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Transferred mitochondria were depolarized and accumulated reactive oxygen species (ROS), and the mitochondrial transfer-induced proliferation was dependent upon ROS signaling (Kidwell et al. 2023). Our unexpected findings supported a model in which transferred mitochondria act as a signal for proliferation in acceptor cancer cells rather than a direct source of increased bioenergetics. It remains unclear whether this unexpected signaling mechanism is unique to macrophages as the donor cell, or whether this mechanism applies to mitochondrial transfer between other cells within the tumor microenvironment. Here, we show that highly metastatic cancer cells transfer mitochondria to weakly metastatic cancer cells. These transferred mitochondria are depolarized, accumulate ROS, and promote ROS-dependent proliferation in acceptor cancer cells. Furthermore, we specifically attribute this proliferative phenotype to the transfer of mitochondria, as when we isolate mitochondria from highly metastatic cells and apply these purified mitochondria directly to weakly metastatic cells in culture, acceptor cancer cells that internalize the purified mitochondria exhibit increased proliferation in a ROS-dependent manner. These findings support mitochondrial transfer within the breast tumor microenvironment as a signaling axis for proliferation, regardless of donor cell identity.<\/jats:p>","DOI":"10.64898\/2026.08.26.747325","type":"posted-content","created":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T14:20:18Z","timestamp":1787840418000},"source":"Crossref","is-referenced-by-count":0,"title":["Mitochondrial transfer between breast cancer cells promotes ROS-dependent proliferation"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-3532-2624","authenticated-orcid":false,"given":"Noah M.","family":"Bressler","sequence":"first","affiliation":[{"name":"Department of Biochemistry, University of Utah School of Medicine; Salt Lake City, UT, 84112, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taylor Z.","family":"Stevens","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Utah School of Medicine; Salt Lake City, UT, 84112, USA"},{"name":"Molecular Plant Sciences Program, Washington State University, Pullman, WA, 99164, USA, University of Utah; Salt Lake City, UT, 84112, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3961-4547","authenticated-orcid":false,"given":"Minna","family":"Roh-Johnson","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Utah School of Medicine; Salt Lake City, UT, 84112, USA"},{"name":"Huntsman Cancer Institute, University of Utah; Salt Lake City, UT, 84112, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026083108150970000_2026.08.26.747325v1.1","doi-asserted-by":"publisher","DOI":"10.1002\/embj.201386030"},{"key":"2026083108150970000_2026.08.26.747325v1.2","doi-asserted-by":"publisher","DOI":"10.1038\/s41588-019-0507-7"},{"key":"2026083108150970000_2026.08.26.747325v1.3","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2012.81"},{"key":"2026083108150970000_2026.08.26.747325v1.4","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2024.08.029"},{"key":"2026083108150970000_2026.08.26.747325v1.5","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2018.03.003"},{"key":"2026083108150970000_2026.08.26.747325v1.6","doi-asserted-by":"crossref","first-page":"1714","DOI":"10.1038\/s43018-025-01038-6","article-title":"MIRO2-mediated mitochondrial transfer from cancer cells induces cancer-associated fibroblast differentiation","volume":"6","year":"2025","journal-title":"Nat Cancer"},{"key":"2026083108150970000_2026.08.26.747325v1.7","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-77-18779"},{"key":"2026083108150970000_2026.08.26.747325v1.8","doi-asserted-by":"publisher","DOI":"10.1038\/1731094a0"},{"key":"2026083108150970000_2026.08.26.747325v1.9","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0032778"},{"key":"2026083108150970000_2026.08.26.747325v1.10","doi-asserted-by":"crossref","first-page":"6471","DOI":"10.7150\/thno.96723","article-title":"Mitochondrial transfer balances cell redox, energy and metabolic homeostasis in the osteoarthritic chondrocyte preserving cartilage integrity","volume":"14","year":"2024","journal-title":"Theranostics"},{"key":"2026083108150970000_2026.08.26.747325v1.11","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-17813-0"},{"key":"2026083108150970000_2026.08.26.747325v1.12","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2021.08.002"},{"key":"2026083108150970000_2026.08.26.747325v1.13","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1404651111"},{"key":"2026083108150970000_2026.08.26.747325v1.14","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1186\/s13046-024-03087-8","article-title":"Mitochondrial transfer from Adipose stem cells to breast cancer cells drives multi-drug resistance","volume":"43","year":"2024","journal-title":"J Exp Clin Cancer Res"},{"key":"2026083108150970000_2026.08.26.747325v1.15","doi-asserted-by":"crossref","unstructured":"Dong, L. 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