{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T14:28:35Z","timestamp":1785421715223,"version":"3.56.0"},"posted":{"date-parts":[[2026,7,16]]},"group-title":"Cell Biology","reference-count":27,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T00:00:00Z","timestamp":1784160000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"award":["3R01AR078375-05S1"],"award-info":[{"award-number":["3R01AR078375-05S1"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["T32GM008666"],"award-info":[{"award-number":["T32GM008666"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Wu Tsai Human Performance Alliance"},{"name":"Rita L. Atkinson Graduate Fellowship"},{"name":"Cheng Family Innovative Research Award"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,7,16]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Satellite Cells (SCs) and Fibro-Adipogenic Progenitors (FAPs) are muscle-resident cell populations crucial for maintaining skeletal muscle homeostasis and coordinating regeneration after injuries. However, primary human SCs and FAPs are difficult to co-isolate, and their broad use in translational research has been limited by a lack of standardized biobanking protocols. Recently, we published a protocol for efficient co-isolation of SCs and FAPs from human skeletal muscle. Here, we extend those efforts to establish a comprehensive pipeline for the cryopreservation, cold-chain transport, and independent-site utilization of human SCs and FAPs. Cells taken through this pipeline maintained lineage-specific markers, including Pax7, MyoD and CD56 for SCs, and PDGFR\u03b1 and TE7 for FAPs, indicating retention of their pre-biobanking phenotype. Furthermore, SCs demonstrate robust myogenic differentiation capacity, and FAPs demonstrate both fibrogenic and adipogenic differentiation capacity post-transport. Finally, previously biobanked SCs were incorporated into\n                  <jats:italic>in vitro<\/jats:italic>\n                  3D muscle constructs, demonstrating their utility for human-based New Approach Methodologies (NAMs). This framework for multi-site collaboration facilitates broader access to human primary muscle cells, which will improve the scalability and translatability of human-based NAMs for skeletal muscle research.\n                <\/jats:p>","DOI":"10.64898\/2026.07.15.738758","type":"posted-content","created":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T14:05:20Z","timestamp":1784210720000},"source":"Crossref","is-referenced-by-count":0,"title":["A Multi-Institution Biobanking Pipeline for Primary Human Satellite Cells and Fibro-Adipogenic Progenitors"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-7875-6489","authenticated-orcid":false,"given":"Frank S.","family":"Pittman","sequence":"first","affiliation":[{"name":"Department of Bioengineering, Phil and Penny Knight Campus for Accelerating Scientific Impact, University of Oregon; Eugene, Oregon, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8986-3659","authenticated-orcid":false,"given":"Adam","family":"Rauff","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, Phil and Penny Knight Campus for Accelerating Scientific Impact, University of Oregon; Eugene, Oregon, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5069-9882","authenticated-orcid":false,"given":"Grace E.","family":"Privett","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, Phil and Penny Knight Campus for Accelerating Scientific Impact, University of Oregon; Eugene, Oregon, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5627-1681","authenticated-orcid":false,"given":"Alis","family":"Balayan","sequence":"additional","affiliation":[{"name":"Biomedical Sciences Program, UC San Diego, La Jolla, California, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8896-635X","authenticated-orcid":false,"given":"Severin","family":"Ruoss","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery, UC San Diego, La Jolla, California, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0303-261X","authenticated-orcid":false,"given":"Robert E.","family":"Guldberg","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, Phil and Penny Knight Campus for Accelerating Scientific Impact, University of Oregon; Eugene, Oregon, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine M.","family":"Robertson","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery, UC San Diego, La Jolla, California, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1642-5380","authenticated-orcid":false,"given":"Adam J.","family":"Engler","sequence":"additional","affiliation":[{"name":"Biomedical Sciences Program, UC San Diego, La Jolla, California, United States"},{"name":"Chien-Lay Department of Bioengineering, UC San Diego, La Jolla, California, United States"},{"name":"Sanford Consortium for Regenerative Medicine, La Jolla, California, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4470-155X","authenticated-orcid":false,"given":"Samuel R.","family":"Ward","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery, UC San Diego, La Jolla, California, United States"},{"name":"Department of Radiology, UC San Diego, La Jolla, California, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3421-2549","authenticated-orcid":false,"given":"Nick J.","family":"Willett","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, Phil and Penny Knight Campus for Accelerating Scientific Impact, University of Oregon; 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