{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T17:53:30Z","timestamp":1791395610911,"version":"4.3.3"},"posted":{"date-parts":[[2026,9,29]]},"group-title":"Neuroscience","reference-count":29,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"award":["GM150832"],"award-info":[{"award-number":["GM150832"]}],"id":[{"id":"https:\/\/ror.org\/04q48ey07","id-type":"ROR","asserted-by":"publisher"}]},{"award":["RR220021"],"award-info":[{"award-number":["RR220021"]}],"id":[{"id":"https:\/\/ror.org\/0003xa228","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,10,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Chronic social isolation induces persistent behavioral and physiological changes across species. In humans, prolonged social isolation is associated with insomnia and increased risks of cognitive decline, cardiovascular disease, compromised immunity, and premature mortality. Yet whether these consequences are modified by the environment in which social isolation is experienced remains unclear. In particular, can access to social sensory information from conspecifics alleviate the effects of isolation and can various ambient environmental settings modify them? A related question arises during behavioral measurement because behavioral assays themselves impose a new environmental context. Whether these assays simply capture the enduring effects of prior social experience or further modify those effects remains unknown. Using social isolation-induced sleep loss in\n                  <jats:italic>Drosophila<\/jats:italic>\n                  as a model, we systematically examined how the social, spatial, and photoperiodic environments during isolation and behavioral monitoring influence the isolation phenotype. Providing isolated flies with visual, olfactory, or tactile social cues from conspecifics living behind a divider, either individually or in combination, was insufficient to alleviate sleep loss. Spatial confinement exacerbated isolation-induced sleep loss, while winter-like photoperiod in spatially confined\n                  <jats:italic>Drosophila<\/jats:italic>\n                  Activity Monitor assays revealed the clearest progressive decline in sleep during sleep monitoring. Together, these findings demonstrate that social isolation-induced sleep loss is both enduring and dynamic: it persists despite exposure to social cues yet remains sensitive to the environmental context in which isolation is experienced and behavior is measured. More broadly, our study establishes a tractable framework for investigating the environmental modulation of chronic social isolation.\n                <\/jats:p>","DOI":"10.64898\/2026.09.23.753895","type":"posted-content","created":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T17:35:19Z","timestamp":1790703319000},"source":"Crossref","is-referenced-by-count":1,"title":["Environmental modulation of social isolation-induced sleep loss in\n                  <i>Drosophila<\/i>"],"prefix":"10.64898","author":[{"given":"Brissa","family":"Castillo","sequence":"first","affiliation":[{"name":"Center for Biological Clocks Research, Department of Biology, Texas A&M University, College Station, Texas, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fiona","family":"Gugala","sequence":"additional","affiliation":[{"name":"Center for Biological Clocks Research, Department of Biology, Texas A&M University, College Station, Texas, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yangyuan","family":"Li","sequence":"additional","affiliation":[{"name":"Center for Biological Clocks Research, Department of Biology, Texas A&M University, College Station, Texas, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feda","family":"Hammood","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Hunter College, New York, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5682-5173","authenticated-orcid":false,"given":"Wanhe","family":"Li","sequence":"additional","affiliation":[{"name":"Center for Biological Clocks Research, Department of Biology, Texas A&M University, College Station, Texas, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026100710101588000_2026.09.23.753895v2.1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2011.06028.x"},{"issue":"3","key":"2026100710101588000_2026.09.23.753895v2.2","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1001\/jama.2023.11958","article-title":"Meta-Analysis: Social Isolation, Loneliness Tied to Higher Mortality","volume":"330","year":"2023","journal-title":"JAMA"},{"key":"2026100710101588000_2026.09.23.753895v2.3","doi-asserted-by":"publisher","DOI":"10.1002\/wps.21224"},{"key":"2026100710101588000_2026.09.23.753895v2.4","unstructured":"Office of the Surgeon, G., Publications and Reports of the Surgeon General, in Our Epidemic of Loneliness and Isolation: The U.S. Surgeon General\u2019s Advisory on the Healing Effects of Social Connection and Community. 2023, US Department of Health and Human Services: Washington (DC)."},{"key":"2026100710101588000_2026.09.23.753895v2.5","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1219686110"},{"key":"2026100710101588000_2026.09.23.753895v2.6","doi-asserted-by":"crossref","unstructured":"Stokes, A.C. , et al., Loneliness, social isolation, and all-cause mortality in the United States. 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