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Soft tissue biomechanics is heavily influenced by tissue water content; therefore, accurately assessing water content is needed to describe iris biomechanics in a physiologically relevant manner. Yet, accurate experimental measurements of iris water content are rare in the literature. Therefore, our objective was to experimentally assess iris water content and potential sex differences in New Zealand White rabbit iris\u2014a suitable model for studying iris biomechanics. We also examined the effect of exposure to phosphate-buffered saline (PBS) on iris water content, given its common use to maintain hydration during soft-tissue biomechanical testing. Our results showed slightly higher water content in males (89.2% \u00b1 2.3%) than in females (87.7% \u00b1 2.2%) (p = 0.022), while we found no significant differences in gross iris morphology between the sexes, indicating subtle compositional differences. Furthermore, PBS incubation had a minimal effect on water content only in female irides. Overall, our results indicated high iris water content in both sexes compared with anatomically adjacent ocular tissues and other soft connective tissues. These results improve our understanding of iris composition and physiology and can serve as a baseline for constructing biomechanical models to assess its role in PACG.<\/jats:p>","DOI":"10.64898\/2026.09.04.749502","type":"posted-content","created":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T22:05:21Z","timestamp":1789164321000},"source":"Crossref","is-referenced-by-count":0,"title":["Experimental Assessment of Iris Water Content and Sex Differences in Mature Rabbits"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-5598-1714","authenticated-orcid":false,"given":"Nima","family":"Rafezi","sequence":"first","affiliation":[{"name":"Department of Medical Engineering, University of South Florida, Tampa, FL, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sunaya A.","family":"Rahman","sequence":"additional","affiliation":[{"name":"Department of Medical Engineering, University of South Florida, Tampa, FL, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1849-2870","authenticated-orcid":false,"given":"Babak N.","family":"Safa","sequence":"additional","affiliation":[{"name":"Department of Medical Engineering, University of South Florida, Tampa, FL, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026091608150803000_2026.09.04.749502v1.1","doi-asserted-by":"publisher","DOI":"10.1016\/S0079-6123(08)01104-7"},{"key":"2026091608150803000_2026.09.04.749502v1.2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ophtha.2009.10.030"},{"key":"2026091608150803000_2026.09.04.749502v1.3","unstructured":"Association for Research in Vision and Ophthalmology (2024) ARVO Statement for the Use of Animals in Ophthalmic and Vision Research. 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