{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:19:57Z","timestamp":1792790397854,"version":"4.5.0"},"posted":{"date-parts":[[2026,9,17]]},"group-title":"Molecular Biology","reference-count":45,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T00:00:00Z","timestamp":1789603200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"National Institutes of Health","award":["R01AI128201"],"award-info":[{"award-number":["R01AI128201"]}]},{"name":"U.S. National Science Foundation","award":["2525447"],"award-info":[{"award-number":["2525447"]}],"id":[{"id":"https:\/\/ror.org\/021nxhr62","id-type":"ROR","asserted-by":"crossref"}]},{"name":"United States Department of Agriculture","award":["7011141"],"award-info":[{"award-number":["7011141"]}],"id":[{"id":"https:\/\/ror.org\/01na82s61","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Penn State University","id":[{"id":"https:\/\/ror.org\/04p491231","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Dorothy Foehr Huck and J. Lloyd Huck Endowment","award":["No number"],"award-info":[{"award-number":["No number"]}],"id":[{"id":"https:\/\/ror.org\/0229evh14","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,9,17]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>In the last decade, there has been an explosion in the development of in vivo germline genetic editing technologies in arthropods, first pioneered by the Receptor-Mediated Ovary Transduction of Cargo (ReMOT Control) method. While ReMOT Control and related technologies (DIPA-CRISPR and SYNCAS) have shown great utility for gene knock-out in many different species, consistent gene insertion has been elusive, limiting widespread adoption. We developed a novel system based on the baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV) to deliver heritable CRISPR knock-out and knock-in constructs to the arthropod germline by oral feeding. We call this method \u201cGermline Engineering by Viral Transduction\u201d (\u201cGEValT\u201c). With GEValT, newly-eclosed female and male adult arthropods are fed on virus in sugar (and\/or blood) and allowed to breed, resulting in easy generation of genetically modified offspring. GeValT is easy to use and has the potential to revolutionize gene editing methodology for researchers working across diverse arthropod species.<\/jats:p>","DOI":"10.64898\/2026.09.14.751565","type":"posted-content","created":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T11:40:20Z","timestamp":1789645220000},"source":"Crossref","is-referenced-by-count":0,"title":["Infectious transgenesis in arthropods"],"prefix":"10.64898","author":[{"given":"Najmeh","family":"Nejat","sequence":"first","affiliation":[{"name":"Department of Entomology, The Pennsylvania State University, University Park, PA, USA"},{"name":"The Center for Infectious Disease Dynamics, The Pennsylvania State University, University Park, PA, USA"},{"name":"The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Travis","family":"van Warmerdam","sequence":"additional","affiliation":[{"name":"Department of Entomology, The Pennsylvania State University, University Park, PA, USA"},{"name":"The Center for Infectious Disease Dynamics, The Pennsylvania State University, University Park, PA, USA"},{"name":"The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4050-8429","authenticated-orcid":false,"given":"Jason L.","family":"Rasgon","sequence":"additional","affiliation":[{"name":"Department of Entomology, The Pennsylvania State University, University Park, PA, USA"},{"name":"The Center for Infectious Disease Dynamics, The Pennsylvania State University, University Park, PA, USA"},{"name":"The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA, USA"},{"name":"Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA, USA"},{"name":"One Health Microbiome Center, The Pennsylvania State University, University Park, PA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026092110001064000_2026.09.14.751565v1.1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-05425-9"},{"key":"2026092110001064000_2026.09.14.751565v1.2","doi-asserted-by":"publisher","DOI":"10.1534\/g3.120.401133"},{"key":"2026092110001064000_2026.09.14.751565v1.3","doi-asserted-by":"publisher","DOI":"10.1093\/jme\/tjab016"},{"key":"2026092110001064000_2026.09.14.751565v1.4","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1089\/crispr.2019.0067","article-title":"CRISPR-Cas9-Based Genome Editing in the Silverleaf Whitefly (Bemisia tabaci)","volume":"3","year":"2020","journal-title":"CRISPR J"},{"key":"2026092110001064000_2026.09.14.751565v1.5","first-page":"103781","article-title":"Cas9-mediated gene editing in the black-legged tick","volume":"25","year":"2022","journal-title":"Ixodes scapularis, by embryo injection and ReMOT Control. iScience"},{"key":"2026092110001064000_2026.09.14.751565v1.6","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1089\/crispr.2023.0076","article-title":"Gene Editing in the Chagas Disease Vector Rhodnius prolixus by Cas9-Mediated ReMOT Control","volume":"7","year":"2024","journal-title":"CRISPR J"},{"key":"2026092110001064000_2026.09.14.751565v1.7","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2020.05.214"},{"key":"2026092110001064000_2026.09.14.751565v1.8","first-page":"1","article-title":"Delivery methods for CRISPR\/Cas9 gene editing in crustaceans","volume":"2","year":"2020","journal-title":"Mar. 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