{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T17:00:01Z","timestamp":1790442001893,"version":"4.1.0"},"posted":{"date-parts":[[2026,9,23]]},"group-title":"Neuroscience","reference-count":104,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"award":["88887.956451\/2024-00"],"award-info":[{"award-number":["88887.956451\/2024-00"]}],"id":[{"id":"https:\/\/ror.org\/00x0ma614","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,9,23]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Parkinson\u2019s disease (PD) is the second most prevalent neurodegenerative disease in the elderly population, characterized by a wide variety of motor and non-motor symptoms. Its complex biochemical and molecular mechanisms make it challenging to study PD directly in patients or to replicate it in vitro, underscoring the need for careful model characterization to advance translational research. The free-living nematode\n                  <jats:italic>Caenorhabditis elegans<\/jats:italic>\n                  has been widely used in neuroscience studies. Technological advancements enabled the development of transgenic strains that mimic aspects of neurodegenerative diseases, such as the OW13 strain [\n                  <jats:italic>grk-1<\/jats:italic>\n                  (ok1239) X;\n                  <jats:italic>pkIs2386<\/jats:italic>\n                  IV (\n                  <jats:italic>unc-54<\/jats:italic>\n                  p::alpha-synuclein::YFP +\n                  <jats:italic>unc-119<\/jats:italic>\n                  (+))], which expresses human \u03b1-synuclein in body wall muscle cells in a sensitized G-protein coupled receptor kinase-deficient background. This study conducted an exploratory proteomic analysis of the OW13 strain. A cross-species comparative analysis using post-mortem substantia nigra proteomics from PD individuals identified a convergent signature of 76 conserved\n                  <jats:italic>C. elegans<\/jats:italic>\n                  proteins, corresponding to 70 human orthologs, derived from the 373 DAPs. Among these conserved candidates, 68 proteins were upregulated, and 8 were downregulated. Functional enrichment linked these DAPs to the tricarboxylic acid cycle, mitochondrial function, and protein quality control, suggesting that \u03b1-synuclein aggregation triggers a broad proteostatic and bioenergetic remodeling resembling PD pathophysiology. This first proteomic and cross-species characterization of the OW13 strain strongly supports\n                  <jats:italic>C. elegans<\/jats:italic>\n                  as a robust PD model.\n                <\/jats:p>","DOI":"10.64898\/2026.09.17.752360","type":"posted-content","created":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T19:45:14Z","timestamp":1790192714000},"source":"Crossref","is-referenced-by-count":0,"title":["Label-Free Proteomics of \u03b1-Synuclein-Expressing\n                  <i>Caenorhabditis elegans<\/i>\n                  Model Uncovers Conserved Pathophysiological Pathways in Parkinson\u2019s Disease"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0524-8913","authenticated-orcid":false,"given":"Iverson Conrado","family":"Bezerra","sequence":"first","affiliation":[{"name":"Department of Computer Science, Institute of Exact Sciences (ICEx), Federal University of Minas Gerais, Belo Horizonte, Brazil"},{"name":"Keizo Asami Institute, iLIKA, Federal University of Pernambuco, Recife, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Luiza de Lima","family":"Vitorino","sequence":"additional","affiliation":[{"name":"Keizo Asami Institute, iLIKA, Federal University of Pernambuco, Recife, Brazil"},{"name":"Graduate Program in Biology Applied to Health, PPGBAS, Federal University of Pernambuco, Recife, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josivan Barbosa","family":"de Farias","sequence":"additional","affiliation":[{"name":"Keizo Asami Institute, iLIKA, Federal University of Pernambuco, Recife, Brazil"},{"name":"Graduate Program in Biology Applied to Health, PPGBAS, Federal University of Pernambuco, Recife, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Gabriela Arruda","family":"de Medeiros","sequence":"additional","affiliation":[{"name":"Keizo Asami Institute, iLIKA, Federal University of Pernambuco, Recife, Brazil"},{"name":"Undergraduate Program in Medicine, Pernambuco Health College, Recife, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto Afonso","family":"da Silva","sequence":"additional","affiliation":[{"name":"Keizo Asami Institute, iLIKA, Federal University of Pernambuco, Recife, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Luiz de Lima","family":"Filho","sequence":"additional","affiliation":[{"name":"Keizo Asami Institute, iLIKA, Federal University of Pernambuco, Recife, Brazil"},{"name":"Graduate Program in Biology Applied to Health, PPGBAS, Federal University of Pernambuco, Recife, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo Jos\u00e9 da Silva","family":"Pereira","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Federal University of S\u00e3o Paulo, UNIFESP, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3598-8050","authenticated-orcid":false,"given":"Priscila","family":"Gubert","sequence":"additional","affiliation":[{"name":"Keizo Asami Institute, iLIKA, Federal University of Pernambuco, Recife, Brazil"},{"name":"Graduate Program in Biology Applied to Health, PPGBAS, Federal University of Pernambuco, Recife, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026092609550504000_2026.09.17.752360v1.1","unstructured":"Kouli, A. ; Torsney, K. 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(Eds.), Parkinson\u2019s Disease: Pathogenesis and Clinical Aspects [Internet]; Codon Publications: Brisbane (AU), 2018, Chapter 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