{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:20:23Z","timestamp":1792790423567,"version":"4.5.0"},"posted":{"date-parts":[[2026,9,28]]},"group-title":"Physiology","reference-count":38,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T00:00:00Z","timestamp":1790553600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"National Heart Lung and Blood Institute","award":["R01HL172813"],"award-info":[{"award-number":["R01HL172813"]}],"id":[{"id":"https:\/\/ror.org\/012pb6c26","id-type":"ROR","asserted-by":"crossref"}]},{"name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","award":["R00AR081367"],"award-info":[{"award-number":["R00AR081367"]}],"id":[{"id":"https:\/\/ror.org\/006zn3t30","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,9,28]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n                <jats:p>Light-cycle time-restricted feeding disrupts daily cardiovascular and thermoregulatory rhythms, but the molecular effects of light-cycle time-restricted feeding on the heart have been measured only at the level of total gene expression. We used Oxford Nanopore long-read RNA sequencing to resolve the full-length ventricular transcriptome from male and female mice under ad libitum feeding or light-cycle time-restricted feeding across the 24-hour cycle. Greater than 20% of cardiac transcripts represent unannotated variants of known genes absent from the current GENCODE reference annotation. Light-cycle time-restricted feeding reorganizes transcript usage across hundreds of genes, including genes encoding splicing regulators, largely without changing total gene expression. The genes affected are sex-specific, with fewer than 2% of changes shared at the gene, transcript, and transcript-usage levels. We show that transcript-level regulation is a previously underrecognized component of the cardiac response to altered feeding behavior, undetected by conventional short-read approaches.<\/jats:p>\n                <jats:sec>\n                  <jats:title>HIGHLIGHTS<\/jats:title>\n                  <jats:list list-type=\"bullet\">\n                    <jats:list-item>\n                      <jats:p>Feeding behavior remodels the cardiac transcriptome at gene and transcript levels<\/jats:p>\n                    <\/jats:list-item>\n                    <jats:list-item>\n                      <jats:p>&gt;20% of cardiac transcripts identified are absent from current reference annotations<\/jats:p>\n                    <\/jats:list-item>\n                    <jats:list-item>\n                      <jats:p>Transcript switching occurs without changes in total gene expression<\/jats:p>\n                    <\/jats:list-item>\n                    <jats:list-item>\n                      <jats:p>Feeding elicits sex-specific responses across gene, transcript, and transcript usage<\/jats:p>\n                    <\/jats:list-item>\n                  <\/jats:list>\n                <\/jats:sec>","DOI":"10.64898\/2026.09.22.753546","type":"posted-content","created":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:50:28Z","timestamp":1790643028000},"source":"Crossref","is-referenced-by-count":0,"title":["Light-cycle time-restricted feeding remodels a hidden layer of the cardiac transcriptome through sex-specific transcript switching"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0294-9282","authenticated-orcid":false,"given":"Shrishti","family":"Naidu","sequence":"first","affiliation":[{"name":"Department of Physiology, University of Kentucky, Lexington, KY, 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