{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:19:20Z","timestamp":1792790360432,"version":"4.5.0"},"posted":{"date-parts":[[2026,9,19]]},"group-title":"Microbiology","reference-count":60,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T00:00:00Z","timestamp":1789776000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"German Research Foundation DFG","award":["Research Center 1182 on the Origin and Function of Metaorganisms (A1.2 to KD, C2.2 to PR and Z2 to RAS)"],"award-info":[{"award-number":["Research Center 1182 on the Origin and Function of Metaorganisms (A1.2 to KD, C2.2 to PR and Z2 to RAS)"]}],"id":[{"id":"https:\/\/ror.org\/018mejw64","id-type":"ROR","asserted-by":"crossref"}]},{"name":"German Research Foundation DFG","award":["individual grant 556291697 to BP"],"award-info":[{"award-number":["individual grant 556291697 to BP"]}],"id":[{"id":"https:\/\/ror.org\/018mejw64","id-type":"ROR","asserted-by":"crossref"}]},{"name":"European Crohn\u2019s and Colitis Organisation","award":["PROP-2550 to CEH"],"award-info":[{"award-number":["PROP-2550 to CEH"]}],"id":[{"id":"https:\/\/ror.org\/05beaae04","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Spanish Ministry of Science","award":["Beatriz Galindo BG24\/00107 to CEH"],"award-info":[{"award-number":["Beatriz Galindo BG24\/00107 to CEH"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,9,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Host mechanisms that regulate intestinal colonization are viewed as acting on microbes, yet microbial traits that determine susceptibility to host control remain poorly understood. Using the association between\n                  <jats:italic>Caenorhabditis elegans<\/jats:italic>\n                  and its symbiont\n                  <jats:italic>Pseudomonas lurida<\/jats:italic>\n                  MYb11, we identify the cyclic lipopeptide biosurfactant massetolide as a bacterial factor that couples microbial physiology to host-mediated clearance. An unbiased screen of &gt;11,000 bacterial mutants coupled to a host transcriptional reporter, combined with bacterial and host genetics, chemical complementation, transcriptomics, and physiological analyses, revealed that massetolide triggers host TGF-\u03b2 and serotonergic signaling to promote intestinal peristalsis, limiting colonization. Massetolide also alters bacterial surface properties and promotes swarming. Experimentally increased serotonin-dependent peristalsis selectively reduces colonization by massetolide-producing bacteria, whereas massetolide-deficient bacteria remain resistant to host-mediated expulsion. Together, our findings reveal a symbiont-derived biosurfactant that links host neuroimmune control of intestinal peristalsis with bacterial susceptibility to expulsion, coupling host and microbial physiology to regulate symbiont abundance.\n                <\/jats:p>","DOI":"10.64898\/2026.09.18.752026","type":"posted-content","created":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T03:25:15Z","timestamp":1789874715000},"source":"Crossref","is-referenced-by-count":0,"title":["A symbiont-derived biosurfactant couples bacterial surface properties to host-mediated clearance"],"prefix":"10.64898","author":[{"given":"Barbara","family":"Pees","sequence":"first","affiliation":[{"name":"Department of Evolutionary Ecology and Genetics, Zoological Institute, Christian Albrechts University, Kiel, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celia","family":"Escudero-Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Department of Paediatrics and Immunology, University of Valladolid, Valladolid, Spain"},{"name":"Institute of Clinical Molecular Biology, Christian Albrechts University and University Hospital Schleswig-Holstein, Kiel, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Avril","family":"von Hoyningen-Huene","sequence":"additional","affiliation":[{"name":"Institute for General Microbiology, Christian Albrechts University, Kiel, Germany"},{"name":"Helmholtz Institute for Functional Marine Biodiversity at the University of Oldenburg (HIFMB), Oldenburg, Germany"},{"name":"Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research, Bremerhaven, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip","family":"Rosenstiel","sequence":"additional","affiliation":[{"name":"Institute of Clinical Molecular Biology, Christian Albrechts University and University Hospital Schleswig-Holstein, Kiel, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruth A.","family":"Schmitz","sequence":"additional","affiliation":[{"name":"Institute for General Microbiology, Christian Albrechts University, Kiel, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5129-346X","authenticated-orcid":false,"given":"Katja","family":"Dierking","sequence":"additional","affiliation":[{"name":"Department of Evolutionary Ecology and Genetics, Zoological Institute, Christian Albrechts University, Kiel, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026100106101881000_2026.09.18.752026v2.1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.12.6511-6518.2000"},{"key":"2026100106101881000_2026.09.18.752026v2.2","doi-asserted-by":"crossref","unstructured":"Zhao, Y. et al. 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