{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:20:26Z","timestamp":1792790426738,"version":"4.5.0"},"posted":{"date-parts":[[2026,9,24]]},"group-title":"Plant Biology","reference-count":22,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:00:00Z","timestamp":1790208000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"National Institute of General Medical Sciences of the National Institutes of Health","award":["R35GM153298"],"award-info":[{"award-number":["R35GM153298"]}],"id":[{"id":"https:\/\/ror.org\/04q48ey07","id-type":"ROR","asserted-by":"crossref"}]},{"name":"National Research Foundation of Korea","award":["NRF-2021R1A6A3A01086358"],"award-info":[{"award-number":["NRF-2021R1A6A3A01086358"]}],"id":[{"id":"https:\/\/ror.org\/013aysd81","id-type":"ROR","asserted-by":"crossref"}]},{"name":"National Research Foundation of Korea","award":["RS202500512558"],"award-info":[{"award-number":["RS202500512558"]}],"id":[{"id":"https:\/\/ror.org\/013aysd81","id-type":"ROR","asserted-by":"crossref"}]},{"name":"National Research Foundation of Korea","award":["RS202500520276"],"award-info":[{"award-number":["RS202500520276"]}],"id":[{"id":"https:\/\/ror.org\/013aysd81","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,9,24]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Small molecules can control plant disease either by disarming the pathogen or by priming host immunity, but single compounds that do both are rare and mechanistically unexplained. Here we show that hopeaphenol (HP), a resveratrol tetramer from stilbene-producing plants, acts on histidine kinase (HK) signaling on both sides of the Arabidopsis\u2013\n                  <jats:italic>Pseudomonas<\/jats:italic>\n                  interaction. In\n                  <jats:italic>Pseudomonas syringae<\/jats:italic>\n                  pv.\n                  <jats:italic>tomato<\/jats:italic>\n                  DC3000, HP represses the type III secretion regulon and motility genes, restricts surface motility independently of effector delivery, and binds a defined subset of virulence-associated sensor kinases while reducing their autophosphorylation; comparable engagement occurs in\n                  <jats:italic>Pectobacterium atrosepticum<\/jats:italic>\n                  . Binding and inhibition depend on the tetrameric scaffold rather than the resveratrol monomer. In the host, HP binds the CHASE domains of the cytokinin receptors AHK2, AHK3 and AHK4 and elicits an immune-associated, rather than canonical cytokinin, transcriptional output. HP-dependent potentiation of PTI and ETI responses and early restriction of bacterial growth require AHK3 and AHK4. HP thus coordinates opposing outputs from divergent HK systems across kingdoms.\n                <\/jats:p>","DOI":"10.64898\/2026.09.23.753455","type":"posted-content","created":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T14:45:20Z","timestamp":1790261120000},"source":"Crossref","is-referenced-by-count":0,"title":["Hopeaphenol targets histidine kinase signaling across kingdoms to suppress bacterial virulence and potentiate plant immunity"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1455-8641","authenticated-orcid":false,"given":"Jieun","family":"Kang","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48824, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nayeon","family":"Yoo","sequence":"additional","affiliation":[{"name":"Department of Plant Biotechnology, Korea University, Seoul, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3592-6597","authenticated-orcid":false,"given":"Talia","family":"Karasov","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, University of Utah, Salt Lake City, UT 84112, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Venkatesh P","family":"Thirumlaikumar","sequence":"additional","affiliation":[{"name":"Purdue University, West Lafayette, IN 47907, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lydia","family":"Story","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48824, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eui-Hwan","family":"Chung","sequence":"additional","affiliation":[{"name":"Department of Plant Biotechnology, Korea University, Seoul, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7627-7925","authenticated-orcid":false,"given":"Aleksandra","family":"Skirycz","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48824, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"issue":"1","key":"2026092708100521000_2026.09.23.753455v1.1","doi-asserted-by":"crossref","first-page":"e1002448","DOI":"10.1371\/journal.pgen.1002448","article-title":"Two-component elements mediate interactions between cytokinin and salicylic acid in plant immunity","volume":"8","year":"2012","journal-title":"PLoS 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(2021). Pseudomonas syringae senses polyphenols via phosphorelay crosstalk to inhibit virulence. The EMBO Reports 22(12), EMBR202152805.","DOI":"10.15252\/embr.202152805"},{"key":"2026092708100521000_2026.09.23.753455v1.21","doi-asserted-by":"crossref","first-page":"3415","DOI":"10.1016\/j.csbj.2020.10.039","article-title":"Signal transduction schemes in Pseudomonas syringae","volume":"18","year":"2020","journal-title":"Computational and Structural Biotechnology Journal"},{"key":"2026092708100521000_2026.09.23.753455v1.22","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.02838-02818"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.64898\/2026.09.23.753455","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T15:10:14Z","timestamp":1790521814000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.64898\/2026.09.23.753455"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,24]]},"references-count":22,"URL":"https:\/\/doi.org\/10.64898\/2026.09.23.753455","relation":{},"subject":[],"published":{"date-parts":[[2026,9,24]]},"subtype":"preprint"}}