{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T01:57:28Z","timestamp":1790733448489,"version":"4.1.0"},"posted":{"date-parts":[[2026,9,22]]},"group-title":"Cell Biology","reference-count":62,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T00:00:00Z","timestamp":1790035200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"award":["GM127026"],"award-info":[{"award-number":["GM127026"]}],"id":[{"id":"https:\/\/ror.org\/04q48ey07","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,9,25]]},"abstract":"<jats:p>Mitogens trigger cell-cycle entry by activating E2F at the restriction point, which is followed by B-MYB\/FOXM1 activation and progression to mitosis. How mitogens control continued cycling and cell-cycle exit after the restriction point is not well-understood. By developing an E2F and B-MYB\/FOXM1 dual transcriptional biosensor system, we show that S\/G2 phase duration is set by timed mitogen-regulated B-MYB\/FOXM1 activation, while E2F activity gradually declines before mitosis. As a striking consequence, rapid B-MYB\/FOXM1 activation shortens S\/G2, delivering high mitotic E2F activity to daughter cells which keeps them cycling. Delayed B-MYB\/FOXM1 activation prolongs S\/G2, depleting mitotic E2F which drives daughters to quiescence. When S\/G2 is further prolonged, partially activated B-MYB\/FOXM1 frequently reverts, triggering mitotic bypass and polyploid quiescence. Thus, B-MYB\/FOXM1 governs a tri-directional \u201cG2 restriction point\u201d where cells commit to continued cycling through early B-MYB\/FOXM1 activation; cell-cycle exit through delayed B-MYB\/FOXM1 activation; or mitotic bypass by B-MYB\/FOXM1 inactivation.<\/jats:p>","DOI":"10.64898\/2026.09.21.753170","type":"posted-content","created":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T23:40:17Z","timestamp":1790120417000},"source":"Crossref","is-referenced-by-count":0,"title":["Parental B-MYB\/FOXM1 controls mitotic E2F to determine daughter cell fate"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8020-6206","authenticated-orcid":false,"given":"David L.","family":"Rosenthal","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Biophysics, Weill Cornell Medicine, New York, NY, 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