{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:19:24Z","timestamp":1792790364469,"version":"4.5.0"},"posted":{"date-parts":[[2026,9,18]]},"group-title":"Molecular Biology","reference-count":31,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:00:00Z","timestamp":1789689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"National Institutes of Health","award":["R00HD109457"],"award-info":[{"award-number":["R00HD109457"]}]},{"name":"National Institutes of Health","award":["1S10OD016290-01A1"],"award-info":[{"award-number":["1S10OD016290-01A1"]}]},{"award":["P200A240099"],"award-info":[{"award-number":["P200A240099"]}],"id":[{"id":"https:\/\/ror.org\/021adze67","id-type":"ROR","asserted-by":"publisher"}]},{"name":"California Institute for Regenerative Medicine","award":["EDUC2-12720"],"award-info":[{"award-number":["EDUC2-12720"]}],"id":[{"id":"https:\/\/ror.org\/033m8b439","id-type":"ROR","asserted-by":"crossref"}]},{"name":"U.S. National Science Foundation","award":["MRI-2215705"],"award-info":[{"award-number":["MRI-2215705"]}],"id":[{"id":"https:\/\/ror.org\/021nxhr62","id-type":"ROR","asserted-by":"crossref"}]},{"name":"U.S. National Science Foundation","award":["MRI-1429826"],"award-info":[{"award-number":["MRI-1429826"]}],"id":[{"id":"https:\/\/ror.org\/021nxhr62","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,9,18]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>RNA localization to protrusions in non-neuronal cells is an emerging molecular process, distinct from the canonical mechanism characterized in neurons. Here, we describe a fractionation workflow optimized for non-neuronal cells that increases sensitivity and reproducibility of transcriptome-wide quantification of protrusion-localized RNAs. Using the optimized method, we identify six categories of protrusion-localized RNAs in non-neuronal cells from mice, including long non-coding RNAs and pseudogenes, and observe spatially distinct subsets of mitochondrially-localized RNAs. Taken together, our results reveal previously unappreciated spatial regulation of diverse transcripts and point toward broadly acting unifying principles that extend across cell types and species.<\/jats:p>","DOI":"10.64898\/2026.09.17.752430","type":"posted-content","created":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T23:15:09Z","timestamp":1789773309000},"source":"Crossref","is-referenced-by-count":0,"title":["GLOBAL ANALYSIS OF PROTRUSION-TRANCSRIPTOMES IDENTIFIES DISTINCT CATEGORIES OF LOCALIZED RNAS IN NON-NEURONAL CELLS"],"prefix":"10.64898","author":[{"given":"Briana","family":"Hojo","sequence":"first","affiliation":[{"name":"Department of Biochemistry, University of California, Riverside, 3401 Watkins Drive, Boyce Hall, Riverside, CA, 92521, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shikha","family":"Kumari","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of California, Riverside, 3401 Watkins Drive, Boyce Hall, Riverside, CA, 92521, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominic","family":"Milla","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of California, Riverside, 3401 Watkins Drive, Boyce Hall, Riverside, CA, 92521, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria","family":"Vang","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of California, Riverside, 3401 Watkins Drive, Boyce Hall, Riverside, CA, 92521, United States"},{"name":"Department of Biology, California State University, San Bernardino, 5500 University Parkway, San Bernardino, CA, 92407, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0666-2665","authenticated-orcid":false,"given":"Megan L","family":"Norris","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of California, Riverside, 3401 Watkins Drive, Boyce Hall, Riverside, CA, 92521, United States"},{"name":"Center for RNA Biology and Medicine, University of California, Riverside, Riverside, CA, 92521, United States of America"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026092303200451000_2026.09.17.752430v1.1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac763"},{"key":"2026092303200451000_2026.09.17.752430v1.2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-61940-6"},{"key":"2026092303200451000_2026.09.17.752430v1.3","doi-asserted-by":"publisher","DOI":"10.3390\/ijms242115600"},{"key":"2026092303200451000_2026.09.17.752430v1.4","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2010872117"},{"key":"2026092303200451000_2026.09.17.752430v1.5","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2020106003"},{"key":"2026092303200451000_2026.09.17.752430v1.6","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2020.10.006"},{"key":"2026092303200451000_2026.09.17.752430v1.7","doi-asserted-by":"publisher","DOI":"10.1111\/tra.12730"},{"key":"2026092303200451000_2026.09.17.752430v1.8","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.05.027"},{"key":"2026092303200451000_2026.09.17.752430v1.9","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-26365-x"},{"key":"2026092303200451000_2026.09.17.752430v1.10","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2023.06.036"},{"key":"2026092303200451000_2026.09.17.752430v1.11","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.80040"},{"key":"2026092303200451000_2026.09.17.752430v1.12","doi-asserted-by":"publisher","DOI":"10.1038\/nature10912"},{"key":"2026092303200451000_2026.09.17.752430v1.13","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2023.06.021"},{"key":"2026092303200451000_2026.09.17.752430v1.14","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8"},{"key":"2026092303200451000_2026.09.17.752430v1.15","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2025.08.002"},{"key":"2026092303200451000_2026.09.17.752430v1.16","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2015.10.005"},{"key":"2026092303200451000_2026.09.17.752430v1.17","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-60532-8"},{"key":"2026092303200451000_2026.09.17.752430v1.18","doi-asserted-by":"publisher","DOI":"10.1038\/nature06888"},{"key":"2026092303200451000_2026.09.17.752430v1.19","doi-asserted-by":"publisher","DOI":"10.15252\/embj.2020104958"},{"key":"2026092303200451000_2026.09.17.752430v1.20","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2022.103845"},{"key":"2026092303200451000_2026.09.17.752430v1.21","doi-asserted-by":"publisher","DOI":"10.1261\/rna.079426.122"},{"key":"2026092303200451000_2026.09.17.752430v1.22","doi-asserted-by":"publisher","DOI":"10.1101\/gad.350320.122"},{"key":"2026092303200451000_2026.09.17.752430v1.23","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4197"},{"key":"2026092303200451000_2026.09.17.752430v1.24","doi-asserted-by":"publisher","DOI":"10.1261\/rna.078576.120"},{"key":"2026092303200451000_2026.09.17.752430v1.25","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.05.042"},{"key":"2026092303200451000_2026.09.17.752430v1.26","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2019.11.025"},{"key":"2026092303200451000_2026.09.17.752430v1.27","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.7563.1"},{"key":"2026092303200451000_2026.09.17.752430v1.28","doi-asserted-by":"publisher","DOI":"10.1002\/wrna.1542"},{"key":"2026092303200451000_2026.09.17.752430v1.29","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-00884-y"},{"key":"2026092303200451000_2026.09.17.752430v1.30","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aaw6938"},{"key":"2026092303200451000_2026.09.17.752430v1.31","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-00690-6"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.64898\/2026.09.17.752430","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T10:20:14Z","timestamp":1790158814000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.64898\/2026.09.17.752430"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,18]]},"references-count":31,"URL":"https:\/\/doi.org\/10.64898\/2026.09.17.752430","relation":{},"subject":[],"published":{"date-parts":[[2026,9,18]]},"subtype":"preprint"}}