{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T10:11:53Z","timestamp":1784110313255,"version":"3.55.0"},"posted":{"date-parts":[[2026,7,12]]},"group-title":"Genetics","reference-count":28,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,7,12]],"date-time":"2026-07-12T00:00:00Z","timestamp":1783814400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R01HL156499"],"award-info":[{"award-number":["R01HL156499"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,7,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>\n                    We previously performed a strain intercross between atherosclerosis resistant AKR\n                    <jats:italic>Apoe<\/jats:italic>\n                    <jats:sup>-\/-<\/jats:sup>\n                    mice and atherosclerosis sensitive DBA\/2\n                    <jats:italic>Apoe<\/jats:italic>\n                    <jats:sup>-\/-<\/jats:sup>\n                    mice and identified the\n                    <jats:italic>Ath28<\/jats:italic>\n                    quantitative trait locus (QTL) on the distal end of chromosome 2. Congenic strain fine mapping identified the\n                    <jats:italic>Ath28<\/jats:italic>\n                    .\n                    <jats:italic>1<\/jats:italic>\n                    QTL atherosclerosis modifying subregion, encompassing 217 Kb, containing for only three protein-coding genes,\n                    <jats:italic>Zbp1, Pck1<\/jats:italic>\n                    , and\n                    <jats:italic>Pmepa1<\/jats:italic>\n                    , encoding respectively, Z-DNA binding protein 1, phosphoenolpyruvate carboxykinase 1, and prostate transmembrane protein androgen induced 1.\n                  <\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>\n                    The effect of macrophage-specific knockout of\n                    <jats:italic>Pck1<\/jats:italic>\n                    (KO) was tested using the AAV2 transduced proprotein convertase subtilisin kexin type 9 (PCSK9) overexpression mouse model of hyperlipidemia and atherosclerosis.\n                  <\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>\n                    Unexpectedly, macrophage\n                    <jats:italic>Pck1<\/jats:italic>\n                    deficiency lowered body weight, liver weight, and HDL-cholesterol levels in both sexes, while total and non-HDL cholesterol levels were only decreased in male mice. Aortic root lesion area and necrotic lesion area were unchanged in KO mice of both sexes.\n                  <\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Pck1<\/jats:italic>\n                    was not confirmed as an atherosclerosis modifier gene.\n                  <\/jats:p>\n                <\/jats:sec>","DOI":"10.64898\/2026.07.08.737280","type":"posted-content","created":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T03:35:14Z","timestamp":1783913714000},"source":"Crossref","is-referenced-by-count":0,"title":["Myeloid-Specific\n                  <i>Pck1<\/i>\n                  Deficiency Does Not Alter Aortic Root Atherosclerosis in Mice"],"prefix":"10.64898","author":[{"given":"Juying","family":"Han","sequence":"first","affiliation":[{"name":"Department of Heart, Blood, and Kidney Research, Cleveland Clinic, Cleveland, OH, USA, 44195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuel","family":"Opoku","sequence":"additional","affiliation":[{"name":"Department of Heart, Blood, and Kidney Research, Cleveland Clinic, Cleveland, OH, USA, 44195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0415-386X","authenticated-orcid":false,"given":"Jonathan D.","family":"Smith","sequence":"additional","affiliation":[{"name":"Department of Heart, Blood, and Kidney Research, Cleveland Clinic, Cleveland, OH, USA, 44195"},{"name":"Department of Molecular Medicine, Cleveland Clinic Lerner College of Medicine, Case Western Reserve University School of Medicine, Cleveland, OH, USA44195"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026071503001306000_2026.07.08.737280v1.1","doi-asserted-by":"publisher","DOI":"10.1038\/s41588-018-0183-z"},{"key":"2026071503001306000_2026.07.08.737280v1.2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-60327-375-6_18"},{"key":"2026071503001306000_2026.07.08.737280v1.3","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.62047"},{"key":"2026071503001306000_2026.07.08.737280v1.4","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000047461.18902.80"},{"key":"2026071503001306000_2026.07.08.737280v1.5","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000201044.33220.5c"},{"key":"2026071503001306000_2026.07.08.737280v1.6","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.112.005421"},{"key":"2026071503001306000_2026.07.08.737280v1.7","doi-asserted-by":"crossref","first-page":"70","DOI":"10.3390\/genes13010070","article-title":"Fine Mapping of the Mouse Ath28 Locus Yields Three Atherosclerosis Modifying Sub-Regions","volume":"13","year":"2021","journal-title":"Genes (Basel)"},{"key":"2026071503001306000_2026.07.08.737280v1.8","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2025.120542"},{"key":"2026071503001306000_2026.07.08.737280v1.9","doi-asserted-by":"publisher","DOI":"10.1007\/s12013-007-0025-6"},{"key":"2026071503001306000_2026.07.08.737280v1.10","doi-asserted-by":"publisher","DOI":"10.1172\/JCI161713"},{"key":"2026071503001306000_2026.07.08.737280v1.11","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.20.17.6508-6517.2000"},{"key":"2026071503001306000_2026.07.08.737280v1.12","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M117.819136"},{"key":"2026071503001306000_2026.07.08.737280v1.13","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.114.302937"},{"key":"2026071503001306000_2026.07.08.737280v1.14","doi-asserted-by":"publisher","DOI":"10.1385\/1-59745-213-0:83"},{"key":"2026071503001306000_2026.07.08.737280v1.15","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.117.309524"},{"key":"2026071503001306000_2026.07.08.737280v1.16","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2016.06.011"},{"key":"2026071503001306000_2026.07.08.737280v1.17","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcmgh.2020.07.012"},{"key":"2026071503001306000_2026.07.08.737280v1.18","doi-asserted-by":"publisher","DOI":"10.1101\/2023.07.13.548886"},{"key":"2026071503001306000_2026.07.08.737280v1.19","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.118.038362"},{"key":"2026071503001306000_2026.07.08.737280v1.20","doi-asserted-by":"crossref","unstructured":"Getz, G.S. , and Reardon, C.A. 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