{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T04:28:03Z","timestamp":1786595283867,"version":"build-2736575974"},"posted":{"date-parts":[[2026,8,10]]},"group-title":"Synthetic Biology","reference-count":43,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T00:00:00Z","timestamp":1786320000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000928","name":"Welch Foundation","doi-asserted-by":"crossref","award":["C-2167-20230405"],"award-info":[{"award-number":["C-2167-20230405"]}],"id":[{"id":"10.13039\/100000928","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000928","name":"Welch Foundation","doi-asserted-by":"crossref","award":["F-1155"],"award-info":[{"award-number":["F-1155"]}],"id":[{"id":"10.13039\/100000928","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R35GM139658"],"award-info":[{"award-number":["R35GM139658"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,8,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Selenocysteine (Sec), the 21\n                  <jats:sup>st<\/jats:sup>\n                  amino acid, is a rare non-canonical amino acid that represents an attractive target for protein engineering due to its desirable chemical properties such as high affinity for metals, strong nucleophilicity, and reversible covalent bond formation. To bypass the natural constraints on Sec placement within proteins, several strategies have been developed to rewire the native translational machinery to enable site-specific incorporation. However, these usually abolish the quality control mechanism that excludes the serine-charged selenocysteinyl-tRNA (Ser-tRNA\n                  <jats:sup>Sec<\/jats:sup>\n                  ), the immediate biosynthetic precursor, from translation resulting in heterogenous protein species. This challenge is confounded by a lack of genetic tools to accurately report the selenylation state of the tRNA pool as most are blind to competing process of Ser incorporation, which can only be observed using analytical methods. To resolve this issue, we have developed a new fluorescent reporter,\n                  <jats:underline>Se<\/jats:underline>\n                  lenocysteine\n                  <jats:underline>A<\/jats:underline>\n                  djusted\n                  <jats:underline>R<\/jats:underline>\n                  atiometric\n                  <jats:underline>Ch<\/jats:underline>\n                  romophore (SeARCh), which exhibits two distinct spectral outputs dependent on the incorporation of either Ser (red) or Sec (green). Using SeARCh, we define several factors which influence the observed Sec:Ser ratio and construct a new hybrid biosynthetic pathway with improved performance, achieving 90% Sec incorporation. Furthermore, SeARCh displays unusually complex mass spectra due to the isotope distribution of selenium and heterogenous nature of the protein in solution and we report specific methods to account for this behaviour and precisely quantify the rare Ser-containing species found at high Sec incorporation efficiencies. Our findings suggest that the equilibrium between selenoprotein and tRNA\n                  <jats:sup>Sec<\/jats:sup>\n                  expression levels is a key driver of incorporation efficiency and implies a process that is broadly biosynthetically constrained. Collectively these tools represent a significant advance in the metrology of selenocysteine biosynthesis and incorporation and can be used to inform and standardize future engineering efforts.\n                <\/jats:p>","DOI":"10.64898\/2026.08.09.743795","type":"posted-content","created":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T08:45:11Z","timestamp":1786437911000},"source":"Crossref","is-referenced-by-count":0,"title":["A Quantitative Two-Channel Genetic Reporter for Selenocysteine Biosynthesis and Incorporation"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8422-2863","authenticated-orcid":false,"given":"Andrew","family":"Gilmour","sequence":"first","affiliation":[{"name":"Systems, Synthetic, and Physical Biology, Rice University, Houston, TX"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1147-6405","authenticated-orcid":false,"given":"Qiyao","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, Rice University, Houston, TX"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3664-7856","authenticated-orcid":false,"given":"Jessica","family":"Hellinger","sequence":"additional","affiliation":[{"name":"Department of Chemistry, The University of Texas at Austin, Austin, TX"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7212-9802","authenticated-orcid":false,"given":"Devon L.","family":"Kulhanek","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering, Rice University, Houston, TX"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9866-6465","authenticated-orcid":false,"given":"Zach","family":"Jansen","sequence":"additional","affiliation":[{"name":"Systems, Synthetic, and Physical Biology, Rice University, Houston, TX"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2364-8136","authenticated-orcid":false,"given":"Katelyn M.","family":"Baumer","sequence":"additional","affiliation":[{"name":"Shared Equipment Authority, Rice University, Houston, TX"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3207-0217","authenticated-orcid":false,"given":"Jennifer S.","family":"Brodbelt","sequence":"additional","affiliation":[{"name":"Department of Chemistry, The University of Texas at Austin, Austin, TX"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0356-5790","authenticated-orcid":false,"given":"Ross","family":"Thyer","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering, Rice University, Houston, TX"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026081220501431000_2026.08.09.743795v1.1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1991.tb00722.x"},{"key":"2026081220501431000_2026.08.09.743795v1.2","doi-asserted-by":"publisher","DOI":"10.1021\/bi400462j"},{"key":"2026081220501431000_2026.08.09.743795v1.3","doi-asserted-by":"crossref","first-page":"624","DOI":"10.1038\/nbt.4154","article-title":"Custom selenoprotein production enabled by laboratory evolution of recoded bacterial strains","volume":"36","year":"2018","journal-title":"7. 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