{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T07:26:36Z","timestamp":1786778796432,"version":"build-2736575974"},"posted":{"date-parts":[[2026,8,13]]},"group-title":"Physiology","reference-count":50,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"funder":[{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Disease","doi-asserted-by":"crossref","award":["F99DK143563"],"award-info":[{"award-number":["F99DK143563"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Disease","doi-asserted-by":"crossref","award":["R01DK125543"],"award-info":[{"award-number":["R01DK125543"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"crossref","award":["P20-GM-135007"],"award-info":[{"award-number":["P20-GM-135007"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000050","name":"National Heart Lung and Blood Institute","doi-asserted-by":"crossref","award":["1K01HL167052"],"award-info":[{"award-number":["1K01HL167052"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000968","name":"American Heart Association","doi-asserted-by":"crossref","award":["23CDA1050558"],"award-info":[{"award-number":["23CDA1050558"]}],"id":[{"id":"10.13039\/100000968","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,8,13]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>\n                  The urinary bladder functions to store and release urine, yet how the sensation of bladder fullness is conveyed and perceived to the central nervous system is not understood. During bladder filling, the detrusor smooth muscle (DSM) generates phasic contractions, resulting in pressure fluctuations within the bladder. These transient pressure events drive bursts of afferent nerve activity, yet the underlying mechanism leading to rhythmic contractions remains unclear. Here, we examined the role of Gq protein-coupled receptor (GqPCR) activity on DSM excitability and contractility. Using ex vivo pressurized urinary bladder preparations and sharp microelectrode experiments on bladder strips from mice, we evaluated whole bladder transient pressure events, whole bladder DSM Ca\n                  <jats:sup>2+<\/jats:sup>\n                  activity, and membrane potential in bladder strips. We found that global inhibition of urinary bladder GqPCR activity with YM-254890 abates phasic contractility and transient pressure events through a reduction in DSM Ca\n                  <jats:sup>2+<\/jats:sup>\n                  activity and propagation of Ca\n                  <jats:sup>2+<\/jats:sup>\n                  waves. Further, we found inhibition of GqPCR significantly hyperpolarizes DSM, reducing action potentials and decreasing excitability, and activation of protein kinase C restores membrane potential to baseline levels. These findings highlight that GqPCR activity mediates DSM excitability and contractility in such a way as to result in phasic detrusor contractions and transient pressure events.\n                <\/jats:p>","DOI":"10.64898\/2026.08.10.743960","type":"posted-content","created":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T13:30:12Z","timestamp":1786627812000},"source":"Crossref","is-referenced-by-count":0,"title":["G-protein coupled receptor activity mediates detrusor smooth muscle phasic contractility through regulation of membrane potential"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0232-8339","authenticated-orcid":false,"given":"Jason L.","family":"Rengo","sequence":"first","affiliation":[{"name":"Dept. of Pharmacology, Larner College of Medicine, University of Vermont, Burlington, VT 05405"},{"name":"Cellular, Molecular, and Biomedical Sciences Graduate Program, University of Vermont, Burlington, VT 05405"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas J.","family":"Heppner","sequence":"additional","affiliation":[{"name":"Dept. of Pharmacology, Larner College of Medicine, University of Vermont, Burlington, VT 05405"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grant W.","family":"Hennig","sequence":"additional","affiliation":[{"name":"Dept. of Pharmacology, Larner College of Medicine, University of Vermont, Burlington, VT 05405"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7540-0306","authenticated-orcid":false,"given":"Nicholas R.","family":"Klug","sequence":"additional","affiliation":[{"name":"Dept. of Pharmacology, Larner College of Medicine, University of Vermont, Burlington, VT 05405"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sloane","family":"Stamp","sequence":"additional","affiliation":[{"name":"Dept. of Pharmacology, Larner College of Medicine, University of Vermont, Burlington, VT 05405"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark T.","family":"Nelson","sequence":"additional","affiliation":[{"name":"Dept. of Pharmacology, Larner College of Medicine, University of Vermont, Burlington, VT 05405"},{"name":"Division of Cardiovascular Sciences, University of Manchester, Manchester, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerald M.","family":"Herrera","sequence":"additional","affiliation":[{"name":"Dept. of Pharmacology, Larner College of Medicine, University of Vermont, Burlington, VT 05405"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026081423501052000_2026.08.10.743960v1.1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00038.2003"},{"key":"2026081423501052000_2026.08.10.743960v1.2","first-page":"745638","article-title":"Signaling Pathways Mediating Bradykinin-Induced Contraction in Murine and Human Detrusor 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