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In this work, we establish an approach for creating self-assembling, supramolecular hydrogels based on a peptide-polymer conjugate (PPC) as a three-dimensional (3D) cell culture platform. The viscoelastic properties of these hydrogels, including thermoresponsiveness and shear-thinning, were measured using rheometry. Building on these observations, we then developed a method for the encapsulation and culture of human wound healing cells, fibroblasts, within the PPC hydrogels, observing both excellent cell viability and material stability over time. The modulation of stiffness by temperature variation and the reversible nature of these hydrogels imparts unique properties for both cell encapsulation and harvesting and offers potential for a variety of biomedical applications, from 3D cell culture systems to therapeutic delivery.<\/jats:p>\n                <jats:sec>\n                  <jats:title>Abstract Figure<\/jats:title>\n                  <jats:fig id=\"ufig1\" position=\"float\" orientation=\"portrait\" fig-type=\"figure\">\n                    <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"749227v1_ufig1\" position=\"float\" orientation=\"portrait\"\/>\n                  <\/jats:fig>\n                <\/jats:sec>","DOI":"10.64898\/2026.09.04.749227","type":"posted-content","created":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T12:10:16Z","timestamp":1788955816000},"source":"Crossref","is-referenced-by-count":0,"title":["Thermoresponsive, Shear-Thinning Supramolecular Hydrogels from Peptide\u2013Polymer Conjugates for Bioinspired 3D Cell Culture"],"prefix":"10.64898","author":[{"given":"Breanna M.","family":"Huntington","sequence":"first","affiliation":[{"name":"Department of Chemical and Biological Engineering, University of Delaware, Newark, DE"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes A.","family":"Dresel","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Johannes Gutenberg-Universit\u00e4t Mainz, Mainz, Germany"},{"name":"Department of Materials Science and Engineering, University of Delaware, Newark, DE"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maren","family":"Schweitzer","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Johannes Gutenberg-Universit\u00e4t Mainz, Mainz, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric M.","family":"Furst","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biological Engineering, University of Delaware, Newark, DE"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pol","family":"Besenius","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Johannes Gutenberg-Universit\u00e4t Mainz, Mainz, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"April M.","family":"Kloxin","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biological Engineering, University of Delaware, Newark, DE"},{"name":"Department of Materials Science and Engineering, University of Delaware, Newark, DE"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026091206001158000_2026.09.04.749227v1.1","doi-asserted-by":"crossref","unstructured":"Tang, S. ; Zhao, L. ; Yuan, J. ; Chen, Y. ; Leng, Y . 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