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Here, we identify emperipolesis \u2013 the evolutionarily conserved process by which neutrophils pass through megakaryocytes \u2013 as a phenotypically transformative route of egress from bone marrow. By intravital microscopy and 3-D histology, we show that the rapid form emperipolesis is markedly enhanced under inflammatory conditions. Neutrophils exit from megakaryocytes directly to the blood, acquiring exosomes enriched in proteins related to metabolism, migration, and immune function. This transfer induces a distinct neutrophil phenotype characterized by enhanced glycolysis, oxidative phosphorylation, cytokine release, and longevity. Correspondingly, emperipolesis-educated neutrophils display accelerated migration\n                  <jats:italic>in vitro<\/jats:italic>\n                  and\n                  <jats:italic>in vivo<\/jats:italic>\n                  . Disrupting emperipolesis does not alter circulating neutrophil abundance but impairs neutrophil infiltration into inflamed tissues, including\n                  <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                  -infected lung. These findings establish emperipolesis as a mechanism by which megakaryocytes amplify neutrophil-mediated immunity.\n                <\/jats:p>","DOI":"10.64898\/2026.08.04.742780","type":"posted-content","created":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T04:55:11Z","timestamp":1786337711000},"source":"Crossref","is-referenced-by-count":0,"title":["Megakaryocyte emperipolesis arms neutrophils via intracellular provisioning"],"prefix":"10.64898","author":[{"given":"Julia K.","family":"Kuehn","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Division of Immunology, Boston Children\u2019s Hospital, Harvard Medical School; Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geon Ho","family":"Bae","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Division of Immunology, Boston Children\u2019s Hospital, Harvard Medical School; Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roxane","family":"Darbousset","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Division of Immunology, Boston Children\u2019s Hospital, Harvard Medical School; Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank Y.","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Division of Immunology, Boston Children\u2019s Hospital, Harvard Medical School; Boston, MA, USA"},{"name":"Division of Stem Cells and Cancer, German Cancer Research Center (DKFZ) and DKFZ-ZMBH Alliance; Heidelberg, Germany"},{"name":"Heidelberg Institute for Stem Cell Technology and Experimental Medicine (HI-STEM gGmbH); Heidelberg, Germany"},{"name":"Faculty of Biosciences, Heidelberg University; Heidelberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca M.","family":"Hall","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Division of Immunology, Boston Children\u2019s Hospital, Harvard Medical School; Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josephin E.","family":"Schiffer","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Division of Immunology, Boston Children\u2019s Hospital, Harvard Medical School; 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