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Adelson Medical Research Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,10,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>Intracellular diffusion is inherently heterogeneous, yet single-particle tracking (SPT) analyses are often summarized using cell-wide average parameters that can obscure localized effects. Here, we tracked 40-nm genetically encoded multimeric (GEM) nanoparticles during stress granule (SG) formation and developed SPaCe-MC (Spatially Constrained Monte Carlo permutation test), a statistical framework that generates cytoplasm-specific null models to test whether diffusion associated with a specific cellular structure differs from that expected in the surrounding heterogeneous cytoplasm. Across three SG-inducing conditions, including oxidative stress, DDX3 inhibition, and combined treatment, bulk cytoplasmic analyses revealed distinct responses ranging from increased nanoparticle mobility to increased subdiffusive behavior. In contrast, SPaCe-MC consistently detected a local diffusion constraint in SG-associated regions relative to their treatment-matched cytoplasmic background, revealing a conserved local diffusion effect despite divergent global cytoplasmic responses. Together, our findings establish SPaCe-MC as a framework for identifying compartment-specific diffusion changes in heterogeneous cellular environments.<\/jats:p>","DOI":"10.64898\/2026.09.17.752460","type":"posted-content","created":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T21:00:17Z","timestamp":1789765217000},"source":"Crossref","is-referenced-by-count":0,"title":["Spatially Constrained Monte Carlo Permutation Test Reveals Diffusion Changes Near Stress Granules"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0840-4654","authenticated-orcid":false,"given":"Elizaveta","family":"Korunova","sequence":"first","affiliation":[{"name":"Department of Drug Discovery & Biomedical Sciences, College of Pharmacy, University of South Carolina, Columbia, South Carolina, 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