{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T11:01:15Z","timestamp":1790420475001,"version":"4.1.0"},"posted":{"date-parts":[[2026,9,22]]},"group-title":"Bioengineering","reference-count":29,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T00:00:00Z","timestamp":1790035200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"award":["637308"],"award-info":[{"award-number":["637308"]}],"id":[{"id":"https:\/\/ror.org\/0472cxd90","id-type":"ROR","asserted-by":"publisher"}]},{"award":["16711"],"award-info":[{"award-number":["16711"]}],"id":[{"id":"https:\/\/ror.org\/04jsz6e67","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Dutch Province of Limburg"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,9,22]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  For regenerative medicine applications, transplanted cells are difficult to retain in a specific place to maximize the localized release of cytokines, which can positively modulate immune responses, angiogenesis and tissue regeneration. Here, we developed a hollow electrospun (ESP) scaffold with one open and one closed end to enclose a hydrogel laden with human mesenchymal stem cells (hMSCs); we termed this combination the hydrocup. hMSCs remained viable for 28 days\n                  <jats:italic>in vitro<\/jats:italic>\n                  and released functional cytokines similar to hMSCs in transplanted but non-enclosed hydrogels. The hydrocups remained intact and fixed in place for 6 weeks after subcutaneous implantation in rats, demonstrating\n                  <jats:italic>in vivo<\/jats:italic>\n                  compatibility. These results demonstrate that the hydrocup is the first, to our knowledge, cell-laden hydrogel delivery scaffold. This innovation could be developed for a wide variety of cell-based cytokine secretion or drug-laden hydrogel applications.\n                <\/jats:p>","DOI":"10.64898\/2026.09.19.752872","type":"posted-content","created":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T16:10:20Z","timestamp":1790093420000},"source":"Crossref","is-referenced-by-count":0,"title":["The hydrocup: a hollow electrospun scaffold for\n                  <i>in vivo<\/i>\n                  hydrogel-laden cell delivery"],"prefix":"10.64898","author":[{"given":"David","family":"Boaventura Gomes","sequence":"first","affiliation":[{"name":"Department of Complex Tissue Regeneration, MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Maastricht, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jip","family":"Zonderland","sequence":"additional","affiliation":[{"name":"Department of Complex Tissue Regeneration, MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Maastricht, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven","family":"Vermeulen","sequence":"additional","affiliation":[{"name":"Department of Complex Tissue Regeneration, MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Maastricht, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"Rezzola","sequence":"additional","affiliation":[{"name":"Department of Complex Tissue Regeneration, MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Maastricht, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Henriques Ferreira Louren\u00e7o","sequence":"additional","affiliation":[{"name":"Department of Complex Tissue Regeneration, MERLN Institute for Technology-Inspired Regenerative Medicine, Maastricht University, Maastricht, the 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