{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T20:30:10Z","timestamp":1793478610977,"version":"4.5.0"},"posted":{"date-parts":[[2026,7,19]]},"group-title":"Biophysics","reference-count":62,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,7,19]],"date-time":"2026-07-19T00:00:00Z","timestamp":1784419200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nd\/4.0\/"}],"funder":[{"award":["848586"],"award-info":[{"award-number":["848586"]}],"id":[{"id":"https:\/\/ror.org\/013kjyp64","id-type":"ROR","asserted-by":"publisher"}]},{"award":["R35GM147050"],"award-info":[{"award-number":["R35GM147050"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["CAREER #2439729"],"award-info":[{"award-number":["CAREER #2439729"]}],"id":[{"id":"https:\/\/ror.org\/021nxhr62","id-type":"ROR","asserted-by":"publisher"}]},{"award":["REU Site: Ole Miss Nanoengineering Summer REU Program #2148764"],"award-info":[{"award-number":["REU Site: Ole Miss Nanoengineering Summer REU Program #2148764"]}],"id":[{"id":"https:\/\/ror.org\/021nxhr62","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,10,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>Myosin II generates force through the collective action of mechanically coupled motor ensembles, yet the mechanisms by which these ensembles sense changes in motor occupancy and coordinate force generation remain poorly understood. Ensemble force production may be governed by an optimal balance between effective motor occupancy and mechanical coordination rather than by motor number alone. We reconstituted cardiac myosin ensembles and systematically perturbed effective motor occupancy using the small-molecule drugs omecamtiv mecarbil (OM), which prolongs actomyosin interactions, and mavacamten (MAVA), which reduces the number of available force-generating myosin heads. Optical trapping measurements of full-length and S1 cardiac myosin ensembles revealed that force generation depended on both myosin concentration and pharmacological perturbation. Reducing myosin concentration increased force generation in the absence of drug, while OM and MAVA produced responses that varied with the initial occupancy state of the ensemble. Low concentrations of MAVA enhanced force generation under high motor occupancy but reduced force under low motor occupancy, whereas OM produced occupancy-dependent changes in both endpoint force and force dynamics. Force traces further revealed changes in the persistence and temporal coordination of force generation. These findings support a model in which cardiac myosin ensembles operate along an occupancy\u2013coordination landscape, where maximal force generation is achieved at an intermediate level of effective motor occupancy. Our results illuminate how changes in motor occupancy are translated into coordinated ensemble mechanics and suggest that emergent mechanical feedback through the shared actin filament may enable ensembles to collectively sense and adapt to their mechanical state.<\/jats:p>\n                <jats:sec>\n                  <jats:title>Statement of Significance<\/jats:title>\n                  <jats:p>Force generation by muscle emerges from the coordinated activity of myosin ensembles, yet the principles governing this collective behavior remain poorly understood. Using an in vitro force assay with full-length and truncated cardiac myosin, we systematically perturbed ensemble activity by varying myosin availability and pharmacologically altering the fraction of force-generating motors. We find that force production depends on an optimal balance of motor engagement rather than a simple increase or decrease in active motors, demonstrating that collective mechanical output arises from coordinated interactions within the ensemble. These findings reveal emergent design principles that govern molecular motor function and establish effective motor occupancy as a key regulator of collective force generation, providing new insight into the mechanisms underlying muscle contractility.<\/jats:p>\n                <\/jats:sec>","DOI":"10.64898\/2026.07.17.739215","type":"posted-content","created":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T04:25:13Z","timestamp":1784521513000},"source":"Crossref","is-referenced-by-count":0,"title":["Motor Occupancy Defines Emergent Mechanical States in Cardiac Myosin Ensembles"],"prefix":"10.64898","author":[{"given":"Omayma Y.","family":"Alazzam","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering, University of Mississippi, University, MS, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. 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