{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T23:44:55Z","timestamp":1782949495521,"version":"3.54.5"},"posted":{"date-parts":[[2026,6,30]]},"reference-count":59,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:00:00Z","timestamp":1782777600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nd\/4.0\/"}],"funder":[{"name":"UM-CFAR\/SCCC Argentina Consortium for Research and Training in Virally Induced AIDS-Malignancies","award":["U54CA221208"],"award-info":[{"award-number":["U54CA221208"]}]},{"name":"Argentinean Agency of Science, Technology and Development","award":["PICT2019-0532"],"award-info":[{"award-number":["PICT2019-0532"]}]},{"name":"Argentinean Agency of Science, Technology and Development","award":["PICT2014-0205"],"award-info":[{"award-number":["PICT2014-0205"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,6,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>Kaposi sarcoma\u2013associated herpesvirus (KSHV) reprograms host cellular pathways to promote viral persistence and tumor development by supporting the survival and expansion of infected and bystander cells. Among the signaling axes implicated in this process, oncogenic activation of receptor tyrosine kinases, particularly platelet-derived growth factor receptor alpha (PDGFRA), is a well-established hallmark of Kaposi sarcoma pathogenesis. However, the impact of virus-driven cell surface glycosylation changes in PDGFRA-associated signaling remains uncertain.<\/jats:p>\n                <jats:p>Here, we identified a critical role for KSHV in reshaping the glycosylation landscape of mesenchymal stromal cells (MSCs), thereby reprogramming PDGFRA signaling. We show that KSHV infection enhances a unique glycan profile in human and mouse MSCs, enriched in branched complex N-glycans with limited \u03b1(2-6) sialylation, Transcriptomic analyses of KSHV-infected MSCs and Kaposi\u2019s Sarcoma patient samples revealed a coordinated dysregulation of pathways involved in carbohydrate metabolism, nucleotide-sugar transport, and sialic acid turnover, with a particular focus on sialic acid turnover (NPL\/NEU3), the UDP-N-acetylglucosamine transporters SLC35A3\/B4, and complex N-glycan elongation (MGAT5\/B3GNT2\/B4GALT1) as critical nodes underlying reduced \u03b1(2-6) sialylation and increased N-glycan branching.<\/jats:p>\n                <jats:p>This remodeled glycan landscape contributes to create a permissive context for galectin-1 (Gal-1) binding, which in turn enhances PDGFRA activation and downstream signaling. These findings identify an integral component of KSHV-driven mesenchymal cell reprogramming, unveiling a lectin-dependent mechanism of RTK activation in Kaposi sarcoma pathogenesis.<\/jats:p>","DOI":"10.64898\/2026.06.29.735238","type":"posted-content","created":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T10:03:04Z","timestamp":1782813784000},"source":"Crossref","is-referenced-by-count":0,"title":["Kaposi Sarcoma Herpes Virus Reprograms Mesenchymal Cell Glycosylation to control platelet-derived growth factor receptor A signaling"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0705-6827","authenticated-orcid":false,"given":"Juli\u00e1n","family":"Gambarte Tudela","sequence":"first","affiliation":[{"name":"Instituto de Histolog\u00eda y Embriolog\u00eda de Mendoza (IHEM), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Universidad Nacional de Cuyo. M5500, Mendoza, Argentina"},{"name":"UM-CFAR\/SCCC Argentina Consortium for Research and Training in Virally Induced AIDS-Malignancies, Miami, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia N.","family":"Guti\u00e9rrez","sequence":"additional","affiliation":[{"name":"Instituto de Histolog\u00eda y Embriolog\u00eda de Mendoza (IHEM), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Universidad Nacional de Cuyo. M5500, Mendoza, Argentina"},{"name":"UM-CFAR\/SCCC Argentina Consortium for Research and Training in Virally Induced AIDS-Malignancies, Miami, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.A.","family":"Montani","sequence":"additional","affiliation":[{"name":"Instituto de Fisiolog\u00eda, Biolog\u00eda Molecular y Neurociencias (IFIBYNE), CONICET-Universidad de Buenos Aires, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8540-5995","authenticated-orcid":false,"given":"Nadia","family":"Bannoud","sequence":"additional","affiliation":[{"name":"Instituto de Histolog\u00eda y Embriolog\u00eda de Mendoza (IHEM), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Universidad Nacional de Cuyo. M5500, Mendoza, Argentina"},{"name":"UM-CFAR\/SCCC Argentina Consortium for Research and Training in Virally Induced AIDS-Malignancies, Miami, USA"},{"name":"Facultad de Ciencias M\u00e9dicas, Universidad Nacional de Cuyo. M5500, Mendoza, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo A.","family":"Garc\u00eda","sequence":"additional","affiliation":[{"name":"Instituto de Histolog\u00eda y Embriolog\u00eda de Mendoza (IHEM), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Universidad Nacional de Cuyo. M5500, Mendoza, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0581-8640","authenticated-orcid":false,"given":"Karina V.","family":"Mari\u00f1o","sequence":"additional","affiliation":[{"name":"Laboratorio de Glic\u00f3mica Funcional y Molecular, Instituto de Biolog\u00eda y Medicina Experimental (IBYME), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), C1428ADN Buenos Aires, Argentina"},{"name":"Universidad Argentina de la Empresa (UADE), Instituto de Tecnolog\u00eda (INTEC), C1073 Ciudad de Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9206-2369","authenticated-orcid":false,"given":"Martin","family":"Abba","sequence":"additional","affiliation":[{"name":"UM-CFAR\/SCCC Argentina Consortium for Research and Training in Virally Induced AIDS-Malignancies, Miami, USA"},{"name":"Centro de Investigaciones Inmunol\u00f3gicas B\u00e1sicas y Aplicadas, Facultad de Ciencias M\u00e9dicas, Universidad Nacional de La Plata, La Plata, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1888-1227","authenticated-orcid":false,"given":"Juan P.","family":"Cerliani","sequence":"additional","affiliation":[{"name":"UM-CFAR\/SCCC Argentina Consortium for Research and Training in Virally Induced AIDS-Malignancies, Miami, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6842-6815","authenticated-orcid":false,"given":"Juli\u00e1n","family":"Naipauer","sequence":"additional","affiliation":[{"name":"UM-CFAR\/SCCC Argentina Consortium for Research and Training in Virally Induced AIDS-Malignancies, Miami, USA"},{"name":"Instituto de Fisiolog\u00eda, Biolog\u00eda Molecular y Neurociencias (IFIBYNE), CONICET-Universidad de Buenos Aires, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ezequiel","family":"Lacunza","sequence":"additional","affiliation":[{"name":"UM-CFAR\/SCCC Argentina Consortium for Research and Training in Virally Induced AIDS-Malignancies, Miami, USA"},{"name":"Centro de Investigaciones Inmunol\u00f3gicas B\u00e1sicas y Aplicadas, Facultad de Ciencias M\u00e9dicas, Universidad Nacional de La Plata, La Plata, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enrique A","family":"Mesri","sequence":"additional","affiliation":[{"name":"UM-CFAR\/SCCC Argentina Consortium for Research and Training in Virally Induced AIDS-Malignancies, Miami, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabirel A.","family":"Rabinovich","sequence":"additional","affiliation":[{"name":"UM-CFAR\/SCCC Argentina Consortium for Research and Training in Virally Induced AIDS-Malignancies, Miami, USA"},{"name":"Laboratorio de Glicomedicina, Instituto de Biolog\u00eda y Medicina Experimental (IBYME), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), C1428ADN Buenos Aires, Argentina"},{"name":"Laboratory of Glycoimmunology, CaixaResearch Institute, 08035, Barcelona, Spain"},{"name":"Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Buenos Aires, C1428 Ciudad de Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8623-0645","authenticated-orcid":false,"given":"Diego O.","family":"Croci","sequence":"additional","affiliation":[{"name":"Instituto de Histolog\u00eda y Embriolog\u00eda de Mendoza (IHEM), Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Universidad Nacional de Cuyo. 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