{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T18:02:52Z","timestamp":1784397772166,"version":"3.55.0"},"posted":{"date-parts":[[2026,7,16]]},"group-title":"Immunology","reference-count":40,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T00:00:00Z","timestamp":1784160000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R56-AI174413"],"award-info":[{"award-number":["R56-AI174413"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000005","name":"United States Department of Defense","doi-asserted-by":"crossref","award":["HT9425-23-1-0271, LR220036"],"award-info":[{"award-number":["HT9425-23-1-0271, LR220036"]}],"id":[{"id":"10.13039\/100000005","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,7,16]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Numbers of ARID3a (\n                  <jats:underline>AT<\/jats:underline>\n                  -\n                  <jats:underline>R<\/jats:underline>\n                  ich Interaction\n                  <jats:underline>D<\/jats:underline>\n                  omain\n                  <jats:underline>3a<\/jats:underline>\n                  ) -expressing B lymphocytes from patients with systemic lupus erythematosus (SLE) are associated with increased disease activity. Normally, ARID3a-expressing circulating na\u00efve B cells are rare, but in SLE na\u00efve B cells dramatically increase ARID3a expression. We found that\n                  <jats:italic>in vitro<\/jats:italic>\n                  stimulation of B lymphocytes from healthy individuals with a cocktail of cytokines and agonists induced ARID3a in a subset of activated na\u00efve B cells and in IgD\n                  <jats:sup>-<\/jats:sup>\n                  CD27\n                  <jats:sup>-<\/jats:sup>\n                  double negative B cells previously associated with autoimmunity. Single cell RNA-seq of isolated na\u00efve B cells from ten SLE patients, with varying frequencies of ARID3a-expressing cells, revealed that ARID3a-associated genes included activation markers. Moreover, our data revealed the unexpected co-expression of the scavenger receptor CD68 with ARID3a, at both the transcript and protein level, in activated subsets of na\u00efve B cells. Inhibition of ARID3a in stimulated B cell cultures blocked na\u00efve B cell activation and CD68 expression. Together, these data identify ARID3a and CD68 as markers of na\u00efve B cell precursors associated with autoimmunity in SLE.\n                <\/jats:p>","DOI":"10.64898\/2026.07.10.737835","type":"posted-content","created":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T04:15:16Z","timestamp":1784261716000},"source":"Crossref","is-referenced-by-count":0,"title":["ARID3a-Expressing Na\u00efve B Cells in SLE have an Activated Phenotype and Transiently Express Surface CD68"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4549-1224","authenticated-orcid":false,"given":"Joshua","family":"Garton","sequence":"first","affiliation":[{"name":"The Departments of Internal Medicine, University of Oklahoma Health Sciences Center"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7111-9119","authenticated-orcid":false,"given":"James","family":"Hocker","sequence":"additional","affiliation":[{"name":"The Departments of Internal 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