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Here, we report the isolation and characterization of three novel lytic phages from Benin (Kp1Bj_HH11_M23, Kp2Bj_LN294_M23, and Kp10Bj_LN54_14). These Myoviruses, belonging to the genera\n                  <jats:italic>Marfavirus<\/jats:italic>\n                  and\n                  <jats:italic>Jiaodavirus<\/jats:italic>\n                  , display broad host range activity against multidrug-resistant and hypervirulent\n                  <jats:italic>K. pneumoniae<\/jats:italic>\n                  strains. Genomic analysis confirmed the absence of virulence and antimicrobial resistance genes. The phages exhibit rapid adsorption, short latency periods, and high burst sizes (119\u20132208 PFU\/cell). All three phages significantly inhibited biofilm formation and reduced established biofilms in vitro. Their stability across a wide range of temperatures and pH further supports their potential for therapeutic development. Together, these data highlight the value of locally sourced phages as candidates for tackling region-specific antimicrobial resistance challenges.\n                <\/jats:p>","DOI":"10.64898\/2026.07.25.740742","type":"posted-content","created":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T16:15:17Z","timestamp":1785428117000},"source":"Crossref","is-referenced-by-count":0,"title":["Isolation and characterization of novel\n                  <i>Klebsiella<\/i>\n                  phages from Benin and their antibiofilm activities on multidrug resistant and hypervirulent strains of\n                  <i>Klebsiella pneumoniae<\/i>"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0547-7065","authenticated-orcid":false,"given":"Alidehou Jerrold","family":"Agbankpe","sequence":"first","affiliation":[{"name":"Bacteriology Research Unit, Institut Pasteur de Guin\u00e9e, P.O Box 4416, Conakry, Guinea"},{"name":"Becky Mayer Centre for Phage Research, Division of Microbiology and Infection, University of Leicester, Leicester LE1 7RH, UK"},{"name":"Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, 01 P.O. Box 2009, Cotonou, Benin"},{"name":"Research Center for Global Health Action on Climate Change, Gender Equity, and Emerging Diseases, Pahou, Benin"},{"name":"Stellenbosch Institute for Advanced Study (STIAS), Wallenberg Research Centre at Stellenbosch University, Stellenbosch, 7600 Matieland, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-7734-2454","authenticated-orcid":false,"given":"Kafayath","family":"Fabiyi","sequence":"additional","affiliation":[{"name":"Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, 01 P.O. Box 2009, Cotonou, Benin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3991-0864","authenticated-orcid":false,"given":"Yaovi M.","family":"Gildas Houumanou","sequence":"additional","affiliation":[{"name":"Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, 01 P.O. Box 2009, Cotonou, Benin"},{"name":"Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 1870 Frederiksberg, Denmark"},{"name":"Research Center for Global Health Action on Climate Change, Gender Equity, and Emerging Diseases, Pahou, Benin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gracia Ruth","family":"Nougbologni","sequence":"additional","affiliation":[{"name":"Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, 01 P.O. Box 2009, Cotonou, Benin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roubaya","family":"Balarabe","sequence":"additional","affiliation":[{"name":"Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, 01 P.O. 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Box 2009, Cotonou, Benin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2549-7685","authenticated-orcid":false,"given":"Esther","family":"Deguenon","sequence":"additional","affiliation":[{"name":"Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, 01 P.O. Box 2009, Cotonou, Benin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9047-7299","authenticated-orcid":false,"given":"Victorien","family":"Dougnon","sequence":"additional","affiliation":[{"name":"Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, 01 P.O. Box 2009, Cotonou, Benin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Honor\u00e9","family":"Bankol\u00e9","sequence":"additional","affiliation":[{"name":"Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, 01 P.O. Box 2009, Cotonou, Benin"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8160-5954","authenticated-orcid":false,"given":"Lamine","family":"Baba-Moussa","sequence":"additional","affiliation":[{"name":"Laboratory of Biology and Molecular Typing in Microbiology, Faculty of Science and Technology, University of Abomey-Calavi, P.O. 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