{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T19:07:03Z","timestamp":1789153623896,"version":"build-2803163510"},"posted":{"date-parts":[[2026,9,8]]},"group-title":"Neuroscience","reference-count":21,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T00:00:00Z","timestamp":1788825600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R01 DK134431"],"award-info":[{"award-number":["R01 DK134431"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R01 DK119183"],"award-info":[{"award-number":["R01 DK119183"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R01 DK138907"],"award-info":[{"award-number":["R01 DK138907"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["1S10OD028596"],"award-info":[{"award-number":["1S10OD028596"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["U54 DK137329"],"award-info":[{"award-number":["U54 DK137329"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,9,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Sensory neurons that innervate the urinary bladder are critical for triggering voiding and mediating pain perception under pathological conditions. Despite functional and immunohistochemical evidence indicating the existence of several bladder afferent populations, the subtypes of dorsal root ganglion (DRG) neurons that innervate this organ are poorly defined and specific markers to study them have not been described. Here, we combined retrograde tracing with the cholera toxin \u03b2 subunit and immunofluorescence\n                  <jats:italic>in situ<\/jats:italic>\n                  hybridization with markers defined by transcriptomic analysis of C and A\u03b4 afferents to classify the lumbosacral DRG neurons that innervate the urinary bladder. Four lumbosacral (L6-S2) bladder-innervating DRG neuron populations were identified: three\n                  <jats:italic>Calca<\/jats:italic>\n                  <jats:sup>+<\/jats:sup>\n                  peptidergic populations that express\n                  <jats:italic>Adra2a<\/jats:italic>\n                  \/\n                  <jats:italic>Th<\/jats:italic>\n                  ,\n                  <jats:italic>Th<\/jats:italic>\n                  \/\n                  <jats:italic>Ntrk2<\/jats:italic>\n                  , or\n                  <jats:italic>Ntrk2<\/jats:italic>\n                  , and one non-peptidergic population that expresses\n                  <jats:italic>Ntrk2<\/jats:italic>\n                  . Among the whole population of peptidergic lumbosacral sensory neurons,\n                  <jats:italic>Adra2a<\/jats:italic>\n                  <jats:sup>+<\/jats:sup>\n                  \/\n                  <jats:italic>Th<\/jats:italic>\n                  <jats:sup>+<\/jats:sup>\n                  ,\n                  <jats:italic>\n                    Th\n                    <jats:sup>+<\/jats:sup>\n                  <\/jats:italic>\n                  \/\n                  <jats:italic>Ntrk2<\/jats:italic>\n                  <jats:sup>+<\/jats:sup>\n                  and\n                  <jats:italic>Ntrk2<\/jats:italic>\n                  <jats:sup>+<\/jats:sup>\n                  neurons were rare. Thus, our studies identified multiple populations of bladder-innervating DRG neurons, revealing unappreciated diversity among the sensory neuron subtypes present at the lumbosacral level that innervate visceral and somatic tissues.\n                <\/jats:p>","DOI":"10.64898\/2026.09.03.749247","type":"posted-content","created":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T07:30:17Z","timestamp":1788939017000},"source":"Crossref","is-referenced-by-count":0,"title":["Subtypes of Bladder-Innervating Sensory Neurons revealed by Immuno-Fluorescence In Situ Hybridization"],"prefix":"10.64898","author":[{"given":"Xuejiao","family":"Sun","sequence":"first","affiliation":[{"name":"Renal-Electrolyte Division, Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guadalupe","family":"Manrique-Maldonado","sequence":"additional","affiliation":[{"name":"Renal-Electrolyte Division, Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1901-1618","authenticated-orcid":false,"given":"Marcelo D.","family":"Carattino","sequence":"additional","affiliation":[{"name":"Renal-Electrolyte Division, Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania"},{"name":"Department of Cell Biology, University of Pittsburgh, Pittsburgh, Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026091111202132000_2026.09.03.749247v1.1","doi-asserted-by":"publisher","DOI":"10.1111\/apha.12014"},{"key":"2026091111202132000_2026.09.03.749247v1.2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-540-79090-7_4"},{"key":"2026091111202132000_2026.09.03.749247v1.3","doi-asserted-by":"publisher","DOI":"10.1038\/nn.3881"},{"key":"2026091111202132000_2026.09.03.749247v1.4","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-36014-0"},{"key":"2026091111202132000_2026.09.03.749247v1.5","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2019.05.039"},{"key":"2026091111202132000_2026.09.03.749247v1.6","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-1900-1"},{"key":"2026091111202132000_2026.09.03.749247v1.7","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2024.02.006"},{"key":"2026091111202132000_2026.09.03.749247v1.8","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2017-315631"},{"key":"2026091111202132000_2026.09.03.749247v1.9","doi-asserted-by":"publisher","DOI":"10.1097\/j.pain.0000000000002831"},{"key":"2026091111202132000_2026.09.03.749247v1.10","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1993.sp019604"},{"key":"2026091111202132000_2026.09.03.749247v1.11","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6123(08)62758-2"},{"key":"2026091111202132000_2026.09.03.749247v1.12","doi-asserted-by":"publisher","DOI":"10.1007\/BF00657509"},{"key":"2026091111202132000_2026.09.03.749247v1.13","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.1976.sp011476"},{"key":"2026091111202132000_2026.09.03.749247v1.14","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3959(96)02993-4"},{"key":"2026091111202132000_2026.09.03.749247v1.15","doi-asserted-by":"publisher","DOI":"10.1152\/jn.1994.72.5.2420"},{"issue":"6","key":"2026091111202132000_2026.09.03.749247v1.16","doi-asserted-by":"crossref","first-page":"2046","DOI":"10.1152\/jn.1994.71.6.2046","article-title":"Characterization of mechanosensitive pelvic nerve afferent fibers innervating the colon of the rat","volume":"71","year":"1994","journal-title":"J Neurophysiol"},{"key":"2026091111202132000_2026.09.03.749247v1.17","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0189941"},{"key":"2026091111202132000_2026.09.03.749247v1.18","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2008.10.139"},{"key":"2026091111202132000_2026.09.03.749247v1.19","doi-asserted-by":"publisher","DOI":"10.1111\/iju.13181"},{"key":"2026091111202132000_2026.09.03.749247v1.20","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2023.07.007"},{"key":"2026091111202132000_2026.09.03.749247v1.21","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5239-04.2005"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.64898\/2026.09.03.749247","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T18:20:31Z","timestamp":1789150831000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.64898\/2026.09.03.749247"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,8]]},"references-count":21,"URL":"https:\/\/doi.org\/10.64898\/2026.09.03.749247","relation":{},"subject":[],"published":{"date-parts":[[2026,9,8]]},"subtype":"preprint"}}