{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T16:11:05Z","timestamp":1789056665611,"version":"build-2803163510"},"posted":{"date-parts":[[2026,9,7]]},"group-title":"Biochemistry","reference-count":17,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T00:00:00Z","timestamp":1788739200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R01DK106104"],"award-info":[{"award-number":["R01DK106104"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["K01DK133667"],"award-info":[{"award-number":["K01DK133667"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000738","name":"United States Department of Veterans Affairs","doi-asserted-by":"crossref","award":["I01BX004634"],"award-info":[{"award-number":["I01BX004634"]}],"id":[{"id":"10.13039\/100000738","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,9,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Endocrine crosstalk between the liver and the brain regulates systemic energy homeostasis. However, this communication has not been comprehensively evaluated because identification of liver-derived proteins, or hepatokines, is difficult to define using bulk serum proteomics alone. To overcome this problem, we applied hepatocyte-restricted endoplasmic-reticulum\u2013targeted TurboID (ER-TurboID)\n                  <jats:italic>in vivo<\/jats:italic>\n                  and recovered biotinylated, liver-secretion\u2013competent proteins directly from specific brain regions, including the hypothalamus and hindbrain (nucleus of the solitary tract (NTS) and area postrema (AP)). Compared with AAV8-Null transduced controls, TurboID-expressing animals showed 3-to 21-fold enrichment of liver-expressed and predicted liver-secreted proteins among brain regions, with 81 canonical liver-secretome proteins, including multiple carboxylesterase (CES) family members. These results establish a method to identify novel secreted factors from peripheral tissues that can potentially act on specific brain regions.\n                <\/jats:p>","DOI":"10.64898\/2026.09.03.749208","type":"posted-content","created":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T20:55:14Z","timestamp":1788814514000},"source":"Crossref","is-referenced-by-count":0,"title":["Method to Identify Liver-to-Brain Region-Specific Crosstalk Using TurboID"],"prefix":"10.64898","author":[{"given":"Matthew C.","family":"Juber","sequence":"first","affiliation":[{"name":"Department of Neuroscience and Pharmacology, University of Iowa Carver College of Medicine, Iowa City, IA 52242, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arvand","family":"Asghari","sequence":"additional","affiliation":[{"name":"Department of Neuroscience and Pharmacology, University of Iowa Carver College of Medicine, Iowa City, IA 52242, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristin E.","family":"Claflin","sequence":"additional","affiliation":[{"name":"Department of Neuroscience and Pharmacology, University of Iowa Carver College of Medicine, Iowa City, IA 52242, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9158-5128","authenticated-orcid":false,"given":"Matthew J.","family":"Potthoff","sequence":"additional","affiliation":[{"name":"Department of Neuroscience and Pharmacology, University of Iowa Carver College of Medicine, Iowa City, IA 52242, USA"},{"name":"Harold Hamm Diabetes Center, University of Oklahoma Health Sciences, Oklahoma City, OK 73104, USA"},{"name":"Department of Biochemistry and Physiology, University of Oklahoma Health Sciences, Oklahoma City, OK 73104, USA"},{"name":"Department of Veterans Affairs Medical Center, Oklahoma City, OK 73104, 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