{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T11:38:19Z","timestamp":1785411499999,"version":"3.56.0"},"posted":{"date-parts":[[2026,7,16]]},"group-title":"Molecular Biology","reference-count":71,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T00:00:00Z","timestamp":1784160000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"crossref","award":["R35GM151016"],"award-info":[{"award-number":["R35GM151016"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"crossref"}]},{"award":["R00HD099316"],"award-info":[{"award-number":["R00HD099316"]}],"id":[{"id":"https:\/\/ror.org\/04byxyr05","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,7,16]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Heterochromatin and its core effectors from the Heterochromatin Protein 1 (HP1) family are essential for epigenetic silencing of repetitive regions and genome integrity from yeast to humans. However, how HP1s and associated factors regulate heterochromatin properties\n                  <jats:italic>in vivo<\/jats:italic>\n                  to sustain silencing across development, aging, and environmental factors, remains incompletely understood. Here, we identify Clump\/CG30403 \u2014 a previously uncharacterized MADF-BESS domain protein \u2014 as a novel heterochromatin factor required for robust transposon silencing during\n                  <jats:italic>Drosophila<\/jats:italic>\n                  oogenesis and sustained fertility with age and temperature stress. Clump\/CG30403 interacts with the main HP1-family silencing effector Su(var)205\/HP1a through a noncanonical binding motif within its large intrinsically disordered region. Notably, in the absence of Clump\/CG30403, HP1a mobility and silencing capacity are compromised despite largely unperturbed genomic distribution, showing that HP1a presence alone is insufficient for repression. We also show that Clump\/CG30403 uniquely accumulates at its own promoter to self-repress and prevent ectopic aggregation, revealing a feedback mechanism to constrain protein dosage and phase behavior. Overall, we propose that Clump\/CG30403 is a HP1 corepressor that acts as a tightly calibrated safeguard of the heterochromatin environment properties to ensure stable silencing, genome integrity, and persistent reproductive function.\n                <\/jats:p>","DOI":"10.64898\/2026.07.15.738798","type":"posted-content","created":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T14:05:20Z","timestamp":1784210720000},"source":"Crossref","is-referenced-by-count":0,"title":["The novel HP1a interactor Clump\/CG30403 safeguards transposon silencing and reproductive resilience in the\n                  <i>Drosophila<\/i>\n                  ovary"],"prefix":"10.64898","author":[{"given":"Kun","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Biochemistry, University of California Riverside 3401 Watkins Drive, Boyce Hall 1415A Riverside, CA 92521, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of California Riverside 3401 Watkins Drive, Boyce Hall 1415A Riverside, CA 92521, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Garcia","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of California Riverside 3401 Watkins Drive, Boyce Hall 1415A Riverside, CA 92521, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of California Riverside 3401 Watkins Drive, Boyce Hall 1415A Riverside, CA 92521, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4958-1032","authenticated-orcid":false,"given":"Jikui","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of California Riverside 3401 Watkins Drive, Boyce Hall 1415A Riverside, CA 92521, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5051-5502","authenticated-orcid":false,"given":"Maria","family":"Ninova","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of California Riverside 3401 Watkins Drive, Boyce Hall 1415A Riverside, CA 92521, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026071809450874000_2026.07.15.738798v1.1","doi-asserted-by":"publisher","DOI":"10.1038\/S41586-024-07487-W"},{"key":"2026071809450874000_2026.07.15.738798v1.2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2013.06.013"},{"key":"2026071809450874000_2026.07.15.738798v1.3","doi-asserted-by":"publisher","DOI":"10.1101\/gad.241950.114"},{"key":"2026071809450874000_2026.07.15.738798v1.4","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2017.119"},{"key":"2026071809450874000_2026.07.15.738798v1.5","doi-asserted-by":"publisher","DOI":"10.1038\/35065138"},{"key":"2026071809450874000_2026.07.15.738798v1.6","doi-asserted-by":"publisher","DOI":"10.1186\/s13100-015-0041-9"},{"key":"2026071809450874000_2026.07.15.738798v1.7","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(02)01058-2"},{"key":"2026071809450874000_2026.07.15.738798v1.8","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.01.043"},{"key":"2026071809450874000_2026.07.15.738798v1.9","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2014.01.002"},{"key":"2026071809450874000_2026.07.15.738798v1.10","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2018.10.038"},{"key":"2026071809450874000_2026.07.15.738798v1.11","doi-asserted-by":"crossref","unstructured":"Chavan A , Isenhart R , Nguyen SC , Kotb N , Harke J , Sintsova A , Ulukaya G , Uliana F , Ashiono C , Kutay U et al. 2023. 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