{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T09:20:55Z","timestamp":1787908855955,"version":"build-2784847793"},"posted":{"date-parts":[[2026,7,6]]},"group-title":"Bioinformatics","reference-count":92,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T00:00:00Z","timestamp":1783296000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"award":["R35GM144083"],"award-info":[{"award-number":["R35GM144083"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,7,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>Determining the structural basis of antigen recognition by antibodies and T cell receptors (TCRs) provides critical insights into effective immune targeting and can inform design of biotherapeutics and vaccines. Accurate computational modeling of antibodies and TCRs in complex with their targets poses a major challenge for predictive methods, including AlphaFold, which is generally accurate for modeling protein complexes but has shown limited success for immune recognition. In this study we assessed the performance of AlphaFold2, AlphaFold3, increased sampling protocols, and related deep learning methods for modeling antibody-protein, antibody-peptide, and TCR-peptide-major histocompatibility complex (pMHC) recognition. We show that increased sampling and AlphaFold3 generally improve performance relative to default sampling and AlphaFold2, however predictive accuracy and improvement levels varied considerably among interface classes, with antibody-peptide complexes representing a challenge despite their small antigen size. Comparing per-case success across methods showed some complementarity, indicating opportunities for increased success through model pooling approaches, for instance increasing antibody-peptide near-native success from 41% to 59%. Analysis of AlphaFold confidence scores and modeling of a noncanonical complex provided further insights into predictive performance. These results highlight considerations for predictive antibody and TCR complex modeling efforts, while revealing key distinctions among protocols, scoring, and immune complex classes.<\/jats:p>","DOI":"10.64898\/2026.07.04.736425","type":"posted-content","created":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T04:55:20Z","timestamp":1783400120000},"source":"Crossref","is-referenced-by-count":1,"title":["Benchmarking AlphaFold and related deep learning approaches for modeling antibody and TCR antigen recognition"],"prefix":"10.64898","author":[{"given":"Rui","family":"Yin","sequence":"first","affiliation":[{"name":"Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA"},{"name":"University of Maryland Institute for Bioscience and Biotechnology Research, Rockville, MD 20850, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shayana","family":"Saravanakumar","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA"},{"name":"University of Maryland Institute for Bioscience and Biotechnology Research, Rockville, MD 20850, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu Yuan","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA"},{"name":"University of Maryland Institute for Bioscience and Biotechnology Research, Rockville, MD 20850, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minjae","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA"},{"name":"University of Maryland Institute for Bioscience and Biotechnology Research, Rockville, MD 20850, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valerie","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA"},{"name":"University of Maryland Institute for Bioscience and Biotechnology Research, Rockville, MD 20850, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA"},{"name":"University of Maryland Institute for Bioscience and Biotechnology Research, Rockville, MD 20850, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melyssa","family":"Cheung","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA"},{"name":"Department of Chemistry and Biochemistry, University of Maryland, College Park, MD 20742, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathaniel","family":"Felbinger","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA"},{"name":"University of Maryland Institute for Bioscience and Biotechnology Research, Rockville, MD 20850, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sivan","family":"Kaufman","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA"},{"name":"University of Maryland Institute for Bioscience and Biotechnology Research, Rockville, MD 20850, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maya","family":"Eisenberg","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA"},{"name":"University of Maryland Institute for Bioscience and Biotechnology Research, Rockville, MD 20850, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian G.","family":"Pierce","sequence":"additional","affiliation":[{"name":"Department of Cell Biology and Molecular Genetics, University of Maryland, College Park, MD 20742, USA"},{"name":"University of Maryland Institute for Bioscience and Biotechnology Research, Rockville, MD 20850, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026070906201885000_2026.07.04.736425v1.1","unstructured":"Janeway, C. 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