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Proximity labeling identifies direct partners and neighboring proteins, thereby expanding AR-IPs to AR-proximal interacting proteins (AR-PIPs). Using proximity labeling quantitative mass spectrometry (PL-qMS), we construct a spatiotemporal atlas of the cytosolic, microsomal, and nuclear compartments in LNCaP prostate tumor cells. PL-qMS recovered 82.2% of the known AR-interactome in extranuclear compartments and 84.2% in the nucleus, identifying 4,751 AR-PIPs that remodel across an androgen time course. The retromer formed an androgen-sensitive AR-proximal interaction network (AR-PIN) verified by proximity ligation assays (PLAs). Moreover, partial VPS26A disruption attenuated androgen-regulated transcription and mislocalized the AR coactivator TMF1, defining a retromer-AR-TMF1 axis. In the nucleus, AR-PINs recover 100% of the Launonen 2021 ChIP-SICAP chromatome and reveal a PLA-verified translation-to-transcription handoff involving eIF4G and 4E-BP1. This spatiotemporal atlas provides a proximal framework for probing AR function in cells.<\/jats:p>","DOI":"10.64898\/2026.08.03.742469","type":"posted-content","created":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T16:55:13Z","timestamp":1786035313000},"source":"Crossref","is-referenced-by-count":2,"title":["A Spatiotemporal Atlas of the Androgen Receptor Proximal Interactome"],"prefix":"10.64898","author":[{"given":"Celeste C.","family":"Ptak","sequence":"first","affiliation":[{"name":"Proteomics Facility, Carver College of Medicine, University of Iowa, 431 Newton Road, Iowa City, IA 52242, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Conor","family":"O\u2019Rourke","sequence":"additional","affiliation":[{"name":"KEYENCE Corporation of America, Life Science Division, 601 Carlson Parkway, Suite 800, Minnetonka, MN 55305, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jimmy 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