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When and where a protein is first translated, and how long it will remain in the cell, dictate the timing and extent of a gene\u2032s phenotypic effect. Accessing protein synthesis and turnover information in the rapidly developing embryo remains a major challenge. Here we present MEMBRYO: a method for continuous stable isotope labeling (SILAC) of newly synthesized proteins throughout the live embryo at the hour-timescales of developmental phenotypes. We extend embryo culture into the late stages of organogenesis, thereby accessing the proteome of neurogenesis in the forebrain ex utero. Variations in protein synthesis and turnover activity lead to expression patterns that diverge from the corresponding transcripts, impacting molecular pathways and key milestones of cortical neuron differentiation. The high labeling depth enabled both spatial and single-cell SILAC proteomics analysis of cortical cells, revealing distinct layers of post-transcriptional gene expression regulation in progenitors and neurons. By continuous metabolic labeling in the live embryo, MEMBRYO tracks the past, present, and future of the developmental proteome.<\/jats:p>","DOI":"10.64898\/2026.09.21.753234","type":"posted-content","created":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T23:30:18Z","timestamp":1790119818000},"source":"Crossref","is-referenced-by-count":0,"title":["Recording proteome dynamics in the late-stage embryo at hour-timescale, spatial, and single-cell resolution with MEMBRYO"],"prefix":"10.64898","author":[{"given":"Marta","family":"Couce-Iglesias","sequence":"first","affiliation":[{"name":"Max Planck Institute for Molecular Genetics;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eleonora","family":"Oliani","sequence":"additional","affiliation":[{"name":"Max Planck Institute for Molecular Genetics;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Kerridge","sequence":"additional","affiliation":[{"name":"Max Delbrueck Center for Molecular Medicine;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florian","family":"Mutschler","sequence":"additional","affiliation":[{"name":"Max Delbrueck Center for Molecular Medicine;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela S.","family":"Valdes","sequence":"additional","affiliation":[{"name":"Max Delbrueck Center for Molecular Medicine;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna Sophie","family":"Welter","sequence":"additional","affiliation":[{"name":"Max Delbrueck Center for Molecular Medicine;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beata","family":"Lukaszewska-McGreal","sequence":"additional","affiliation":[{"name":"Max Planck Institute for Molecular Genetics;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Meierhofer","sequence":"additional","affiliation":[{"name":"Max Planck Institute for Molecular Genetics;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koshi","family":"Imami","sequence":"additional","affiliation":[{"name":"RIKEN Center for Integrative Medical Sciences;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eugenio F.","family":"Fornasiero","sequence":"additional","affiliation":[{"name":"University Medical Center Goettingen"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabian","family":"Coscia","sequence":"additional","affiliation":[{"name":"Max Delbrueck Center for Molecular Medicine;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Selbach","sequence":"additional","affiliation":[{"name":"Max Delbrueck Center for Molecular Medicine;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7359-0318","authenticated-orcid":false,"given":"Matthew L.","family":"Kraushar","sequence":"additional","affiliation":[{"name":"Max Planck Institute for Molecular Genetics;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026100120551046000_2026.09.21.753234v2.1","doi-asserted-by":"crossref","unstructured":"Kraushar, M. 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