{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T10:18:50Z","timestamp":1783678730327,"version":"3.55.0"},"posted":{"date-parts":[[2026,7,7]]},"group-title":"Microbiology","reference-count":60,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T00:00:00Z","timestamp":1783382400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Foundation for Medical Research, FR","award":["FRM-EQU202103012587"],"award-info":[{"award-number":["FRM-EQU202103012587"]}]},{"DOI":"10.13039\/501100001665","name":"French National Research Agency","doi-asserted-by":"crossref","award":["ANR-18-CE35-0008"],"award-info":[{"award-number":["ANR-18-CE35-0008"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001665","name":"French National Research Agency","doi-asserted-by":"crossref","award":["ANR-19-CE11-0012"],"award-info":[{"award-number":["ANR-19-CE11-0012"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001665","name":"French National Research Agency","doi-asserted-by":"crossref","award":["ANR-23-CE12-0037"],"award-info":[{"award-number":["ANR-23-CE12-0037"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001665","name":"French National Research Agency","doi-asserted-by":"crossref","award":["ANR-10-INSB-05-02"],"award-info":[{"award-number":["ANR-10-INSB-05-02"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001665","name":"French National Research Agency","doi-asserted-by":"crossref","award":["ANR-17-EURE-0003"],"award-info":[{"award-number":["ANR-17-EURE-0003"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["1R35GM150871-01"],"award-info":[{"award-number":["1R35GM150871-01"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,7,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>Conjugative plasmids are major drivers of antibiotic resistance dissemination, yet how newly transferred plasmids establish in recipient cells remains poorly understood. Here we investigate YfjB, a previously uncharacterized conserved leading-region protein, which is zygotically induced immediately after plasmid entry and acts specifically during the earliest post-transfer stages. Multi-omics analyses reveal that YfjB reprograms host transcription, triggering extensive metabolic rewiring that compensates the transient fitness cost of plasmid acquisition. Structural analyses show that YfjB is a ParB-like protein containing a CTP-binding domain and a helix-turn-helix DNA-binding motif, linked to a previously uncharacterized dimerization module that forms a V-shaped clamp-like architecture compatible with DNA loading. Consistently, live-cell imaging reveals nucleoid-associated foci in transconjugants, and ChIP-seq identifies multiple chromosomal binding sites. We therefore rename the protein HerB (Host Expression Reprogrammer, ParB-like). More broadly, our findings reveal how mobile genetic elements facilitate their dissemination by transiently subverting host physiology.<\/jats:p>","DOI":"10.64898\/2026.07.07.736705","type":"posted-content","created":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T08:10:14Z","timestamp":1783498214000},"source":"Crossref","is-referenced-by-count":0,"title":["Plasmid-encoded host reprogramming promotes plasmid dissemination"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5791-8064","authenticated-orcid":false,"given":"Chlo\u00e9","family":"Virolle","sequence":"first","affiliation":[{"name":"Molecular Microbiology and Structural Biochemistry (MMSB), Universit\u00e9 Lyon 1, CNRS, Inserm, UMR5086, 69007 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-0190-1934","authenticated-orcid":false,"given":"Sibylle","family":"Ferrarin","sequence":"additional","affiliation":[{"name":"Molecular Microbiology and Structural Biochemistry (MMSB), Universit\u00e9 Lyon 1, CNRS, Inserm, UMR5086, 69007 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6926-6224","authenticated-orcid":false,"given":"Ga\u00ebl","family":"Panis","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Medicine, Faculty of Medicine, University of Geneva, Geneva, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yannick","family":"Baffert","sequence":"additional","affiliation":[{"name":"Molecular Microbiology and Structural Biochemistry (MMSB), Universit\u00e9 Lyon 1, CNRS, Inserm, UMR5086, 69007 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9982-8793","authenticated-orcid":false,"given":"Annick","family":"Dedieu-Berne","sequence":"additional","affiliation":[{"name":"Molecular Microbiology and Structural Biochemistry (MMSB), Universit\u00e9 Lyon 1, CNRS, Inserm, UMR5086, 69007 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9r\u00e9my","family":"Gu\u00e9rin","sequence":"additional","affiliation":[{"name":"Molecular Microbiology and Structural Biochemistry (MMSB), Universit\u00e9 Lyon 1, CNRS, Inserm, UMR5086, 69007 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6607-311X","authenticated-orcid":false,"given":"Julien","family":"Cayron","sequence":"additional","affiliation":[{"name":"Molecular Microbiology and Structural Biochemistry (MMSB), Universit\u00e9 Lyon 1, CNRS, Inserm, UMR5086, 69007 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1001-4716","authenticated-orcid":false,"given":"Daouda A K","family":"Traor\u00e9","sequence":"additional","affiliation":[{"name":"Facult\u00e9 des Sciences et Techniques, Universit\u00e9 des Science Techniques et Technologiques de Bamako (USTTB), Bamako, Mali"},{"name":"Faculty of Natural Sciences, School of Life Sciences, Keele University, Staffordshire ST5 5BG, United Kingdom"},{"name":"Life Science Group, Institute Laue-Langevin, 38042 Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3183-5468","authenticated-orcid":false,"given":"Sof\u00eda","family":"Mart\u00ednez-Absal\u00f3n","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, University of Rochester, Rochester, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rania","family":"Zenati","sequence":"additional","affiliation":[{"name":"Molecular Microbiology and Structural Biochemistry (MMSB), Universit\u00e9 Lyon 1, CNRS, Inserm, UMR5086, 69007 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0320-0764","authenticated-orcid":false,"given":"Fr\u00e9d\u00e9ric","family":"Delolme","sequence":"additional","affiliation":[{"name":"Protein Science Facility, SFR BioScience, CNRS, University Lyon 1, ENS, 69007 Lyon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7885-1014","authenticated-orcid":false,"given":"Adeline","family":"Page","sequence":"additional","affiliation":[{"name":"Protein Science Facility, SFR BioScience, CNRS, University Lyon 1, ENS, 69007 Lyon"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9340-6303","authenticated-orcid":false,"given":"Sarah","family":"Bigot","sequence":"additional","affiliation":[{"name":"Molecular Microbiology and Structural Biochemistry (MMSB), Universit\u00e9 Lyon 1, CNRS, Inserm, UMR5086, 69007 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0835-5407","authenticated-orcid":false,"given":"Yoshiharu","family":"Yamaichi","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), 91198 Gif-sur-Yvette, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0018-9205","authenticated-orcid":false,"given":"Allison","family":"Lopatkin","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, University of Rochester, Rochester, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5249-9910","authenticated-orcid":false,"given":"Patrick H.","family":"Viollier","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Medicine, Faculty of Medicine, University of Geneva, Geneva, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6219-1880","authenticated-orcid":false,"given":"David","family":"Burstein","sequence":"additional","affiliation":[{"name":"The Shmunis School of Biomedicine and Cancer Research, George S. Wise Faculty of Life Sciences, Tel-Aviv University, Tel-Aviv, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4279-9167","authenticated-orcid":false,"given":"Laurent","family":"Terradot","sequence":"additional","affiliation":[{"name":"Molecular Microbiology and Structural Biochemistry (MMSB), Universit\u00e9 Lyon 1, CNRS, Inserm, UMR5086, 69007 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9108-0848","authenticated-orcid":false,"given":"Christian","family":"Lesterlin","sequence":"additional","affiliation":[{"name":"Molecular Microbiology and Structural Biochemistry (MMSB), Universit\u00e9 Lyon 1, CNRS, Inserm, UMR5086, 69007 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026071003051168000_2026.07.07.736705v1.1","doi-asserted-by":"crossref","unstructured":"Couturier, A. et al. Real-time visualisation of the intracellular dynamics of conjugative plasmid transfer. Nat Commun 14, 294 (2023).","DOI":"10.1038\/s41467-023-35978-3"},{"key":"2026071003051168000_2026.07.07.736705v1.2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaf1299"},{"key":"2026071003051168000_2026.07.07.736705v1.3","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80275-5"},{"key":"2026071003051168000_2026.07.07.736705v1.4","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07994-w"},{"key":"2026071003051168000_2026.07.07.736705v1.5","doi-asserted-by":"crossref","unstructured":"Fraikin, N. , Samuel, B. , Burstein, D. & Lesterlin, C . Strategies for zygotic gene expression during plasmid establishment. Plasmid 134, 102754 (2025).","DOI":"10.1016\/j.plasmid.2025.102754"},{"key":"2026071003051168000_2026.07.07.736705v1.6","doi-asserted-by":"crossref","first-page":"12858","DOI":"10.1093\/nar\/gkaa1163","article-title":"Plasmid replication-associated single-strand-specific methyltransferases","volume":"48","year":"2020","journal-title":"Nucleic Acids Res"},{"key":"2026071003051168000_2026.07.07.736705v1.7","doi-asserted-by":"publisher","DOI":"10.64898\/2026.01.09.698686"},{"key":"2026071003051168000_2026.07.07.736705v1.8","doi-asserted-by":"crossref","first-page":"e1004766","DOI":"10.1371\/journal.pgen.1004766","article-title":"Plasmid Flux in Escherichia coli ST131 Sublineages, Analyzed by Plasmid Constellation Network (PLACNET), a New Method for Plasmid Reconstruction from Whole Genome Sequences","volume":"10","year":"2014","journal-title":"PLOS Genetics"},{"key":"2026071003051168000_2026.07.07.736705v1.9","doi-asserted-by":"crossref","unstructured":"Fernandez-Lopez, R. , de Toro, M. , Moncalian, G. , Garcillan-Barcia, M. P. & de la Cruz, F . Comparative Genomics of the Conjugation Region of F-like Plasmids: Five Shades of F. Front. Mol. Biosci. 3, (2016).","DOI":"10.3389\/fmolb.2016.00071"},{"key":"2026071003051168000_2026.07.07.736705v1.10","first-page":"e00121","article-title":"Separate F-Type Plasmids Have Shaped the Evolution of the H30 Subclone of Escherichia coli Sequence Type 131","volume":"1","year":"2016","journal-title":"mSphere"},{"key":"2026071003051168000_2026.07.07.736705v1.11","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2004.06.002"},{"key":"2026071003051168000_2026.07.07.736705v1.12","doi-asserted-by":"crossref","first-page":"e1006908","DOI":"10.1371\/journal.pgen.1006908","article-title":"The nucleoid occlusion factor Noc controls DNA replication initiation in Staphylococcus aureus","volume":"13","year":"2017","journal-title":"PLOS Genetics"},{"key":"2026071003051168000_2026.07.07.736705v1.13","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-02015-z"},{"key":"2026071003051168000_2026.07.07.736705v1.14","doi-asserted-by":"crossref","first-page":"gkaf802","DOI":"10.1093\/nar\/gkaf802","article-title":"Orchestrated long-distance gene activation by a ParB-like BisD-CTP DNA clamp in low-frequency transfer competence development in Pseudomonas putida","volume":"53","year":"2025","journal-title":"Nucleic Acids Res"},{"key":"2026071003051168000_2026.07.07.736705v1.15","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms6322"},{"key":"2026071003051168000_2026.07.07.736705v1.16","doi-asserted-by":"publisher","DOI":"10.1126\/science.aav6390"},{"key":"2026071003051168000_2026.07.07.736705v1.17","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.04.006"},{"key":"2026071003051168000_2026.07.07.736705v1.18","doi-asserted-by":"publisher","DOI":"10.1038\/nature12868"},{"key":"2026071003051168000_2026.07.07.736705v1.19","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2021.01.039"},{"key":"2026071003051168000_2026.07.07.736705v1.20","doi-asserted-by":"publisher","DOI":"10.1038\/S41586-024-07487-W"},{"key":"2026071003051168000_2026.07.07.736705v1.21","doi-asserted-by":"publisher","DOI":"10.1093\/femsre\/fuad067"},{"key":"2026071003051168000_2026.07.07.736705v1.22","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-47094-x"},{"key":"2026071003051168000_2026.07.07.736705v1.23","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2009.120"},{"key":"2026071003051168000_2026.07.07.736705v1.24","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-25429-2"},{"key":"2026071003051168000_2026.07.07.736705v1.25","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.69676"},{"key":"2026071003051168000_2026.07.07.736705v1.26","doi-asserted-by":"crossref","unstructured":"Funnell, B. E . ParB Partition Proteins: Complex Formation and Spreading at Bacterial and Plasmid Centromeres. Front Mol Biosci 3, 44 (2016).","DOI":"10.3389\/fmolb.2016.00044"},{"key":"2026071003051168000_2026.07.07.736705v1.27","doi-asserted-by":"publisher","DOI":"10.1101\/gad.242206.114"},{"key":"2026071003051168000_2026.07.07.736705v1.28","doi-asserted-by":"publisher","DOI":"10.1126\/science.aay3965"},{"key":"2026071003051168000_2026.07.07.736705v1.29","doi-asserted-by":"publisher","DOI":"10.1101\/2021.05.05.442810"},{"key":"2026071003051168000_2026.07.07.736705v1.30","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-38022-6"},{"key":"2026071003051168000_2026.07.07.736705v1.31","doi-asserted-by":"publisher","DOI":"10.15252\/msb.20209913"},{"key":"2026071003051168000_2026.07.07.736705v1.32","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab1111"},{"key":"2026071003051168000_2026.07.07.736705v1.33","first-page":"214","article-title":"INfrastructure for a PHAge REference Database: Identification of Large-Scale Biases in the Current Collection of Cultured Phage Genomes","volume":"2","year":"2021","journal-title":"PHAGE"},{"key":"2026071003051168000_2026.07.07.736705v1.34","doi-asserted-by":"publisher","DOI":"10.1038\/nature15765"},{"key":"2026071003051168000_2026.07.07.736705v1.35","doi-asserted-by":"crossref","first-page":"e0119325","DOI":"10.1128\/mbio.01193-25","article-title":"mGem: Revisiting bacterial overflow metabolism","volume":"16","year":"2025","journal-title":"mBio"},{"key":"2026071003051168000_2026.07.07.736705v1.36","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1004913"},{"key":"2026071003051168000_2026.07.07.736705v1.37","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.12.3768-3776.1999"},{"key":"2026071003051168000_2026.07.07.736705v1.38","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-genet-120215-035130"},{"key":"2026071003051168000_2026.07.07.736705v1.39","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2527592122"},{"key":"2026071003051168000_2026.07.07.736705v1.40","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.120163297"},{"key":"2026071003051168000_2026.07.07.736705v1.41","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.100127597"},{"key":"2026071003051168000_2026.07.07.736705v1.42","doi-asserted-by":"publisher","DOI":"10.3791\/60576"},{"key":"2026071003051168000_2026.07.07.736705v1.43","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1007\/978-1-4939-3631-1_6","article-title":"Investigating Bacterial Chromosome Architecture","volume":"1431","year":"2016","journal-title":"Methods Mol. Biol"},{"key":"2026071003051168000_2026.07.07.736705v1.44","doi-asserted-by":"crossref","unstructured":"Ducret, A. , Quardokus, E. M. & Brun, Y. V . MicrobeJ, a tool for high throughput bacterial cell detection and quantitative analysis. Nat Microbiol 1, 16077 (2016).","DOI":"10.1038\/nmicrobiol.2016.77"},{"key":"2026071003051168000_2026.07.07.736705v1.45","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4169"},{"key":"2026071003051168000_2026.07.07.736705v1.46","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2015.08.008"},{"key":"2026071003051168000_2026.07.07.736705v1.47","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-020-00990-8"},{"key":"2026071003051168000_2026.07.07.736705v1.48","doi-asserted-by":"publisher","DOI":"10.1002\/PRO.3943"},{"key":"2026071003051168000_2026.07.07.736705v1.49","doi-asserted-by":"publisher","DOI":"10.1002\/pro.3865"},{"key":"2026071003051168000_2026.07.07.736705v1.50","doi-asserted-by":"crossref","first-page":"913","DOI":"10.1016\/j.str.2021.03.011","article-title":"Macromolecular refinement of X-ray and cryoelectron microscopy structures with Phenix\/OPLS3e for improved structure and ligand quality","volume":"29","year":"2021","journal-title":"Structure"},{"key":"2026071003051168000_2026.07.07.736705v1.51","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw408"},{"key":"2026071003051168000_2026.07.07.736705v1.52","first-page":"125","article-title":"Acta Crystallogr D","volume":"66","year":"2010","journal-title":"Biol Crystallogr"},{"key":"2026071003051168000_2026.07.07.736705v1.53","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444913000061"},{"key":"2026071003051168000_2026.07.07.736705v1.54","doi-asserted-by":"crossref","unstructured":"Hough, M. A. & Wilson, K. S . From crystal to structure with CCP4. Acta Crystallogr D Struct Biol 74, 67 (2018).","DOI":"10.1107\/S2059798317017557"},{"key":"2026071003051168000_2026.07.07.736705v1.55","doi-asserted-by":"crossref","unstructured":"Nolte, H. , MacVicar, T. D. , Tellkamp, F. & Kr\u00fcger, M . Instant Clue: A Software Suite for Interactive Data Visualization and Analysis. Sci Rep 8, 12648 (2018).","DOI":"10.1038\/s41598-018-31154-6"},{"key":"2026071003051168000_2026.07.07.736705v1.56","doi-asserted-by":"crossref","first-page":"e1002195","DOI":"10.1371\/journal.pcbi.1002195","article-title":"Accelerated Profile HMM Searches","volume":"7","year":"2011","journal-title":"PLOS Computational Biology"},{"key":"2026071003051168000_2026.07.07.736705v1.57","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btl158"},{"key":"2026071003051168000_2026.07.07.736705v1.58","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/mst010"},{"key":"2026071003051168000_2026.07.07.736705v1.59","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu033"},{"key":"2026071003051168000_2026.07.07.736705v1.60","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkae268"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.64898\/2026.07.07.736705","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T10:05:19Z","timestamp":1783677919000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.64898\/2026.07.07.736705"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,7]]},"references-count":60,"URL":"https:\/\/doi.org\/10.64898\/2026.07.07.736705","relation":{},"subject":[],"published":{"date-parts":[[2026,7,7]]},"subtype":"preprint"}}