{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T14:58:13Z","timestamp":1790866693863,"version":"4.1.0"},"posted":{"date-parts":[[2026,9,28]]},"group-title":"Neuroscience","reference-count":80,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T00:00:00Z","timestamp":1790553600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,9,28]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>\n                  The preoptic area (POA) is a key regulator of body temperature, orchestrating behavioral and physiological thermoregulatory functions, including fever, torpor, and homeostatic responses to ambient temperature. Histamine regulates body temperature across animal taxa, including mammals, lower vertebrates, and invertebrates.\n                  <jats:italic>Hrh1<\/jats:italic>\n                  , which encodes the histamine H\n                  <jats:sub>1<\/jats:sub>\n                  receptor, is expressed in several neuronal populations in the POA with limited overlap with other POA thermoregulatory neurons. Acute activation of POA\n                  <jats:sup>Hrh1<\/jats:sup>\n                  neurons increased body temperature and physical activity. Acute inhibition attenuated the dark phase increase in body temperature without affecting physical activity. There was no effect of inhibition on body temperature during the light phase, fever, cold exposure, or warm exposure. Assessing behavioral thermoregulation, we found that POA\n                  <jats:sup>Hrh1<\/jats:sup>\n                  neurons stimulated nest building in both sexes, possibly contributing to cold-induced nesting behavior. Through projections to the arcuate, dorsomedial hypothalamic and tuberomammillary nuclei, POA\n                  <jats:sup>Hrh1<\/jats:sup>\n                  neurons can increase body temperature and physical activity. Overall, POA\n                  <jats:sup>Hrh1<\/jats:sup>\n                  neurons participate in several thermoregulatory functions, including daily body temperature rhythm regulation, physical activity, and nest building behavior.\n                <\/jats:p>","DOI":"10.64898\/2026.09.22.753241","type":"posted-content","created":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T18:50:25Z","timestamp":1790621425000},"source":"Crossref","is-referenced-by-count":0,"title":["Thermoregulatory roles of preoptic area histamine H\n                  <sub>1<\/sub>\n                  receptor-expressing neurons"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8877-8444","authenticated-orcid":false,"given":"Ram\u00f3n A.","family":"Pi\u00f1ol","sequence":"first","affiliation":[{"name":"Diabetes, Endocrinology, and Obesity Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, 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I.","family":"Goldschmidt","sequence":"additional","affiliation":[{"name":"Diabetes, Endocrinology, and Obesity Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Damien","family":"Kerspern","sequence":"additional","affiliation":[{"name":"Diabetes, Endocrinology, and Obesity Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soumya","family":"Kulkarni","sequence":"additional","affiliation":[{"name":"Diabetes, Endocrinology, and Obesity Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, 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