{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:19:24Z","timestamp":1792790364963,"version":"4.5.0"},"posted":{"date-parts":[[2026,9,29]]},"group-title":"Neuroscience","reference-count":128,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"National Institutes of Health, National Institute on Aging","award":["AG078859"],"award-info":[{"award-number":["AG078859"]}],"id":[{"id":"https:\/\/ror.org\/049v75w11","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,9,29]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>\n                  Apigenin is an abundant, bioactive phytochemical with reported therapeutic potential and neuroprotective effects in a variety of age-related disease models. However, the extent to which apigenin may promote healthspan and protect against neurodegeneration-associated proteotoxicity has not been thoroughly investigated, and the biological processes\/mechanisms that underlie its protective effects remain unclear. To determine if apigenin increases healthspan, we performed lifespan assays in conjunction with measures of aging-relevant physiological function in wild-type\n                  <jats:italic>C. elegans<\/jats:italic>\n                  . We found that chronic exposure to apigenin extended median lifespan and improved multiple readouts of healthspan, including pharyngeal pumping, locomotor activity, and age-related macromolecular damage accumulation. Additional analyses of transgenic reporter strains and transcriptomic profiling (RNA-seq) indicated that apigenin primarily modulated redox-sensitive stress signaling and pathways associated with cellular homeostasis in aging animals. Finally, in transgenic\n                  <jats:italic>C. elegans<\/jats:italic>\n                  strains expressing the pathological Alzheimer\u2019s disease-associated proteins amyloid-beta or tau, we found that apigenin had similar beneficial effects on lifespan, healthspan, and proteotoxicity-associated functional impairments. Together, our results support apigenin as a potential healthspan-promoting intervention that acts in part through reductions in oxidative stress-associated signaling, with beneficial effects that extend to models of Alzheimer\u2019s disease-associated proteotoxicity.\n                <\/jats:p>","DOI":"10.64898\/2026.09.23.753917","type":"posted-content","created":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T18:15:22Z","timestamp":1790705722000},"source":"Crossref","is-referenced-by-count":0,"title":["Apigenin Improves Healthspan and Protects Against Alzheimer\u2019s Disease-Associated Proteotoxicity in\n                  <i>C. elegans<\/i>"],"prefix":"10.64898","author":[{"given":"Randy A.","family":"Grant","sequence":"first","affiliation":[{"name":"Department of Health & Exercise Science, Colorado State University, Fort Collins, CO 80523, USA"},{"name":"Center for Healthy Aging, Colorado State University, Fort Collins, CO 80523, USA"},{"name":"Molecular, Cellular, & Integrative Neuroscience, Colorado State University, Fort Collins, CO 80523, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren H. E.","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Health & Exercise Science, Colorado State University, Fort Collins, CO 80523, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Devin","family":"Wahl","sequence":"additional","affiliation":[{"name":"Department of Health & Exercise Science, Colorado State University, Fort Collins, CO 80523, USA"},{"name":"Center for Healthy Aging, Colorado State University, Fort Collins, CO 80523, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas J.","family":"LaRocca","sequence":"additional","affiliation":[{"name":"Department of Health & Exercise Science, Colorado State University, Fort Collins, CO 80523, USA"},{"name":"Center for Healthy Aging, Colorado State University, Fort Collins, CO 80523, USA"},{"name":"Molecular, Cellular, & Integrative Neuroscience, Colorado State University, Fort Collins, CO 80523, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026100503200817000_2026.09.23.753917v1.1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2022.11.001"},{"key":"2026100503200817000_2026.09.23.753917v1.2","doi-asserted-by":"publisher","DOI":"10.1038\/s41582-019-0244-7"},{"key":"2026100503200817000_2026.09.23.753917v1.3","doi-asserted-by":"publisher","DOI":"10.1038\/s41416-019-0721-1"},{"key":"2026100503200817000_2026.09.23.753917v1.4","doi-asserted-by":"publisher","DOI":"10.1113\/JP270538"},{"key":"2026100503200817000_2026.09.23.753917v1.5","doi-asserted-by":"publisher","DOI":"10.1038\/s41593-018-0101-9"},{"key":"2026100503200817000_2026.09.23.753917v1.6","doi-asserted-by":"publisher","DOI":"10.3389\/fpubh.2017.00335"},{"key":"2026100503200817000_2026.09.23.753917v1.7","doi-asserted-by":"publisher","DOI":"10.1111\/acel.12861"},{"key":"2026100503200817000_2026.09.23.753917v1.8","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-023-02505-2"},{"key":"2026100503200817000_2026.09.23.753917v1.9","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-021-06512-9"},{"key":"2026100503200817000_2026.09.23.753917v1.10","doi-asserted-by":"publisher","DOI":"10.1007\/s12668-022-00998-8"},{"key":"2026100503200817000_2026.09.23.753917v1.11","doi-asserted-by":"publisher","DOI":"10.1016\/j.mad.2023.111889"},{"key":"2026100503200817000_2026.09.23.753917v1.12","doi-asserted-by":"publisher","DOI":"10.3390\/nu14214637"},{"key":"2026100503200817000_2026.09.23.753917v1.13","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25105569"},{"key":"2026100503200817000_2026.09.23.753917v1.14","doi-asserted-by":"publisher","DOI":"10.3389\/fnut.2024.1359176"},{"key":"2026100503200817000_2026.09.23.753917v1.15","doi-asserted-by":"publisher","DOI":"10.3390\/molecules18089949"},{"key":"2026100503200817000_2026.09.23.753917v1.16","doi-asserted-by":"publisher","DOI":"10.3389\/fnagi.2020.00119"},{"key":"2026100503200817000_2026.09.23.753917v1.17","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2022.110120"},{"key":"2026100503200817000_2026.09.23.753917v1.18","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2018.05.011"},{"key":"2026100503200817000_2026.09.23.753917v1.19","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2022.12.032"},{"key":"2026100503200817000_2026.09.23.753917v1.20","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-021-01382-x"},{"key":"2026100503200817000_2026.09.23.753917v1.21","doi-asserted-by":"publisher","DOI":"10.1038\/nrd1330"},{"key":"2026100503200817000_2026.09.23.753917v1.22","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3232"},{"key":"2026100503200817000_2026.09.23.753917v1.23","doi-asserted-by":"publisher","DOI":"10.1038\/s41582-020-0333-7"},{"key":"2026100503200817000_2026.09.23.753917v1.24","doi-asserted-by":"publisher","DOI":"10.1080\/10942912.2016.1207188"},{"key":"2026100503200817000_2026.09.23.753917v1.25","doi-asserted-by":"publisher","DOI":"10.1186\/1476-4598-5-76"},{"key":"2026100503200817000_2026.09.23.753917v1.26","doi-asserted-by":"publisher","DOI":"10.1111\/jfbc.12376"},{"key":"2026100503200817000_2026.09.23.753917v1.27","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.115.176099"},{"key":"2026100503200817000_2026.09.23.753917v1.28","doi-asserted-by":"publisher","DOI":"10.1159\/000090736"},{"key":"2026100503200817000_2026.09.23.753917v1.29","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2021.681477"},{"key":"2026100503200817000_2026.09.23.753917v1.30","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0056877"},{"key":"2026100503200817000_2026.09.23.753917v1.31","doi-asserted-by":"publisher","DOI":"10.1895\/wormbook.1.101.1"},{"key":"2026100503200817000_2026.09.23.753917v1.32","doi-asserted-by":"publisher","DOI":"10.1111\/acel.13798"},{"key":"2026100503200817000_2026.09.23.753917v1.33","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-73002-6"},{"key":"2026100503200817000_2026.09.23.753917v1.34","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0400848101"},{"key":"2026100503200817000_2026.09.23.753917v1.35","unstructured":"WormLab. Williston, VT USA: MBF Biosciences LLC; 2023."},{"key":"2026100503200817000_2026.09.23.753917v1.36","doi-asserted-by":"publisher","DOI":"10.13140\/RG.2.1.1307.8008"},{"key":"2026100503200817000_2026.09.23.753917v1.37","doi-asserted-by":"publisher","DOI":"10.1111\/acel.13167"},{"key":"2026100503200817000_2026.09.23.753917v1.38","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/glaa302"},{"key":"2026100503200817000_2026.09.23.753917v1.39","doi-asserted-by":"publisher","DOI":"10.1007\/s11357-024-01126-y"},{"key":"2026100503200817000_2026.09.23.753917v1.40","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2023.1263369"},{"key":"2026100503200817000_2026.09.23.753917v1.41","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty560"},{"key":"2026100503200817000_2026.09.23.753917v1.42","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts635"},{"key":"2026100503200817000_2026.09.23.753917v1.43","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8"},{"key":"2026100503200817000_2026.09.23.753917v1.44","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz369"},{"key":"2026100503200817000_2026.09.23.753917v1.45","doi-asserted-by":"publisher","DOI":"10.3791\/2252"},{"key":"2026100503200817000_2026.09.23.753917v1.46","doi-asserted-by":"publisher","DOI":"10.1007\/s11357-021-00436-9"},{"key":"2026100503200817000_2026.09.23.753917v1.47","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20061305"},{"key":"2026100503200817000_2026.09.23.753917v1.48","doi-asserted-by":"publisher","DOI":"10.1039\/C8LC00103K"},{"key":"2026100503200817000_2026.09.23.753917v1.49","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2025.103732"},{"key":"2026100503200817000_2026.09.23.753917v1.50","doi-asserted-by":"publisher","DOI":"10.1038\/s41514-021-00061-y"},{"key":"2026100503200817000_2026.09.23.753917v1.51","doi-asserted-by":"publisher","DOI":"10.1016\/j.xpro.2021.100309"},{"key":"2026100503200817000_2026.09.23.753917v1.52","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2010.05.502"},{"key":"2026100503200817000_2026.09.23.753917v1.53","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1009185"},{"key":"2026100503200817000_2026.09.23.753917v1.54","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-38210-w"},{"key":"2026100503200817000_2026.09.23.753917v1.55","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines12061353"},{"key":"2026100503200817000_2026.09.23.753917v1.56","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.107.072587"},{"key":"2026100503200817000_2026.09.23.753917v1.57","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.0020183"},{"key":"2026100503200817000_2026.09.23.753917v1.58","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1324805"},{"key":"2026100503200817000_2026.09.23.753917v1.59","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4439(00)00040-5"},{"key":"2026100503200817000_2026.09.23.753917v1.60","doi-asserted-by":"publisher","DOI":"10.3892\/br.2016.630"},{"key":"2026100503200817000_2026.09.23.753917v1.61","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2017.10.014"},{"key":"2026100503200817000_2026.09.23.753917v1.62","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2023.175584"},{"key":"2026100503200817000_2026.09.23.753917v1.63","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2014.1666"},{"key":"2026100503200817000_2026.09.23.753917v1.64","doi-asserted-by":"publisher","DOI":"10.1007\/s12565-019-00486-2"},{"key":"2026100503200817000_2026.09.23.753917v1.65","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/151979"},{"key":"2026100503200817000_2026.09.23.753917v1.66","doi-asserted-by":"publisher","DOI":"10.1007\/s11095-010-0089-7"},{"key":"2026100503200817000_2026.09.23.753917v1.67","doi-asserted-by":"publisher","DOI":"10.1111\/1440-1681.13725"},{"key":"2026100503200817000_2026.09.23.753917v1.68","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24021498"},{"key":"2026100503200817000_2026.09.23.753917v1.69","doi-asserted-by":"publisher","DOI":"10.15430\/JCP.2016.21.4.216"},{"key":"2026100503200817000_2026.09.23.753917v1.70","doi-asserted-by":"publisher","DOI":"10.2174\/1871520615666150304120643"},{"key":"2026100503200817000_2026.09.23.753917v1.71","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27186051"},{"key":"2026100503200817000_2026.09.23.753917v1.72","doi-asserted-by":"publisher","DOI":"10.1007\/s12012-021-09662-1"},{"key":"2026100503200817000_2026.09.23.753917v1.73","doi-asserted-by":"publisher","DOI":"10.3390\/nu15020385"},{"key":"2026100503200817000_2026.09.23.753917v1.74","doi-asserted-by":"publisher","DOI":"10.1211\/jpp.59.11.0012"},{"key":"2026100503200817000_2026.09.23.753917v1.75","doi-asserted-by":"publisher","DOI":"10.2174\/1389557523666230811092423"},{"key":"2026100503200817000_2026.09.23.753917v1.76","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2020.104870"},{"key":"2026100503200817000_2026.09.23.753917v1.77","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.26403"},{"key":"2026100503200817000_2026.09.23.753917v1.78","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0110429"},{"key":"2026100503200817000_2026.09.23.753917v1.79","doi-asserted-by":"publisher","DOI":"10.1016\/j.bionut.2014.07.006"},{"key":"2026100503200817000_2026.09.23.753917v1.80","doi-asserted-by":"publisher","DOI":"10.1177\/09603271211009964"},{"key":"2026100503200817000_2026.09.23.753917v1.81","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2017.10.008"},{"key":"2026100503200817000_2026.09.23.753917v1.82","doi-asserted-by":"publisher","DOI":"10.1038\/srep31450"},{"key":"2026100503200817000_2026.09.23.753917v1.83","doi-asserted-by":"publisher","DOI":"10.1186\/s10020-024-00977-7"},{"key":"2026100503200817000_2026.09.23.753917v1.84","doi-asserted-by":"publisher","DOI":"10.1080\/00207454.2022.2089136"},{"key":"2026100503200817000_2026.09.23.753917v1.85","doi-asserted-by":"publisher","DOI":"10.1007\/s12672-022-00505-1"},{"key":"2026100503200817000_2026.09.23.753917v1.86","doi-asserted-by":"publisher","DOI":"10.1111\/jfbc.13950"},{"key":"2026100503200817000_2026.09.23.753917v1.87","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.6647"},{"key":"2026100503200817000_2026.09.23.753917v1.88","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2016.5495"},{"key":"2026100503200817000_2026.09.23.753917v1.89","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2004.08.023"},{"key":"2026100503200817000_2026.09.23.753917v1.90","doi-asserted-by":"publisher","DOI":"10.1007\/s10787-024-01538-4"},{"key":"2026100503200817000_2026.09.23.753917v1.91","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25095041"},{"key":"2026100503200817000_2026.09.23.753917v1.92","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jnatprod.2c00947"},{"key":"2026100503200817000_2026.09.23.753917v1.93","doi-asserted-by":"publisher","DOI":"10.1080\/10942912.2023.2236329"},{"key":"2026100503200817000_2026.09.23.753917v1.94","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2023.e22290"},{"key":"2026100503200817000_2026.09.23.753917v1.95","doi-asserted-by":"publisher","DOI":"10.1007\/s11030-023-10607-9"},{"key":"2026100503200817000_2026.09.23.753917v1.96","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2023.03.264"},{"key":"2026100503200817000_2026.09.23.753917v1.97","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2020.103957"},{"key":"2026100503200817000_2026.09.23.753917v1.98","doi-asserted-by":"publisher","DOI":"10.1016\/j.nutres.2022.02.003"},{"key":"2026100503200817000_2026.09.23.753917v1.99","doi-asserted-by":"publisher","DOI":"10.21608\/ejhm.2025.428202.1850"},{"key":"2026100503200817000_2026.09.23.753917v1.100","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/glaa214"},{"key":"2026100503200817000_2026.09.23.753917v1.101","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.20962"},{"key":"2026100503200817000_2026.09.23.753917v1.102","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2008.21"},{"key":"2026100503200817000_2026.09.23.753917v1.103","doi-asserted-by":"publisher","DOI":"10.1111\/acel.12896"},{"key":"2026100503200817000_2026.09.23.753917v1.104","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1107803"},{"key":"2026100503200817000_2026.09.23.753917v1.105","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/1826303"},{"key":"2026100503200817000_2026.09.23.753917v1.106","doi-asserted-by":"publisher","DOI":"10.18632\/aging.101654"},{"key":"2026100503200817000_2026.09.23.753917v1.107","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.02070-06"},{"key":"2026100503200817000_2026.09.23.753917v1.108","doi-asserted-by":"publisher","DOI":"10.3390\/cells12182223"},{"key":"2026100503200817000_2026.09.23.753917v1.109","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2024.109484"},{"key":"2026100503200817000_2026.09.23.753917v1.110","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.258456"},{"key":"2026100503200817000_2026.09.23.753917v1.111","doi-asserted-by":"publisher","DOI":"10.1007\/s10522-020-09856-0"},{"key":"2026100503200817000_2026.09.23.753917v1.112","doi-asserted-by":"publisher","DOI":"10.2174\/0118746098292667240914024812"},{"key":"2026100503200817000_2026.09.23.753917v1.113","doi-asserted-by":"publisher","DOI":"10.1177\/1947601912475079"},{"key":"2026100503200817000_2026.09.23.753917v1.114","doi-asserted-by":"publisher","DOI":"10.1016\/j.mad.2020.111215"},{"key":"2026100503200817000_2026.09.23.753917v1.115","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.16.2.237"},{"key":"2026100503200817000_2026.09.23.753917v1.116","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.04.036"},{"key":"2026100503200817000_2026.09.23.753917v1.117","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/7543973"},{"key":"2026100503200817000_2026.09.23.753917v1.118","doi-asserted-by":"publisher","DOI":"10.1007\/s10522-009-9239-x"},{"key":"2026100503200817000_2026.09.23.753917v1.119","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/546915"},{"key":"2026100503200817000_2026.09.23.753917v1.120","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2015.02.002"},{"key":"2026100503200817000_2026.09.23.753917v1.121","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.19.8905"},{"key":"2026100503200817000_2026.09.23.753917v1.122","doi-asserted-by":"publisher","DOI":"10.1111\/j.1474-9726.2009.00473.x"},{"key":"2026100503200817000_2026.09.23.753917v1.123","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-35899-1"},{"key":"2026100503200817000_2026.09.23.753917v1.124","doi-asserted-by":"publisher","DOI":"10.1038\/s41583-019-0132-6"},{"key":"2026100503200817000_2026.09.23.753917v1.125","doi-asserted-by":"publisher","DOI":"10.2174\/1871527322666230406082625"},{"key":"2026100503200817000_2026.09.23.753917v1.126","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2014.00279"},{"key":"2026100503200817000_2026.09.23.753917v1.127","doi-asserted-by":"publisher","DOI":"10.1186\/1750-1326-7-57"},{"key":"2026100503200817000_2026.09.23.753917v1.128","doi-asserted-by":"publisher","DOI":"10.3389\/fnagi.2021.758895"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.64898\/2026.09.23.753917","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T10:20:15Z","timestamp":1791195615000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.64898\/2026.09.23.753917"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,29]]},"references-count":128,"URL":"https:\/\/doi.org\/10.64898\/2026.09.23.753917","relation":{},"subject":[],"published":{"date-parts":[[2026,9,29]]},"subtype":"preprint"}}