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Despite the rapid increase in relevance and translational potential, there remains a lack of tools for studying glycosylation\n                  <jats:italic>in vivo<\/jats:italic>\n                  . Here, we present FlyMOE, a chemical-genetic platform for targeted glycoprotein profiling in\n                  <jats:italic>Drosophila melanogaster<\/jats:italic>\n                  . Transgenic flies are equipped with biosynthetic capabilities to install bioorthogonally-tagged monosaccharides into the cellular glycoproteome. The genetic tractability of\n                  <jats:italic>Drosophila<\/jats:italic>\n                  allows for versatile application based on developmental stages, genetic drivers and glycosyltransferase enzymes. We employ FlyMOE to create an overview of the glycoproteome of\n                  <jats:italic>Drosophila<\/jats:italic>\n                  tissues and embryos based on mass spectrometry glycoproteomics, and investigate the substrates of individual members of the PGANT glycosyltransferase family\n                  <jats:italic>in vivo<\/jats:italic>\n                  . FlyMOE will allow the detailed investigation of the glycoproteome in\n                  <jats:italic>Drosophila<\/jats:italic>\n                  and similarly genetically modifiable\n                  <jats:italic>in vivo<\/jats:italic>\n                  model systems.\n                <\/jats:p>","DOI":"10.64898\/2026.09.15.751681","type":"posted-content","created":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T14:15:21Z","timestamp":1789654521000},"source":"Crossref","is-referenced-by-count":0,"title":["An in vivo chemical genetic approach for targeted glycoproteome analysis in\n                  <i>Drosophila melanogaster<\/i>"],"prefix":"10.64898","author":[{"given":"Sophie D.","family":"Schmidt","sequence":"first","affiliation":[{"name":"Chemical Glycobiology Laboratory, The Francis Crick Institute, NW1 1AT London, United Kingdom"},{"name":"Department of Chemistry, Imperial College London, W12 0BZ, London, United Kingdom"},{"name":"Epithelial Cell Interactions Laboratory, The Francis Crick Institute, NW1 1AT London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cyrille","family":"Alexandre","sequence":"additional","affiliation":[{"name":"Epithelial Cell Interactions Laboratory, The Francis Crick Institute, NW1 1AT London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucia","family":"Di Vagno","sequence":"additional","affiliation":[{"name":"Chemical Glycobiology Laboratory, The Francis Crick Institute, NW1 1AT London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liping","family":"Zhang","sequence":"additional","affiliation":[{"name":"Developmental Glycobiology Section, NIDCR, National Institutes of Health, 30 Convent Drive, Bethesda, Maryland 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helen","family":"Flynn","sequence":"additional","affiliation":[{"name":"Proteomics Science Technology Platform, The Francis Crick Institute, NW1 1AT London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joachim","family":"Kurth","sequence":"additional","affiliation":[{"name":"Fly Facility, The Francis Crick Institute, NW1 1AT London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steve","family":"Murray","sequence":"additional","affiliation":[{"name":"Fly Facility, The Francis Crick Institute, NW1 1AT London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sasha Ruiz","family":"Herrera","sequence":"additional","affiliation":[{"name":"Fly Facility, The Francis Crick Institute, NW1 1AT London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannah","family":"Shaw","sequence":"additional","affiliation":[{"name":"Fly Facility, The Francis Crick Institute, NW1 1AT London, United 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USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelly G.","family":"Ten Hagen","sequence":"additional","affiliation":[{"name":"Developmental Glycobiology Section, NIDCR, National Institutes of Health, 30 Convent Drive, Bethesda, Maryland 20892"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2305-5744","authenticated-orcid":false,"given":"Jean-Paul","family":"Vincent","sequence":"additional","affiliation":[{"name":"Epithelial Cell Interactions Laboratory, The Francis Crick Institute, NW1 1AT London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5504-0147","authenticated-orcid":false,"given":"Benjamin","family":"Schumann","sequence":"additional","affiliation":[{"name":"Chemical Glycobiology Laboratory, The Francis Crick Institute, NW1 1AT London, United Kingdom"},{"name":"Department of Chemistry, Imperial College London, W12 0BZ, London, United 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