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Here, we use a neuron-astrocyte coculture system that promotes stellate astrocyte morphology to investigate the trafficking of late endosomes and lysosomes (LELs) in astrocytes. As astrocyte branches mature, degradative activity becomes concentrated in the soma and LELs in branches undergo bidirectional motility that becomes progressively attenuated. We establish that the lysosomal cation channel TRPML1 drives LEL immobilization. TRPML1-mediated arrest involves myosin-Va tethering to the actin cytoskeleton, which may position LELs near peripheral astrocyte processes (PAPs), fine actin-enriched protrusions that can contact synapses. Strikingly, TRPML1 activity modulates the phosphorylation of ezrin, radixin, and moesin, actin-membrane linkers enriched in PAPs. These effects are rapid and transient, suggesting a role for lysosomal TRPML1 in regulating PAP membrane dynamics. Thus, TRPML1 positions LELs proximal to PAPs which may influence PAP structural plasticity and astrocyte-synapse contacts.<\/jats:p>","DOI":"10.64898\/2026.06.26.734916","type":"posted-content","created":{"date-parts":[[2026,6,28]],"date-time":"2026-06-28T09:35:13Z","timestamp":1782639313000},"source":"Crossref","is-referenced-by-count":1,"title":["TRPML1 positions lysosomes and regulates actin-membrane linkers in astrocyte processes"],"prefix":"10.64898","author":[{"given":"Maeve L.","family":"Spivey","sequence":"first","affiliation":[{"name":"Department of Neuroscience, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, 19104, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madison L.","family":"Fuller","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, 19104, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serena J.","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, 19104, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David K.","family":"Sidibe","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, 19104, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingqi","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, 19104, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janardhan","family":"Bhattarai","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, 19104, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minghong","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, 19104, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8664-4592","authenticated-orcid":false,"given":"Sandra","family":"Maday","sequence":"additional","affiliation":[{"name":"Department of Neuroscience, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, 19104, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026063005051240000_2026.06.26.734916v1.1","doi-asserted-by":"crossref","unstructured":"Kulkarni VV , Anand A , Herr JB , Miranda C , Vogel MC , Maday S . 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