{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T21:24:07Z","timestamp":1787088247108,"version":"build-2736575974"},"posted":{"date-parts":[[2026,8,15]]},"group-title":"Biophysics","reference-count":15,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T00:00:00Z","timestamp":1786752000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"funder":[{"award":["GM134113"],"award-info":[{"award-number":["GM134113"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,8,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>Eukaryotic translation initiation is tightly regulated by interactions among translation initiation factors (eIFs) that ensure accurate start codon selection. The translation regulator, eIF5 mimic protein 1 (5MP1) contributes to this process by competing with eIF5 for binding to eIF2, thereby increasing the stringency of translation initiation. Despite its important regulatory role and emerging involvement in tumorigenesis, structural information on human 5MP1 remains limited. Here, we report the near-complete backbone and partial side-chain NMR resonance assignments of the C-terminal domain of human 5MP1 (residues 250\u2013419), carrying a W404E substitution that disrupts dimerization. The WT protein forms a dimer at NMR concentrations, which increases the effective size of the protein and also causes disappearance of peaks corresponding to aminoacids at the dimer interface due to conformational exchange.<\/jats:p>\n                <jats:p>\n                  Backbone resonance assignments were completed for 96.4% of the non-proline residues. Secondary structure was analyzed using Chemical Shift Index (CSI) and compared with the AlphaFold structural model. Regions of disagreement between the experimental and computational secondary structure assignments were further examined using\n                  <jats:sup>15<\/jats:sup>\n                  N-NOESY-HSQC spectra, allowing experimental validation of local structural features. While the AlphaFold model accurately reproduces the overall fold of the 5MP1 C-terminal domain, several localized discrepancies were identified, particularly near the N- and C-terminal regions of the domain, where experimental NMR data support alternative secondary structure assignments. These resonance assignments and experimentally validated structural features provide a foundation for future investigations of the molecular interactions, dynamics, and functions of 5MP1 in translation initiation.\n                <\/jats:p>","DOI":"10.64898\/2026.08.11.744028","type":"posted-content","created":{"date-parts":[[2026,8,16]],"date-time":"2026-08-16T04:45:19Z","timestamp":1786855519000},"source":"Crossref","is-referenced-by-count":0,"title":["NMR assignments and secondary structure analysis of the human 5MP1 C-terminal domain"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-6918-5466","authenticated-orcid":false,"given":"Ay\u015fenur","family":"\u015eeker","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Physiology and Biophysics, Boston University Chobanian & Avedisian School of Medicine, 700 Albany St., W336, Boston, MA 02118, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-0180-607X","authenticated-orcid":false,"given":"Simran","family":"Anand","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Physiology and Biophysics, Boston University Chobanian & Avedisian School of Medicine, 700 Albany St., W336, Boston, MA 02118, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9901-3500","authenticated-orcid":false,"given":"Assen","family":"Marintchev","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Physiology and Biophysics, Boston University Chobanian & Avedisian School of Medicine, 700 Albany St., W336, Boston, MA 02118, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026081813250542000_2026.08.11.744028v1.1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00197809"},{"key":"2026081813250542000_2026.08.11.744028v1.2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv494"},{"key":"2026081813250542000_2026.08.11.744028v1.3","doi-asserted-by":"publisher","DOI":"10.1038\/S41586-021-03819-2"},{"key":"2026081813250542000_2026.08.11.744028v1.4","unstructured":"Keller RLJ (2004) Optimizing the process of nuclear magnetic resonance spectrum analysis and computer aided resonance assignment. In ETH Zurich"},{"key":"2026081813250542000_2026.08.11.744028v1.5","doi-asserted-by":"publisher","DOI":"10.1002\/pro.5560040819"},{"key":"2026081813250542000_2026.08.11.744028v1.6","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw559"},{"key":"2026081813250542000_2026.08.11.744028v1.7","doi-asserted-by":"crossref","first-page":"5125","DOI":"10.7150\/jca.53282","article-title":"BZW2\/5MP1 acts as a promising target in hepatocellular carcinoma","volume":"12","year":"2021","journal-title":"J Cancer"},{"key":"2026081813250542000_2026.08.11.744028v1.8","doi-asserted-by":"crossref","first-page":"e70016","DOI":"10.1002\/jgm.70016","article-title":"USF2-Mediated Transcription of BZW2 Contributes to CRC Malignant Progression by Affecting LAMP3","volume":"27","year":"2025","journal-title":"J Gene Med"},{"key":"2026081813250542000_2026.08.11.744028v1.9","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0192648"},{"key":"2026081813250542000_2026.08.11.744028v1.10","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1042\/BST20253022","article-title":"Exploring the interaction dynamics of eukaryotic translation initiation factor 2","volume":"53","year":"2025","journal-title":"Biochem Soc Trans"},{"key":"2026081813250542000_2026.08.11.744028v1.11","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(07)30012-8"},{"key":"2026081813250542000_2026.08.11.744028v1.12","doi-asserted-by":"publisher","DOI":"10.1021\/bi051271v"},{"key":"2026081813250542000_2026.08.11.744028v1.13","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr339"},{"key":"2026081813250542000_2026.08.11.744028v1.14","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx808"},{"key":"2026081813250542000_2026.08.11.744028v1.15","doi-asserted-by":"publisher","DOI":"10.1093\/NAR\/GKAB1061"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.64898\/2026.08.11.744028","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T20:25:14Z","timestamp":1787084714000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.64898\/2026.08.11.744028"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,15]]},"references-count":15,"URL":"https:\/\/doi.org\/10.64898\/2026.08.11.744028","relation":{},"subject":[],"published":{"date-parts":[[2026,8,15]]},"subtype":"preprint"}}