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Finding additional molecular roles for Rab GTPases depends on expanding the identification of their nucleotide-dependent interactions. Here we compare the interactome of Rab GTPases using a comprehensive large-scale yeast 2-hybrid approach powered by quantitative high-throughput sequencing, which was used to find interactions of the major mammalian Rab isoforms locked in a GDP or GTP conformation. These data revealed an expanded set of interactions specific for GTP-bound Rab proteins, with many known Rab effectors capable of binding a wider repertoire of Rab partners than previously appreciated. We also identified the RGS domain of SNX13 and SNX14 as a new Rab GTPase-binding domain that may bridge these ER-localized proteins with Rab5 and Rab11 endosomal compartments, respectively.<\/jats:p>","DOI":"10.64898\/2026.09.15.751902","type":"posted-content","created":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T20:55:12Z","timestamp":1789678512000},"source":"Crossref","is-referenced-by-count":0,"title":["Survey of nucleotide-specific Rab GTPase interactions reveals multiple Rab effectors"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0780-5795","authenticated-orcid":false,"given":"Tabitha A.","family":"Peterson","sequence":"first","affiliation":[{"name":"Department of Molecular Physiology and Biophysics, Carver College of Medicine, University of Iowa, Iowa City, Iowa, 52242"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2752-0367","authenticated-orcid":false,"given":"Christopher P.","family":"Ptak","sequence":"additional","affiliation":[{"name":"Biomolecular Nuclear Magnetic Resonance Facility Carver, College of Medicine, University of Iowa, Iowa City, Iowa, 52242"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5869-7985","authenticated-orcid":false,"given":"Mark A","family":"Stamnes","sequence":"additional","affiliation":[{"name":"Department of Molecular Physiology and Biophysics, Carver College of Medicine, University of Iowa, Iowa City, Iowa, 52242"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4032-0602","authenticated-orcid":false,"given":"Robert C.","family":"Piper","sequence":"additional","affiliation":[{"name":"Department of Molecular Physiology and Biophysics, Carver College of Medicine, University of Iowa, Iowa City, Iowa, 52242"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026101210101203000_2026.09.15.751902v2.1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22052319"},{"key":"2026101210101203000_2026.09.15.751902v2.2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-61802-1"},{"key":"2026101210101203000_2026.09.15.751902v2.3","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(99)80167-5"},{"key":"2026101210101203000_2026.09.15.751902v2.4","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.05.018"},{"key":"2026101210101203000_2026.09.15.751902v2.5","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddy101"},{"key":"2026101210101203000_2026.09.15.751902v2.6","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(00)01730-8"},{"key":"2026101210101203000_2026.09.15.751902v2.7","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00003.2012"},{"key":"2026101210101203000_2026.09.15.751902v2.8","doi-asserted-by":"publisher","DOI":"10.1002\/1873-3468.12100"},{"key":"2026101210101203000_2026.09.15.751902v2.9","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201808133"},{"key":"2026101210101203000_2026.09.15.751902v2.10","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2011124117"},{"key":"2026101210101203000_2026.09.15.751902v2.11","doi-asserted-by":"publisher","DOI":"10.1007\/bf00197809"},{"key":"2026101210101203000_2026.09.15.751902v2.12","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdg257"},{"key":"2026101210101203000_2026.09.15.751902v2.13","first-page":"211","article-title":"Ras proteins: structure, activation, and possible involvement in cancer","volume":"29","year":"1986","journal-title":"Curr. 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