{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T23:24:32Z","timestamp":1787009072133,"version":"build-2736575974"},"posted":{"date-parts":[[2026,8,14]]},"group-title":"Neuroscience","reference-count":63,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T00:00:00Z","timestamp":1786665600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"award":["R01NS123285"],"award-info":[{"award-number":["R01NS123285"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"award":["U01DA053691"],"award-info":[{"award-number":["U01DA053691"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,8,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Hundreds of neuronal cell types and subtypes have been identified through transcriptome profiling of the mammalian brain and are thought to arise from lineage-restricted neural progenitors during early development. However, how neurons might further diversify their transcriptional identities during postmitotic development and through adulthood remains poorly understood. Here, we combine genetic and biochemical approaches to uncover granule neuron subpopulations in the anterior cerebellum, which we term C1 and C2. We find that a large proportion of granule neurons predominantly express the C2 gene program during early postmitotic differentiation, but that C2 genes are downregulated in a subset of neurons during late postnatal development, leading to comparable proportions of C1 and C2 neurons in adulthood. Using an\n                  <jats:italic>in vivo<\/jats:italic>\n                  genetic mini-screen, we identify calcium signaling pathways, together with the transcription factor ETV1, that establish the transcriptional program of C2 neurons. Finally, C1 and C2 granule neurons are differentially engaged during behavior and this reflects their roles in cerebellar-dependent associative learning. Together, these findings reveal postmitotic mechanisms that continue to shape neuronal identity in the brain.\n                <\/jats:p>","DOI":"10.64898\/2026.08.09.743777","type":"posted-content","created":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T23:30:12Z","timestamp":1786750212000},"source":"Crossref","is-referenced-by-count":0,"title":["Postmitotic Regulation of Neuronal Subpopulations in the Cerebellum"],"prefix":"10.64898","author":[{"given":"Pamela","family":"Valnegri","sequence":"first","affiliation":[{"name":"Department of Neurobiology, Northwestern University, Evanston, IL, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shin-ichiro","family":"Fujita","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, Northwestern University, Evanston, IL, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-2645-852X","authenticated-orcid":false,"given":"Tomoko","family":"Yamada","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, Northwestern University, Evanston, IL, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6201-8818","authenticated-orcid":false,"given":"Yue","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Neurobiology, Northwestern University, Evanston, IL, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026081715300708000_2026.08.09.743777v1.1","doi-asserted-by":"crossref","unstructured":"West, E.R. , et al. Spatiotemporal patterns of neuronal subtype genesis suggest hierarchical development of retinal diversity. Cell Rep 38, 110191 (2022).","DOI":"10.1016\/j.celrep.2021.110191"},{"key":"2026081715300708000_2026.08.09.743777v1.2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-04237-0"},{"key":"2026081715300708000_2026.08.09.743777v1.3","doi-asserted-by":"crossref","unstructured":"Telley, L. , et al. Temporal patterning of apical progenitors and their daughter neurons in the developing neocortex. Science 364 (2019).","DOI":"10.1126\/science.aav2522"},{"key":"2026081715300708000_2026.08.09.743777v1.4","doi-asserted-by":"crossref","unstructured":"Soldatov, R. , et al. Spatiotemporal structure of cell fate decisions in murine neural crest. Science 364 (2019).","DOI":"10.1126\/science.aas9536"},{"key":"2026081715300708000_2026.08.09.743777v1.5","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-022-04564-w"},{"key":"2026081715300708000_2026.08.09.743777v1.6","doi-asserted-by":"crossref","unstructured":"Bandler, R.C. & Mayer, C . Deciphering inhibitory neuron development: The paths to diversity. 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Homologous organization of cerebellar pathways to sensory, motor, and associative forebrain. Cell Rep 36, 109721 (2021).","DOI":"10.1016\/j.celrep.2021.109721"},{"key":"2026081715300708000_2026.08.09.743777v1.15","doi-asserted-by":"crossref","unstructured":"Fujita, H. , Kodama, T. & du Lac, S . Modular output circuits of the fastigial nucleus for diverse motor and nonmotor functions of the cerebellar vermis. 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