{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T14:55:38Z","timestamp":1786114538949,"version":"build-2736575974"},"posted":{"date-parts":[[2026,8,3]]},"group-title":"Microbiology","reference-count":54,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T00:00:00Z","timestamp":1785715200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"award":["Project-ID 403224013\u2013SFB 1382"],"award-info":[{"award-number":["Project-ID 403224013\u2013SFB 1382"]}],"id":[{"id":"https:\/\/ror.org\/018mejw64","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,8,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Although bile acid-mediated microbiome-host interactions are known to shape gut microbial composition and function, the mechanisms by which bile acid stress influences microbial metabolic interactions remain poorly understood. Here, we investigated the interaction between two key gut microbes,\n                  <jats:italic>Bacteroides thetaiotaomicron<\/jats:italic>\n                  and\n                  <jats:italic>Collinsella aerofaciens<\/jats:italic>\n                  , under deoxycholic acid (DCA) stress. In anaerobic coculture,\n                  <jats:italic>C. aerofaciens<\/jats:italic>\n                  mitigated the inhibitory effects of DCA on\n                  <jats:italic>B. thetaiotaomicron<\/jats:italic>\n                  , primarily through DCA uptake from the medium, as confirmed by DCA quantification. Proteomic analysis showed that DCA broadly disrupted amino acid and vitamin metabolism, particularly in\n                  <jats:italic>B. thetaiotaomicron<\/jats:italic>\n                  . In contrast, coculture promoted widespread metabolic activation in\n                  <jats:italic>C. aerofaciens<\/jats:italic>\n                  , including enhanced vitamin B6 metabolism and increased production of citrulline and ornithine. These findings suggest that metabolic cooperation enhances resistance to bile acid stress and may contribute to gut microbiome resilience, with potential relevance to liver- and bile acid-related disorders.\n                <\/jats:p>","DOI":"10.64898\/2026.08.03.742426","type":"posted-content","created":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T06:20:24Z","timestamp":1785824424000},"source":"Crossref","is-referenced-by-count":0,"title":["Coculture of\n                  <i>Collinsella aerofaciens<\/i>\n                  and\n                  <i>Bacteroides thetaiotaomicron<\/i>\n                  under bile acid stress reveals vitamin B6 exchange"],"prefix":"10.64898","author":[{"given":"Yan","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Molecular Toxicology, Helmholtz-Centre for Environmental Research GmbH (UFZ), Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sven-Bastiaan","family":"Haange","sequence":"additional","affiliation":[{"name":"Department of Molecular Toxicology, Helmholtz-Centre for Environmental Research GmbH (UFZ), Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andy Mercado","family":"Gamarra","sequence":"additional","affiliation":[{"name":"Institute of Experimental Medicine, Christian-Albrechts-University Kiel, Kiel, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Len\u00ed\u010dek","sequence":"additional","affiliation":[{"name":"Institute of Medical Biochemistry and Laboratory Diagnostics, Charles University, Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah","family":"Maier","sequence":"additional","affiliation":[{"name":"Immundiagnostik AG, Bensheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robin","family":"Plail","sequence":"additional","affiliation":[{"name":"Immundiagnostik AG, Bensheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hendrik","family":"Seidl","sequence":"additional","affiliation":[{"name":"Immundiagnostik AG, Bensheim, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christoph","family":"Kaleta","sequence":"additional","affiliation":[{"name":"Institute of Experimental Medicine, Christian-Albrechts-University Kiel, Kiel, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrike","family":"Rolle-Kampczyk","sequence":"additional","affiliation":[{"name":"Department of Molecular Toxicology, Helmholtz-Centre for Environmental Research GmbH (UFZ), Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nico","family":"Jehmlich","sequence":"additional","affiliation":[{"name":"Department of Molecular Toxicology, Helmholtz-Centre for Environmental Research GmbH (UFZ), Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"von Bergen","sequence":"additional","affiliation":[{"name":"Department of Molecular Toxicology, Helmholtz-Centre for Environmental Research GmbH (UFZ), Leipzig, Germany"},{"name":"Institute of Biochemistry, Faculty of Biosciences, Pharmacy and Psychology, University of Leipzig, Leipzig, Germany"},{"name":"German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026080514053781000_2026.08.03.742426v1.1","unstructured":"Greenberger NJ , Paumgartner G , Pratt DS : Diseases of the Gallbladder and Bile Ducts. 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