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Yet capturing the karyotype or three-dimensional architecture of chromatin in living cells remains limited by the difficulty in labeling endogenous loci non-invasively. The most widely used tools rely on dCas9, a bulky protein whose persistent DNA binding interferes with DNA and RNA metabolism, causes DNA damage, and is hard to multiplex. We developed\n                  <jats:italic>ZATELLITE<\/jats:italic>\n                  , an AI-enabled tool to target endogenous repetitive sequences with fluorescently-tagged synthetic zinc fingers.\n                  <jats:italic>ZATELLITE<\/jats:italic>\n                  probes have key advantages over dCas9: they are smaller, easier to multiplex and, critically, they do not cause DNA damage or chromosomal abnormalities, even after long-term labeling. We generated a collection of\n                  <jats:italic>ZATELLITE<\/jats:italic>\n                  probes to label the centromeres of nearly all human chromosomes, enabling the capture of genome organization and karyotype alterations in real time in living cells. Finally, we show that\n                  <jats:italic>ZATELLITE<\/jats:italic>\n                  can be used for simultaneous labeling and epigenetic editing of centromeres. Thus,\n                  <jats:italic>ZATELLITE<\/jats:italic>\n                  is a non-toxic, versatile tool for genome visualization and manipulation.\n                <\/jats:p>","DOI":"10.64898\/2026.08.04.742839","type":"posted-content","created":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T13:40:40Z","timestamp":1785937240000},"source":"Crossref","is-referenced-by-count":0,"title":["<i>ZATELLITE:<\/i>\n                  a toolkit to visualize and manipulate human centromeres in live cells using synthetic zinc fingers"],"prefix":"10.64898","author":[{"given":"Nestor","family":"Saiz","sequence":"first","affiliation":[{"name":"Institute for Systems Genetics, NYU Grossman School of Medicine, New York, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aleah","family":"Goldberg","sequence":"additional","affiliation":[{"name":"Institute for Systems Genetics, NYU Grossman School of Medicine, New York, NY, 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