) induced direct reprogramming of iPSCs into neurons and chemically defined media-induced neural induction protocol, we find that congenital DM1 mutant iPSC derived neurons exhibited precocious differentiation, as evidenced by their expression of pan-neuronal markers TUJ1 and Map2, along with increased processes extension and neurite length. Moreover, unbiased RNA sequencing analyses and qPCR validation revealed precocious and enhanced expression of several neurogenic transcription factors including, Ascl1, NeuroG2, and NeuroD1. Furthermore, immunofluorescence imaging of MBNL1 and MBNL2, RNA-splicing factors, displayed enhanced nuclear aggregations, a hallmark of the DM1 disease, in the mutant lines. Moreover, investigation of RNA splicing events identified mis-splicing in many important genes\/transcripts including RMST, ANK3 and MBD1 during the neural conversion of congenital DM1 lines. These studies reveal novel paradigms that may contribute to neurological pathogenesis in CDM1 patients. These studies also provide a strong foundation for future mechanistic investigation aimed at understanding CDM1 pathology and may open new avenues for the development of gene therapy approaches for individuals with DM1.\n                <\/jats:p>","DOI":"10.64898\/2026.06.25.734569","type":"posted-content","created":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T20:12:21Z","timestamp":1782850341000},"source":"Crossref","is-referenced-by-count":0,"title":["Aberrant neuronal differentiation and splicing defects in Congenital Myotonic Dystrophy (DM1) iPSC models"],"prefix":"10.64898","author":[{"given":"Surya Chandra Rao","family":"Thumu","sequence":"first","affiliation":[{"name":"Department of Cellular, Molecular and Genetic Medicine, Virginia commonwealth University School of Medicine, Richmond, Virginia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean Patrick","family":"Gonzales","sequence":"additional","affiliation":[{"name":"Department of Cellular, Molecular and Genetic Medicine, Virginia commonwealth University School of Medicine, Richmond, Virginia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soha","family":"Munir","sequence":"additional","affiliation":[{"name":"Department of Cellular, Molecular and Genetic Medicine, Virginia commonwealth University School of Medicine, Richmond, Virginia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Connor","family":"Tuck","sequence":"additional","affiliation":[{"name":"Department of Cellular, Molecular and Genetic Medicine, Virginia commonwealth University School of Medicine, Richmond, Virginia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oscar","family":"Dominguez","sequence":"additional","affiliation":[{"name":"Department of Cellular, Molecular and Genetic Medicine, Virginia commonwealth University School of Medicine, Richmond, Virginia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8069-6452","authenticated-orcid":false,"given":"Sandeep K.","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Cellular, Molecular and Genetic Medicine, Virginia commonwealth University School of Medicine, Richmond, Virginia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026070308251236000_2026.06.25.734569v2.1","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1038\/s41420-024-02074-4","article-title":"Deletion in RMST lncRNA impairs hypothalamic neuronal development in a human stem cell-based model of Kallmann Syndrome","volume":"10","year":"2024","journal-title":"Cell Death Discov"},{"key":"2026070308251236000_2026.06.25.734569v2.2","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1016\/j.nmd.2017.12.006","article-title":"Childhood-onset form of myotonic dystrophy type 1 and autism spectrum disorder: Is there comorbidity?","volume":"28","year":"2018","journal-title":"Neuromuscul Disord"},{"key":"2026070308251236000_2026.06.25.734569v2.3","doi-asserted-by":"publisher","DOI":"10.1038\/s41593-019-0399-y"},{"key":"2026070308251236000_2026.06.25.734569v2.4","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2014.08.027"},{"key":"2026070308251236000_2026.06.25.734569v2.5","doi-asserted-by":"crossref","unstructured":"Bina P , Hines MA , Sanyal J , Taneyhill LA . 2023. 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