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However, crowding-induced extrusion does not necessarily remove damaged or otherwise unfit cells. Here, we show that glucose or glutamine starvation triggers a rapid, regulated wave of extrusion, called starvation-induced cell extrusion (STICE), that selectively eliminates cells bearing DNA damage markers via a p53-dependent, Piezo1-independent pathway, improving monolayer fitness. Unlike non-extruding cells, which recycle contents through autophagy and lysosomal digestion, p53-activated cells instead use LC3 to drive lysosomal exocytosis, promoting extrusion signalling. By eliminating defective and transformed cells, STICE confers resistance to damage and apoptotic stimuli in the remaining monolayer. STICE thus acts as a tissue-level analogue of autophagy: rather than improving individual cells by digesting and recycling damaged components, it improves tissue fitness by eliminating substandard cells.<\/jats:p>","DOI":"10.64898\/2026.09.01.748489","type":"posted-content","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T07:25:12Z","timestamp":1788420312000},"source":"Crossref","is-referenced-by-count":0,"title":["Starvation improves epithelial fitness by selectively extruding DNA damaged cells"],"prefix":"10.64898","author":[{"given":"Lily A.","family":"Gates","sequence":"first","affiliation":[{"name":"The Randall Centre for Cell & Molecular Biophysics, School of Basic & Medical Biosciences, King\u2019s College London, London, UK"},{"name":"The Francis Crick Institute, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0450-3727","authenticated-orcid":false,"given":"Dustin C.","family":"Bagley","sequence":"additional","affiliation":[{"name":"The Randall Centre for Cell & Molecular Biophysics, School of Basic & Medical Biosciences, King\u2019s College London, London, UK"},{"name":"The Francis Crick Institute, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7640-8905","authenticated-orcid":false,"given":"Konstantinos","family":"Kalyviotis","sequence":"additional","affiliation":[{"name":"The Francis Crick Institute, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8944-0664","authenticated-orcid":false,"given":"John","family":"Fadul","sequence":"additional","affiliation":[{"name":"The Francis Crick Institute, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7748-2939","authenticated-orcid":false,"given":"Tania","family":"Auchynnikava","sequence":"additional","affiliation":[{"name":"The Francis Crick Institute, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5636-9892","authenticated-orcid":false,"given":"Maria","family":"Voronkov","sequence":"additional","affiliation":[{"name":"The Francis Crick Institute, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9460-5868","authenticated-orcid":false,"given":"Jody","family":"Rosenblatt","sequence":"additional","affiliation":[{"name":"The Randall Centre for Cell & Molecular Biophysics, School of Basic & Medical Biosciences, King\u2019s College London, London, UK"},{"name":"The Francis Crick Institute, London, UK"},{"name":"Comprehensive Cancer Centre, School of Cancer & Pharmaceutical Sciences, King\u2019s College London, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026092709100525000_2026.09.01.748489v2.1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10999"},{"key":"2026092709100525000_2026.09.01.748489v2.2","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2023.03.008"},{"key":"2026092709100525000_2026.09.01.748489v2.3","doi-asserted-by":"crossref","unstructured":"Rosenblatt, J. , Raff, M. 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