{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T18:39:52Z","timestamp":1786732792653,"version":"build-2736575974"},"posted":{"date-parts":[[2026,8,12]]},"group-title":"Genetics","reference-count":96,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T00:00:00Z","timestamp":1786492800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,8,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Restricting the localization of centromere-specific histone H3 variant Cse4 (CENP-A in humans) to centromeric chromatin is essential for chromosome segregation. Mislocalization of overexpressed Cse4\/CENP-A to non-centromeric regions contributes to chromosomal instability (CIN) in model organisms and human cells. CIN is an important hallmark of many cancers and hence defining mechanisms that prevent mislocalization of Cse4 is clinically significant. Here we report a role for\n                  <jats:italic>YRA2<\/jats:italic>\n                  (\n                  <jats:underline>Y<\/jats:underline>\n                  east\n                  <jats:underline>R<\/jats:underline>\n                  NA\n                  <jats:underline>A<\/jats:underline>\n                  nnealing Protein 2) in ubiquitin mediated proteolysis of Cse4 to prevent its mislocalization for chromosomal stability.\n                  <jats:italic>YRA2<\/jats:italic>\n                  was identified in a genome-wide screen for gene deletions that exhibit synthetic dosage lethality (SDL) upon overexpression of\n                  <jats:italic>CSE4<\/jats:italic>\n                  (\n                  <jats:italic>GALCSE4<\/jats:italic>\n                  ). We determined that\n                  <jats:italic>yra2\u0394<\/jats:italic>\n                  strains exhibit increased Cse4 stability, enriched Cse4 chromatin association, reduced Cse4 ubiquitination, Cse4 mislocalization, and CIN. Defects in interaction of E3 ubiquitin ligase Psh1 with Cse4 contributes to stability of Cse4 in\n                  <jats:italic>yra2\u0394<\/jats:italic>\n                  strains. Consistent with these results, overexpression of\n                  <jats:italic>PSH1<\/jats:italic>\n                  suppresses\n                  <jats:italic>GALCSE4<\/jats:italic>\n                  SDL in\n                  <jats:italic>yra2\u0394<\/jats:italic>\n                  strain. We determined that Yra2 mediated proteolysis of Cse4 is independent of its RNA related functions as strain deleted for the C-terminal ChTOP domain of Yra2 with an intact N-terminal RNA binding domain exhibits\n                  <jats:italic>GALCSE4<\/jats:italic>\n                  SDL and defects in Cse4 proteolysis. Furthermore, poly(A)\n                  <jats:sup>+<\/jats:sup>\n                  RNA export mutants in\n                  <jats:italic>YRA1<\/jats:italic>\n                  (\n                  <jats:italic>yra1-2<\/jats:italic>\n                  ) and\n                  <jats:italic>MEX67<\/jats:italic>\n                  (\n                  <jats:italic>mex67-5),<\/jats:italic>\n                  that interact with Yra2, do not exhibit\n                  <jats:italic>GALCSE4 SDL<\/jats:italic>\n                  and defects in RNA export are not observed in\n                  <jats:italic>yra2\u0394<\/jats:italic>\n                  cells. In summary, we have defined a key role for Yra2 in preventing mislocalization of Cse4 by facilitating its proteolysis to preserve chromosomal stability.\n                <\/jats:p>\n                <jats:sec>\n                  <jats:title>Article summary<\/jats:title>\n                  <jats:p>Accurate segregation of chromosomes during cell division is essential because segregation errors are linked to cancer and developmental disorders. We investigated how cells prevent mislocalization of centromere-specific histone H3 variant Cse4, which is essential for faithful chromosome segregation. We found that the yeast RNA annealing protein Yra2 prevents Cse4 mislocalization by promoting Psh1 mediated ubiquitination and degradation of Cse4. Cells lacking Yra2 showed increased stability of Cse4, enhanced chromatin enrichment with mislocalization to non-centromeric regions and CIN. These defects were suppressed by induction of Psh1. Our findings reveal a novel role for Yra2 in regulating Cse4 levels for chromosomal stability.<\/jats:p>\n                <\/jats:sec>","DOI":"10.64898\/2026.08.11.744167","type":"posted-content","created":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T03:40:12Z","timestamp":1786592412000},"source":"Crossref","is-referenced-by-count":0,"title":["Yra2 regulates proteolysis of Cse4 to prevent its mislocalization to non-centromeric regions for chromosomal stability in budding yeast"],"prefix":"10.64898","author":[{"given":"Prashant K.","family":"Mishra","sequence":"first","affiliation":[{"name":"Yeast Genome Stability Section, Genetics Branch, NCI\/NIH, Bethesda, MD, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kentaro","family":"Ohkuni","sequence":"additional","affiliation":[{"name":"Yeast Genome Stability Section, Genetics Branch, NCI\/NIH, Bethesda, MD, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascal","family":"Raymond","sequence":"additional","affiliation":[{"name":"University of Montreal, Montreal, QC, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7906-2604","authenticated-orcid":false,"given":"Michael","family":"Costanzo","sequence":"additional","affiliation":[{"name":"University of Toronto, Toronto, ON, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles","family":"Boone","sequence":"additional","affiliation":[{"name":"University of Toronto, Toronto, ON, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0067-0093","authenticated-orcid":false,"given":"Daniel R.","family":"Zenklusen","sequence":"additional","affiliation":[{"name":"University of Montreal, Montreal, QC, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Munira A.","family":"Basrai","sequence":"additional","affiliation":[{"name":"Yeast Genome Stability Section, Genetics Branch, NCI\/NIH, Bethesda, MD, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026081411153075000_2026.08.11.744167v1.1","doi-asserted-by":"publisher","DOI":"10.1038\/nature09594"},{"key":"2026081411153075000_2026.08.11.744167v1.2","doi-asserted-by":"publisher","DOI":"10.1186\/1476-4598-8-119"},{"key":"2026081411153075000_2026.08.11.744167v1.3","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.118.301745"},{"key":"2026081411153075000_2026.08.11.744167v1.4","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2023.113415"},{"key":"2026081411153075000_2026.08.11.744167v1.5","doi-asserted-by":"publisher","DOI":"10.1186\/1756-8935-8-2"},{"key":"2026081411153075000_2026.08.11.744167v1.6","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.108.088518"},{"key":"2026081411153075000_2026.08.11.744167v1.7","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.113.149898"},{"key":"2026081411153075000_2026.08.11.744167v1.8","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1008597"},{"key":"2026081411153075000_2026.08.11.744167v1.9","doi-asserted-by":"publisher","DOI":"10.1038\/s44318-024-00093-6"},{"key":"2026081411153075000_2026.08.11.744167v1.10","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1080\/10985549.2025.2487010","article-title":"Protein Phosphatase 1 Regulatory Subunit PNUTS Prevents CENP-A Mislocalization and Chromosomal Instability","volume":"45","year":"2025","journal-title":"Mol Cell Biol"},{"key":"2026081411153075000_2026.08.11.744167v1.11","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-06545-y"},{"key":"2026081411153075000_2026.08.11.744167v1.12","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.112.145276"},{"key":"2026081411153075000_2026.08.11.744167v1.13","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-58592-5_21"},{"key":"2026081411153075000_2026.08.11.744167v1.14","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E12-12-0893"},{"key":"2026081411153075000_2026.08.11.744167v1.15","doi-asserted-by":"publisher","DOI":"10.1016\/j.tig.2012.02.003"},{"key":"2026081411153075000_2026.08.11.744167v1.16","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2007.05.013"},{"key":"2026081411153075000_2026.08.11.744167v1.17","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.2598"},{"key":"2026081411153075000_2026.08.11.744167v1.18","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2012.342"},{"key":"2026081411153075000_2026.08.11.744167v1.19","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-08923-w"},{"key":"2026081411153075000_2026.08.11.744167v1.20","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M115.701813"},{"key":"2026081411153075000_2026.08.11.744167v1.21","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.118.301305"},{"key":"2026081411153075000_2026.08.11.744167v1.22","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2004.10.024"},{"key":"2026081411153075000_2026.08.11.744167v1.23","unstructured":"Costanzo, M. , B. 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