{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T15:40:04Z","timestamp":1792251604455,"version":"4.4.0"},"posted":{"date-parts":[[2026,7,30]]},"group-title":"Physiology","reference-count":73,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T00:00:00Z","timestamp":1785369600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Institutes of Health","award":["R35 GM150517"],"award-info":[{"award-number":["R35 GM150517"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,10,13]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>\n                  Adipose tissue is a key metabolic organ for carbohydrate and lipid metabolism. Multiple nutrient sensing pathways and metabolic enzymes operate in adipocytes to control energy production and lipid mobilization in response to physiological conditions. Important regulators of glycolytic and lipid metabolism in many species are members of the estrogen-related receptor (ERR) family of nuclear receptors. We previously showed that ERR is required for transcriptional regulation of glycolytic and pentose phosphate pathway enzymes in adult\n                  <jats:italic>Drosophila<\/jats:italic>\n                  females, consistent with what is observed in\n                  <jats:italic>ERR<\/jats:italic>\n                  mutant males and larvae. However, the cell-type specific targets of ERR in adipose and other tissues have not been fully elucidated. Here, using a modified targeted DamID approach (NanoDam), we identified ERR occupancy specifically in adult adipocytes at the loci that encode genes involved in glycolysis, the pentose phosphate pathway, and fatty acid metabolism. Overall, our results predict that ERR centrally functions in adipose tissue to regulate multiple metabolic pathways.\n                <\/jats:p>\n                <jats:sec>\n                  <jats:title>SUMMARY<\/jats:title>\n                  <jats:p>\n                    Identification of genomic loci bound by proteins can be difficult to isolate in tissues composed of multiple cell types or if an antibody is unavailable. Here, we used a genomic approach called NanoDam to identify adult adipocyte-specific targets of the nuclear receptor, estrogen-related receptor (ERR), in\n                    <jats:italic>Drosophila<\/jats:italic>\n                    males and females. We find that ERR targets in both sexes are enriched for genes encoding enzymes involved in glycolysis and the pentose phosphate pathway, suggesting that ERR targets are similar in adult adipocytes as in larval fat. Our results will be of interest for scientists studying nuclear receptors, metabolism, and sexual dimorphism.\n                  <\/jats:p>\n                <\/jats:sec>","DOI":"10.64898\/2026.07.29.741485","type":"posted-content","created":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:50:17Z","timestamp":1785437417000},"source":"Crossref","is-referenced-by-count":0,"title":["Genomic Profiling of Estrogen-Related Receptor Identifies Adult Adipocyte-Specific Targets in\n                  <i>Drosophila<\/i>"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7694-8776","authenticated-orcid":false,"given":"Jun","family":"Yin","sequence":"first","affiliation":[{"name":"Center for Genomics and Bioinformatics, Indiana University, Bloomington, IN 47405, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1066-2687","authenticated-orcid":false,"given":"Douglas B.","family":"Rusch","sequence":"additional","affiliation":[{"name":"Center for Genomics and Bioinformatics, Indiana University, Bloomington, IN 47405, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-8139-5516","authenticated-orcid":false,"given":"Jitika S.","family":"Bhatta","sequence":"additional","affiliation":[{"name":"Department of Biology, Indiana University, Bloomington, IN 47405, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David M.","family":"Merritt","sequence":"additional","affiliation":[{"name":"Center for Genomics and Bioinformatics, Indiana University, Bloomington, IN 47405, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3120-8301","authenticated-orcid":false,"given":"Lesley N.","family":"Weaver","sequence":"additional","affiliation":[{"name":"Department of Biology, Indiana University, Bloomington, IN 47405, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026101707400697000_2026.07.29.741485v2.1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2018.02.061"},{"key":"2026101707400697000_2026.07.29.741485v2.2","doi-asserted-by":"publisher","DOI":"10.4093\/dmj.2023.0011"},{"key":"2026101707400697000_2026.07.29.741485v2.3","doi-asserted-by":"publisher","DOI":"10.1242\/dev.116467"},{"key":"2026101707400697000_2026.07.29.741485v2.4","doi-asserted-by":"publisher","DOI":"10.1038\/aps.2014.121"},{"key":"2026101707400697000_2026.07.29.741485v2.5","doi-asserted-by":"publisher","DOI":"10.1101\/gad.335281.119"},{"key":"2026101707400697000_2026.07.29.741485v2.6","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M049130"},{"key":"2026101707400697000_2026.07.29.741485v2.7","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/j.isci.2018.03.005","article-title":"Estrogen-Related Receptors Mediate the Adaptive Response of Brown Adipose Tissue to Adrenergic Stimulation","volume":"2","year":"2018","journal-title":"iScience"},{"key":"2026101707400697000_2026.07.29.741485v2.8","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-016-0924-1"},{"key":"2026101707400697000_2026.07.29.741485v2.9","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2012.221"},{"key":"2026101707400697000_2026.07.29.741485v2.10","doi-asserted-by":"publisher","DOI":"10.15252\/embr.201947928"},{"key":"2026101707400697000_2026.07.29.741485v2.11","doi-asserted-by":"crossref","first-page":"22","DOI":"10.3389\/fcvm.2020.00022","article-title":"Adipose Tissue Distribution, Inflammation and Its Metabolic Consequences, Including Diabetes and Cardiovascular Disease","volume":"7","year":"2020","journal-title":"Front Cardiovasc Med"},{"key":"2026101707400697000_2026.07.29.741485v2.12","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abg4102"},{"key":"2026101707400697000_2026.07.29.741485v2.13","first-page":"20","article-title":"Targeted DamID reveals differential binding of mammalian pluripotency factors","volume":"145","year":"2018","journal-title":"Development"},{"key":"2026101707400697000_2026.07.29.741485v2.14","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty560"},{"key":"2026101707400697000_2026.07.29.741485v2.15","doi-asserted-by":"publisher","DOI":"10.1210\/endrev\/bnaf032"},{"key":"2026101707400697000_2026.07.29.741485v2.16","doi-asserted-by":"publisher","DOI":"10.1002\/oby.20067"},{"key":"2026101707400697000_2026.07.29.741485v2.17","doi-asserted-by":"crossref","unstructured":"Dixon, E.D. , A.D. 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