{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T01:27:35Z","timestamp":1786584455239,"version":"build-2736575974"},"posted":{"date-parts":[[2026,8,10]]},"group-title":"Cell Biology","reference-count":35,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T00:00:00Z","timestamp":1786320000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"funder":[{"award":["ANR-21-CE14-0026-01"],"award-info":[{"award-number":["ANR-21-CE14-0026-01"]}],"id":[{"id":"https:\/\/ror.org\/00rbzpz17","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,8,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Muscle ageing is characterized by evolutionarily conserved subcellular alterations across diverse organisms. In\n                  <jats:italic>Caenorhabditis elegans<\/jats:italic>\n                  , the decline in sarcomeric gene expression is among the earliest detectable ageing-associated changes, emerging at the onset of adulthood. To identify causal regulators of muscle ageing in an unbiased manner, we developed a genetic screening strategy that enables visual monitoring of muscle ageing at both cellular and organismal scales. Using this approach, we identified a mutation that delays the age-associated loss of sarcomeric transcripts. Unexpectedly, the mutation maps to the troponin I gene\n                  <jats:italic>unc-27<\/jats:italic>\n                  , which encodes a conserved regulator of muscle contraction not previously implicated in gene regulation. The mutation alters a single amino acid within a predicted nuclear localization signal (NLS). We found that multiple NLS motifs mediate the active transport of UNC-27 into muscle nuclei from early adulthood onward. Disruption of UNC-27 nuclear localization preserves sarcomeric gene expression during ageing and delays early hallmarks of muscle decline, including proteostatic imbalance and mitochondrial fragmentation. Transcriptomic analyses further revealed that nuclear UNC-27 selectively regulates the expression of genes encoding structural components of the muscle apparatus in adult animals.\n                <\/jats:p>\n                <jats:p>These results support the existence of a homeostatic sarcomere surveillance pathway, in which a structural protein unexpectedly acquires a transcriptional regulatory role in response to age-associated physiological state. The conservation of NLS motifs in mammalian UNC-27 orthologues suggests that this mechanism may be evolutionarily conserved, with potential relevance to human muscle physiology and disease.<\/jats:p>","DOI":"10.64898\/2026.08.08.743652","type":"posted-content","created":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T14:55:19Z","timestamp":1786373719000},"source":"Crossref","is-referenced-by-count":0,"title":["A nuclear role for the contractile protein troponin I\/UNC-27 in regulating muscle aging in\n                  <i>C. elegans<\/i>"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-9167-3280","authenticated-orcid":false,"given":"Allan","family":"Alcolei","sequence":"first","affiliation":[{"name":"Universit\u00e9 Lyon 1, MeLiS, CNRS UMR 5284, INSERM U1314, Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manon","family":"Froment","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Lyon 1, MeLiS, CNRS UMR 5284, INSERM U1314, Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Molin","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Lyon 1, MeLiS, CNRS UMR 5284, INSERM U1314, Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8955-2423","authenticated-orcid":false,"given":"Charline","family":"Roy","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Lyon 1, MeLiS, CNRS UMR 5284, INSERM U1314, Lyon, France"},{"name":"Animal Physiology and Neurobiology, Department of Biology, University of Leuven (KU Leuven), Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9128-8809","authenticated-orcid":false,"given":"Romain","family":"Bulteau","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Lyon, ENS de Lyon LBMC, CNRS UMR 5239, INSERM 1293, Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3088-7621","authenticated-orcid":false,"given":"Jean-Louis","family":"Bessereau","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Lyon 1, MeLiS, CNRS UMR 5284, INSERM U1314, Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4173-8314","authenticated-orcid":false,"given":"Florence","family":"Solari","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Lyon 1, MeLiS, CNRS UMR 5284, INSERM U1314, Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026081217504036000_2026.08.08.743652v1.1","doi-asserted-by":"publisher","DOI":"10.1101\/2024.01.22.576648"},{"key":"2026081217504036000_2026.08.08.743652v1.2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diff.2011.10.002"},{"key":"2026081217504036000_2026.08.08.743652v1.3","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-22058-7"},{"key":"2026081217504036000_2026.08.08.743652v1.4","doi-asserted-by":"publisher","DOI":"10.1002\/cm.21281"},{"key":"2026081217504036000_2026.08.08.743652v1.5","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a005728"},{"key":"2026081217504036000_2026.08.08.743652v1.6","doi-asserted-by":"publisher","DOI":"10.1126\/science.1168275"},{"key":"2026081217504036000_2026.08.08.743652v1.7","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2010.08.017"},{"key":"2026081217504036000_2026.08.08.743652v1.8","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-022-01540-0"},{"key":"2026081217504036000_2026.08.08.743652v1.9","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.18459"},{"key":"2026081217504036000_2026.08.08.743652v1.10","doi-asserted-by":"publisher","DOI":"10.3390\/cells8070741"},{"key":"2026081217504036000_2026.08.08.743652v1.11","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.75529"},{"key":"2026081217504036000_2026.08.08.743652v1.12","doi-asserted-by":"crossref","unstructured":"Fabrizio, P. , Alcolei, A. , & Solari, F. 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