{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T05:24:02Z","timestamp":1787203442104,"version":"build-2736575974"},"posted":{"date-parts":[[2026,8,17]]},"group-title":"Cell Biology","reference-count":103,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T00:00:00Z","timestamp":1786924800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"award":["R35GM161275"],"award-info":[{"award-number":["R35GM161275"]}],"id":[{"id":"https:\/\/ror.org\/04q48ey07","id-type":"ROR","asserted-by":"publisher"}]},{"award":["R01GM111635"],"award-info":[{"award-number":["R01GM111635"]}],"id":[{"id":"https:\/\/ror.org\/04q48ey07","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Mark Groudine Chair for Outstanding Achievements in Science and Service"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,8,17]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>Injuries to individual cells happen frequently as a result of physiological and environmental stresses during their normal daily functions that can lead to a ruptured cell cortex (plasma membrane and underlying cortical cytoskeleton). The capacity of cells to rapidly repair general daily injuries, as well as ones resulting from trauma, infection, or diseases\/cancer, is essential for their survival. While we know the general cell biological outline of the highly-conserved physiological events taking place during cell wound repair, our knowledge of the molecular mechanisms governing the repair process is still fairly limited, due in large part to the lack of information regarding the molecules, machineries, and pathways involved. Here, we performed a genetic screen of 1322 fluorescent-tagged proteins to identify cell wound repair components that are recruited upon laser wounding or whose expression is lost and\/or altered upon laser wounding. We identified 129 proteins that are recruited to wounds during the cell repair process through high resolution spatio-temporal expression analyses of these gene fusions in conjunction with a fluorescent actin reporter. Strikingly, we find that many members of the Rab family GTPases are recruited to wounds where, in addition to their well-known roles in intracellular membrane trafficking, they are affecting actin cytoskeletal organization and dynamics during the repair process. These studies are allowing us to define the earliest acting proteins, as well as those required at specific steps in the repair process based on their recruitment patterns and the precise timing of their recruitment to wounds. Thus, our imaging-based screen is providing us with a global view of the repair processes, as well as a large number of genes\/gene families that provide new entry points for examining specific steps in the cell wound repair process.<\/jats:p>\n                <jats:sec>\n                  <jats:title>Author Summary<\/jats:title>\n                  <jats:p>Cells in our bodies get injured every day from normal activity, environmental stress, infection, or disease. To survive, they must quickly repair these injuries and restore normal function. While some molecules have been identified as key players of cell wound repair, many of the molecules involved and their roles remain unknown. In this study, we identified new molecules that are involved in different steps of cell wound repair. Using laser-induced injury in the Drosophila model, we examined 1322 proteins and observed their spatial and temporal dynamics in a cell after injury. From the 1322 proteins examined, we identified 129 proteins recruited to distinct regions around the damage site during cell wound repair, suggesting roles in specific steps of the repair process. Interestingly, a subset of these proteins are Rab family GTPase members, highlighting new roles for these proteins in regulating actin dynamics. By identifying new candidate repair molecules, we provide a foundation for understanding how cells maintain their integrity and how repair processes may be influenced by factors such as wound size, infection, aging, and disease.<\/jats:p>\n                <\/jats:sec>","DOI":"10.64898\/2026.08.14.744976","type":"posted-content","created":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:20:19Z","timestamp":1786976419000},"source":"Crossref","is-referenced-by-count":0,"title":["Spatiotemporal Dynamics of Protein Recruitment During Cell Wound Repair"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7879-3176","authenticated-orcid":false,"given":"Mitsutoshi","family":"Nakamura","sequence":"first","affiliation":[{"name":"Basic Sciences Division, Fred Hutchinson Cancer Center, Seattle, WA, 98109, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4123-9670","authenticated-orcid":false,"given":"Justin","family":"Hui","sequence":"additional","affiliation":[{"name":"Basic Sciences Division, Fred Hutchinson Cancer Center, Seattle, WA, 98109, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4454-6043","authenticated-orcid":false,"given":"Jeffrey M.","family":"Verboon","sequence":"additional","affiliation":[{"name":"Basic Sciences Division, Fred Hutchinson Cancer Center, Seattle, WA, 98109, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5806-9930","authenticated-orcid":false,"given":"Susan M.","family":"Parkhurst","sequence":"additional","affiliation":[{"name":"Basic Sciences Division, Fred Hutchinson Cancer Center, Seattle, WA, 98109, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026081921351066000_2026.08.14.744976v1.1","doi-asserted-by":"publisher","DOI":"10.1186\/s12915-021-00972-y"},{"key":"2026081921351066000_2026.08.14.744976v1.2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2017.12.034"},{"key":"2026081921351066000_2026.08.14.744976v1.3","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00037.2014"},{"key":"2026081921351066000_2026.08.14.744976v1.4","doi-asserted-by":"publisher","DOI":"10.3390\/ijms221910886"},{"key":"2026081921351066000_2026.08.14.744976v1.5","doi-asserted-by":"crossref","unstructured":"Nakamura M , Dominguez ANM , Decker JR , Hull AJ , Verboon JM , Parkhurst SM . 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