{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T18:08:46Z","timestamp":1789063726600,"version":"build-2803163510"},"posted":{"date-parts":[[2026,9,8]]},"group-title":"Biophysics","reference-count":100,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:00:00Z","timestamp":1788825600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"award":["GM144581"],"award-info":[{"award-number":["GM144581"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]},{"id":[{"id":"https:\/\/ror.org\/0210rze73","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,9,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>\n                  The function and gating mechanism of\n                  <jats:italic>Escherichia coli<\/jats:italic>\n                  mechanosensitive channel of small conductance (\n                  <jats:italic>Ec<\/jats:italic>\n                  MscS) is well understood, but it is unknown whether MscS homologs in other bacteria function the same way. Here, we show that the MscS homolog from\n                  <jats:italic>Corynebacterium glutamicum<\/jats:italic>\n                  (\n                  <jats:italic>Cg<\/jats:italic>\n                  MscS) opens at a much higher membrane tension than\n                  <jats:italic>Ec<\/jats:italic>\n                  MscS but has otherwise similar functional characteristics.\n                  <jats:italic>Cg<\/jats:italic>\n                  MscS is also structurally similar to\n                  <jats:italic>Ec<\/jats:italic>\n                  MscS but features an extended transmembrane (TM) helix 2 that forms salt bridges with the cytoplasmic cage. Compared to the closed conformation, the TM1-2 sensor paddles in the inactivated conformation are rotated but not tilted, and, unlike\n                  <jats:italic>Ec<\/jats:italic>\n                  MscS, there is no change in the associated pocket lipids, thus establishing a gating mechanism distinct from\n                  <jats:italic>Ec<\/jats:italic>\n                  MscS that is not based on the \u201clipids-move-first\u201d model. Because the TM2 extension is conserved in Actinobacteria but not other bacterial phyla, our findings suggest that MscS homologs have lineage-specific gating mechanisms.\n                <\/jats:p>","DOI":"10.64898\/2026.09.02.748947","type":"posted-content","created":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T22:20:11Z","timestamp":1788906011000},"source":"Crossref","is-referenced-by-count":0,"title":["The MscS channel from\n                  <i>Corynebacterium glutamicum<\/i>\n                  uses a non-canonical gating mechanism"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6797-5556","authenticated-orcid":false,"given":"Yoshitaka","family":"Nakayama","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Electron Microscopy, The Rockefeller University; New York, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8731-7427","authenticated-orcid":false,"given":"Giorgos","family":"Hiotis","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Electron Microscopy, The Rockefeller University; New York, NY, USA"},{"name":"Tri-Institutional PhD Program in Chemical Biology, The Rockefeller University; New York, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2606-2835","authenticated-orcid":false,"given":"Thomas","family":"Walz","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Electron Microscopy, The Rockefeller University; New York, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026091010400648000_2026.09.02.748947v1.1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2933-1"},{"key":"2026091010400648000_2026.09.02.748947v1.2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(93)81044-0"},{"key":"2026091010400648000_2026.09.02.748947v1.3","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.84.8.2297"},{"key":"2026091010400648000_2026.09.02.748947v1.4","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(02)75169-2"},{"key":"2026091010400648000_2026.09.02.748947v1.5","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1200051109"},{"key":"2026091010400648000_2026.09.02.748947v1.6","doi-asserted-by":"publisher","DOI":"10.1038\/368265a0"},{"key":"2026091010400648000_2026.09.02.748947v1.7","doi-asserted-by":"publisher","DOI":"10.1038\/348261a0"},{"key":"2026091010400648000_2026.09.02.748947v1.8","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2023.12.007"},{"key":"2026091010400648000_2026.09.02.748947v1.9","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.200910371"},{"key":"2026091010400648000_2026.09.02.748947v1.10","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms3137"},{"key":"2026091010400648000_2026.09.02.748947v1.11","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb1341"},{"key":"2026091010400648000_2026.09.02.748947v1.12","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.107.123448"},{"key":"2026091010400648000_2026.09.02.748947v1.13","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb895"},{"key":"2026091010400648000_2026.09.02.748947v1.14","doi-asserted-by":"publisher","DOI":"10.1126\/science.1077945"},{"key":"2026091010400648000_2026.09.02.748947v1.15","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-440X(03)00106-4"},{"key":"2026091010400648000_2026.09.02.748947v1.16","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.104.046045"},{"key":"2026091010400648000_2026.09.02.748947v1.17","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(04)74340-4"},{"key":"2026091010400648000_2026.09.02.748947v1.18","doi-asserted-by":"publisher","DOI":"10.1126\/science.1159262"},{"key":"2026091010400648000_2026.09.02.748947v1.19","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2107095118"},{"key":"2026091010400648000_2026.09.02.748947v1.20","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2019.07.006"},{"key":"2026091010400648000_2026.09.02.748947v1.21","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.50486"},{"key":"2026091010400648000_2026.09.02.748947v1.22","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03196-w"},{"key":"2026091010400648000_2026.09.02.748947v1.23","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.3120"},{"key":"2026091010400648000_2026.09.02.748947v1.24","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.7.1730"},{"key":"2026091010400648000_2026.09.02.748947v1.25","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.201611699"},{"key":"2026091010400648000_2026.09.02.748947v1.26","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2024.110001"},{"key":"2026091010400648000_2026.09.02.748947v1.27","doi-asserted-by":"publisher","DOI":"10.1111\/1751-7915.70252"},{"key":"2026091010400648000_2026.09.02.748947v1.28","doi-asserted-by":"publisher","DOI":"10.1385\/CBB:34:3:349"},{"key":"2026091010400648000_2026.09.02.748947v1.29","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms2014"},{"key":"2026091010400648000_2026.09.02.748947v1.30","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2005.11.044"},{"key":"2026091010400648000_2026.09.02.748947v1.31","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-28511-5"},{"key":"2026091010400648000_2026.09.02.748947v1.32","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-63419-w"},{"key":"2026091010400648000_2026.09.02.748947v1.33","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.67449"},{"key":"2026091010400648000_2026.09.02.748947v1.34","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.67.1.66-85.2003"},{"key":"2026091010400648000_2026.09.02.748947v1.35","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201200410"},{"key":"2026091010400648000_2026.09.02.748947v1.36","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0301951"},{"key":"2026091010400648000_2026.09.02.748947v1.37","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-62757-z"},{"key":"2026091010400648000_2026.09.02.748947v1.38","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-34737-0"},{"key":"2026091010400648000_2026.09.02.748947v1.39","doi-asserted-by":"publisher","DOI":"10.3390\/ijms232315071"},{"key":"2026091010400648000_2026.09.02.748947v1.40","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-63253-0"},{"key":"2026091010400648000_2026.09.02.748947v1.41","doi-asserted-by":"publisher","DOI":"10.4161\/chan.20998"},{"key":"2026091010400648000_2026.09.02.748947v1.42","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-62805-8"},{"key":"2026091010400648000_2026.09.02.748947v1.43","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-014-1522-2"},{"key":"2026091010400648000_2026.09.02.748947v1.44","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02446-06"},{"key":"2026091010400648000_2026.09.02.748947v1.45","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02691-17"},{"key":"2026091010400648000_2026.09.02.748947v1.46","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2010.06.022"},{"key":"2026091010400648000_2026.09.02.748947v1.47","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-31219-6"},{"key":"2026091010400648000_2026.09.02.748947v1.48","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01310-12"},{"key":"2026091010400648000_2026.09.02.748947v1.49","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2013.01.001"},{"key":"2026091010400648000_2026.09.02.748947v1.50","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2020.05.005"},{"key":"2026091010400648000_2026.09.02.748947v1.51","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.1c07086"},{"key":"2026091010400648000_2026.09.02.748947v1.52","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-025-02654-4"},{"key":"2026091010400648000_2026.09.02.748947v1.53","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.202400540"},{"key":"2026091010400648000_2026.09.02.748947v1.54","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00986-07"},{"key":"2026091010400648000_2026.09.02.748947v1.55","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2020.102015"},{"key":"2026091010400648000_2026.09.02.748947v1.56","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.1775"},{"key":"2026091010400648000_2026.09.02.748947v1.57","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2013.06.054"},{"key":"2026091010400648000_2026.09.02.748947v1.58","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1001405107"},{"key":"2026091010400648000_2026.09.02.748947v1.59","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms11010194"},{"key":"2026091010400648000_2026.09.02.748947v1.60","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.201110606"},{"key":"2026091010400648000_2026.09.02.748947v1.61","doi-asserted-by":"publisher","DOI":"10.1096\/fj.15-275198"},{"key":"2026091010400648000_2026.09.02.748947v1.62","doi-asserted-by":"publisher","DOI":"10.1016\/S0263-7855(97)00009-X"},{"key":"2026091010400648000_2026.09.02.748947v1.63","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-63253-0"},{"key":"2026091010400648000_2026.09.02.748947v1.64","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2104820118"},{"key":"2026091010400648000_2026.09.02.748947v1.65","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-026-75798-9"},{"key":"2026091010400648000_2026.09.02.748947v1.66","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.113.4.525"},{"key":"2026091010400648000_2026.09.02.748947v1.67","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.31.18329"},{"key":"2026091010400648000_2026.09.02.748947v1.68","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2004.07.045"},{"key":"2026091010400648000_2026.09.02.748947v1.69","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07487-w"},{"key":"2026091010400648000_2026.09.02.748947v1.70","doi-asserted-by":"publisher","DOI":"10.1007\/s00249-015-1041-x"},{"key":"2026091010400648000_2026.09.02.748947v1.71","doi-asserted-by":"publisher","DOI":"10.1002\/bit.24954"},{"key":"2026091010400648000_2026.09.02.748947v1.72","doi-asserted-by":"publisher","DOI":"10.1251\/bpo70"},{"key":"2026091010400648000_2026.09.02.748947v1.73","doi-asserted-by":"publisher","DOI":"10.1002\/pro.3967"},{"key":"2026091010400648000_2026.09.02.748947v1.74","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsb.2005.07.007"},{"key":"2026091010400648000_2026.09.02.748947v1.75","doi-asserted-by":"publisher","DOI":"10.1002\/pro.4792"},{"key":"2026091010400648000_2026.09.02.748947v1.76","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444910007493"},{"key":"2026091010400648000_2026.09.02.748947v1.77","doi-asserted-by":"publisher","DOI":"10.1107\/S2059798318006551"},{"key":"2026091010400648000_2026.09.02.748947v1.78","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-021-02399-1"},{"key":"2026091010400648000_2026.09.02.748947v1.79","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.20945"},{"key":"2026091010400648000_2026.09.02.748947v1.80","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jctc.5b00935"},{"key":"2026091010400648000_2026.09.02.748947v1.81","doi-asserted-by":"publisher","DOI":"10.1021\/j100059a038"},{"key":"2026091010400648000_2026.09.02.748947v1.82","doi-asserted-by":"publisher","DOI":"10.1063\/1.445869"},{"key":"2026091010400648000_2026.09.02.748947v1.83","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.540150702"},{"key":"2026091010400648000_2026.09.02.748947v1.84","doi-asserted-by":"publisher","DOI":"10.1063\/1.464397"},{"key":"2026091010400648000_2026.09.02.748947v1.85","doi-asserted-by":"publisher","DOI":"10.1063\/1.470117"},{"key":"2026091010400648000_2026.09.02.748947v1.86","doi-asserted-by":"publisher","DOI":"10.1063\/5.0020514"},{"key":"2026091010400648000_2026.09.02.748947v1.87","doi-asserted-by":"publisher","DOI":"10.1063\/1.2408420"},{"key":"2026091010400648000_2026.09.02.748947v1.88","doi-asserted-by":"publisher","DOI":"10.1021\/ct700200b"},{"key":"2026091010400648000_2026.09.02.748947v1.89","doi-asserted-by":"publisher","DOI":"10.1016\/j.softx.2015.06.001"},{"key":"2026091010400648000_2026.09.02.748947v1.90","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4067"},{"key":"2026091010400648000_2026.09.02.748947v1.91","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.23354"},{"key":"2026091010400648000_2026.09.02.748947v1.92","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.108.136499"},{"key":"2026091010400648000_2026.09.02.748947v1.93","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.21787"},{"key":"2026091010400648000_2026.09.02.748947v1.94","doi-asserted-by":"publisher","DOI":"10.25080\/Majora-629e541a-00e"},{"key":"2026091010400648000_2026.09.02.748947v1.95","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/mst010"},{"key":"2026091010400648000_2026.09.02.748947v1.96","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp348"},{"key":"2026091010400648000_2026.09.02.748947v1.97","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msaa015"},{"key":"2026091010400648000_2026.09.02.748947v1.98","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkae268"},{"key":"2026091010400648000_2026.09.02.748947v1.99","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp033"},{"key":"2026091010400648000_2026.09.02.748947v1.100","doi-asserted-by":"publisher","DOI":"10.1101\/gr.849004"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.64898\/2026.09.02.748947","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T17:40:16Z","timestamp":1789062016000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.64898\/2026.09.02.748947"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,8]]},"references-count":100,"URL":"https:\/\/doi.org\/10.64898\/2026.09.02.748947","relation":{},"subject":[],"published":{"date-parts":[[2026,9,8]]},"subtype":"preprint"}}