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During early brain development, dysfunction of the primary cilia can lead to a broad spectrum of disorders, collectively termed ciliopathies, that include brain malformations and intellectual disability. Although the role of primary cilia in brain development is well-established, cilia-mediated signaling in mature neurons and the contribution of cilia to neuronal circuit function remain controversial. Using mouse genetic and behavioral studies, single-cell electrophysiology, and 2-photon imaging, we show that deletion of primary cilia from adult hippocampal neurons is not required for hippocampal circuit function or behavior. Chronic genetic deletion or acute laser ablation of primary cilia from mature pyramidal neurons in the CA1 or CA3 regions of the hippocampus did not affect neuronal excitability, basal synaptic transmission, or long-term synaptic plasticity at excitatory CA3\u2013CA1 hippocampal synapses. Moreover, the loss of primary cilia did not affect hippocampal-dependent learning and memory or anxiety-like behaviors. These results challenge the prevailing view of cilia function in mature hippocampal neurons and suggest that neuronal cilia in the adult hippocampus do not serve as major signaling hubs for pathways essential for neuronal function or behavior.<\/jats:p>","DOI":"10.64898\/2026.08.24.746770","type":"posted-content","created":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T01:10:27Z","timestamp":1787879427000},"source":"Crossref","is-referenced-by-count":0,"title":["Neuronal primary cilia are not required for hippocampal circuit function or behavior in adult mice"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7049-9079","authenticated-orcid":false,"given":"Tae-Yeon","family":"Eom","sequence":"first","affiliation":[{"name":"Division of Neural Circuits and Behavior, Department of Developmental Neurobiology, St. Jude Children\u2019s Research Hospital, Memphis, TN 38105, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ildar T.","family":"Bayazitov","sequence":"additional","affiliation":[{"name":"Division of Neural Circuits and Behavior, Department of Developmental Neurobiology, St. Jude Children\u2019s Research Hospital, Memphis, TN 38105, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brett J.W.","family":"Teubner","sequence":"additional","affiliation":[{"name":"Division of Neural Circuits and Behavior, Department of Developmental Neurobiology, St. Jude Children\u2019s Research Hospital, Memphis, TN 38105, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donnie","family":"Eddins","sequence":"additional","affiliation":[{"name":"Division of Neural Circuits and Behavior, Department of Developmental Neurobiology, St. Jude Children\u2019s Research Hospital, Memphis, TN 38105, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8115-202X","authenticated-orcid":false,"given":"Stanislav S.","family":"Zakharenko","sequence":"additional","affiliation":[{"name":"Division of Neural Circuits and Behavior, Department of Developmental Neurobiology, St. Jude Children\u2019s Research Hospital, Memphis, TN 38105, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026100108000504000_2026.08.24.746770v2.1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2017.08.031"},{"key":"2026100108000504000_2026.08.24.746770v2.2","doi-asserted-by":"publisher","DOI":"10.1038\/nature02061"},{"key":"2026100108000504000_2026.08.24.746770v2.3","doi-asserted-by":"publisher","DOI":"10.1038\/nature02030"},{"key":"2026100108000504000_2026.08.24.746770v2.4","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2014.04.024"},{"key":"2026100108000504000_2026.08.24.746770v2.5","doi-asserted-by":"publisher","DOI":"10.1038\/s41576-023-00587-9"},{"key":"2026100108000504000_2026.08.24.746770v2.6","doi-asserted-by":"publisher","DOI":"10.1016\/S0070-2153(08)00802-8"},{"key":"2026100108000504000_2026.08.24.746770v2.7","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2774"},{"key":"2026100108000504000_2026.08.24.746770v2.8","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2009.05.025"},{"key":"2026100108000504000_2026.08.24.746770v2.9","doi-asserted-by":"publisher","DOI":"10.1016\/j.gde.2009.04.008"},{"key":"2026100108000504000_2026.08.24.746770v2.10","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1010172"},{"key":"2026100108000504000_2026.08.24.746770v2.11","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a028191"},{"key":"2026100108000504000_2026.08.24.746770v2.12","doi-asserted-by":"publisher","DOI":"10.1038\/nn.3042"},{"key":"2026100108000504000_2026.08.24.746770v2.13","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5586-06.2007"},{"key":"2026100108000504000_2026.08.24.746770v2.14","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2012.09.019"},{"key":"2026100108000504000_2026.08.24.746770v2.15","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1321425111"},{"key":"2026100108000504000_2026.08.24.746770v2.16","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0106576"},{"key":"2026100108000504000_2026.08.24.746770v2.17","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5295-09.2010"},{"key":"2026100108000504000_2026.08.24.746770v2.18","doi-asserted-by":"crossref","first-page":"102617","DOI":"10.1016\/j.isci.2021.102617","article-title":"Primary cilia are required for the persistence of memory and stabilization of perineuronal nets","volume":"24","year":"2021","journal-title":"iScience"},{"key":"2026100108000504000_2026.08.24.746770v2.19","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1038\/s41598-024-83432-1","article-title":"Temporal ablation of the ciliary protein IFT88 alters normal brainwave patterns","volume":"15","year":"2025","journal-title":"Sci. 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