{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T09:56:13Z","timestamp":1793354173930,"version":"4.5.0"},"posted":{"date-parts":[[2026,7,1]]},"group-title":"Immunology","reference-count":66,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Panattoni Family"},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R01CA270790"],"award-info":[{"award-number":["R01CA270790"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R01CA303968"],"award-info":[{"award-number":["R01CA303968"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"award":["DGE 2146752"],"award-info":[{"award-number":["DGE 2146752"]}],"id":[{"id":"https:\/\/ror.org\/021nxhr62","id-type":"ROR","asserted-by":"publisher"}]},{"award":["EDUC4-12790"],"award-info":[{"award-number":["EDUC4-12790"]}],"id":[{"id":"https:\/\/ror.org\/033m8b439","id-type":"ROR","asserted-by":"publisher"}]},{"award":["CRI3984"],"award-info":[{"award-number":["CRI3984"]}],"id":[{"id":"https:\/\/ror.org\/02f3xk561","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,7,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>\n                  Therapies to activate the STING immune response pathway represent promising potential anticancer treatments. However, the native STING activating molecule, 2\u2019,3\u2019-cGAMP, is a poor drug candidate due to its susceptibility to nuclease degradation and its relatively poor cell uptake. In this study, we present a nanoscale delivery vehicle based on the bacteriophage MS2 virus-like particle that can both protect cGAMP and deliver it into cells to access and bind cytosolic STING. MS2-delivered cGAMP achieved greatly increased STING activation potency relative to both free cGAMP and a nuclease-resistant synthetic cGAMP analog. In an\n                  <jats:italic>in vivo<\/jats:italic>\n                  murine colon carcinoma model, MS2-cGAMP elicited significant and prolonged antitumor activity in a STING-dependent manner at 50-fold lower concentrations relative to free cGAMP and synthetic analogs. These results demonstrate that MS2 delivery of cGAMP can yield a highly potent STING agonist immunotherapy with\n                  <jats:italic>in vivo<\/jats:italic>\n                  anticancer activity.\n                <\/jats:p>","DOI":"10.64898\/2026.06.26.734859","type":"posted-content","created":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T19:30:19Z","timestamp":1782934219000},"source":"Crossref","is-referenced-by-count":2,"title":["Viral capsid delivery of cGAMP enhances STING-dependent antitumor immune response"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3520-0032","authenticated-orcid":false,"given":"Paul","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Chemistry, University of California, Berkeley, California 94720, United 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