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Here we introduce a biochemical probe,\n                  <jats:italic>O<\/jats:italic>\n                  -(trimethylammoniobutyl)-hydroxylamine (TAMOHA), that enables comprehensive profiling of electrophilic species such as coenzyme-A esters, ketones, and aldehydes. TAMOHA incorporates a highly nucleophilic hydroxyl amine that reacts quickly with electrophilic species upon tissue lysis, trapping them as stable derivatives that feature a tetraalkylammonium moiety whose constitutive charge and characteristic MS\n                  <jats:sup>2<\/jats:sup>\n                  fragmentation fingerprint enable their highly sensitive detection. Using TAMOHA to survey the electrophilomes of\n                  <jats:italic>E. coli, C. elegans<\/jats:italic>\n                  , and mouse revealed several thousand electrophilic metabolites, most of which have not been characterized. We then demonstrate trapping of electrophilic metabolites with TAMOHA in the context of specific biochemical pathways, confirming previously proposed functions of two fatty acid metabolism enzymes, detecting formaldehyde production in mice, and providing new insights into the biosynthesis of ascaroside pheromones in\n                  <jats:italic>C. elegans<\/jats:italic>\n                  . We anticipate that use of TAMOHA for the profiling of electrophilic species will help clarify enzyme function and uncover previously elusive biochemical mechanisms in a wide range of biological systems.\n                <\/jats:p>\n                <jats:sec>\n                  <jats:title>Table of Contents artwork<\/jats:title>\n                  <jats:fig id=\"ufig1\" position=\"float\" fig-type=\"figure\" orientation=\"portrait\">\n                    <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"743530v1_ufig1\" position=\"float\" orientation=\"portrait\"\/>\n                  <\/jats:fig>\n                <\/jats:sec>","DOI":"10.64898\/2026.08.07.743530","type":"posted-content","created":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T13:40:20Z","timestamp":1786369220000},"source":"Crossref","is-referenced-by-count":1,"title":["Omics-scale capture of electrophilic metabolites"],"prefix":"10.64898","author":[{"given":"Allen F.","family":"Schroeder","sequence":"first","affiliation":[{"name":"Boyce Thompson Institute and Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Yu","sequence":"additional","affiliation":[{"name":"Boyce Thompson Institute and Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Turk","sequence":"additional","affiliation":[{"name":"Boyce Thompson Institute and Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henry H.","family":"Le","sequence":"additional","affiliation":[{"name":"Boyce Thompson Institute and Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"LeClair","sequence":"additional","affiliation":[{"name":"Boyce Thompson Institute and Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marissa A.","family":"Fontaine","sequence":"additional","affiliation":[{"name":"Boyce Thompson Institute and Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas W.","family":"Cheng","sequence":"additional","affiliation":[{"name":"Division of Nutritional Sciences, Cornell University, Ithaca, New York 14853, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saeideh","family":"Azad","sequence":"additional","affiliation":[{"name":"Division of Nutritional Sciences, Cornell University, Ithaca, New York 14853, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher N.","family":"Parkhurst","sequence":"additional","affiliation":[{"name":"Jill Roberts Institute for Research in Inflammatory Bowel Disease, Division of Gastroenterology and Hepatology, Joan and Sanford I. Weill Department of Medicine, Weill Cornell Medicine, New York, NY, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason","family":"Pan","sequence":"additional","affiliation":[{"name":"Boyce Thompson Institute and Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Artis","sequence":"additional","affiliation":[{"name":"Jill Roberts Institute for Research in Inflammatory Bowel Disease, Division of Gastroenterology and Hepatology, Joan and Sanford I. 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