{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:19:19Z","timestamp":1792790359065,"version":"4.5.0"},"posted":{"date-parts":[[2026,9,29]]},"group-title":"Bioinformatics","reference-count":36,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"National Institute of Diabetes and Digestive and Kidney Diseases","award":["R01-DK132888"],"award-info":[{"award-number":["R01-DK132888"]}],"id":[{"id":"https:\/\/ror.org\/00adh9b73","id-type":"ROR","asserted-by":"crossref"}]},{"name":"National Institute of Diabetes and Digestive and Kidney Diseases","award":["R01-DK136297"],"award-info":[{"award-number":["R01-DK136297"]}],"id":[{"id":"https:\/\/ror.org\/00adh9b73","id-type":"ROR","asserted-by":"crossref"}]},{"name":"National Institute of Diabetes and Digestive and Kidney Diseases","award":["R01-DK142422"],"award-info":[{"award-number":["R01-DK142422"]}],"id":[{"id":"https:\/\/ror.org\/00adh9b73","id-type":"ROR","asserted-by":"crossref"}]},{"name":"National Institute of Diabetes and Digestive and Kidney Diseases","award":["R01-DK144860"],"award-info":[{"award-number":["R01-DK144860"]}],"id":[{"id":"https:\/\/ror.org\/00adh9b73","id-type":"ROR","asserted-by":"crossref"}]},{"award":["IK6-BX006823"],"award-info":[{"award-number":["IK6-BX006823"]}],"id":[{"id":"https:\/\/ror.org\/05rsv9s98","id-type":"ROR","asserted-by":"publisher"}]},{"award":["I01-BX006080"],"award-info":[{"award-number":["I01-BX006080"]}],"id":[{"id":"https:\/\/ror.org\/05rsv9s98","id-type":"ROR","asserted-by":"publisher"}]},{"name":"KidneyCure","award":["Transition to Independence Grant"],"award-info":[{"award-number":["Transition to Independence Grant"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,9,29]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:sec>\n                  <jats:title>Objective<\/jats:title>\n                  <jats:p>The maturation of an autologous arteriovenous fistula (AVF) depends on the coordinated hemodynamic adaptation of the inflow artery and the outflow vein. While venous remodeling has been extensively studied, the mechanisms of arterial adaptation are poorly understood. We addressed this knowledge gap with the first temporal single-cell atlas of the porcine AVF model.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Six Yorkshire pigs underwent bilateral femoro-femoral AVF creation for tissue harvest at 2 and 21 days postoperatively or sham operation to collect native vessels after 2 days. Arteries and veins were analyzed by droplet-based single-cell RNA sequencing, histology, and immunohistochemistry.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>\n                    All fistulas were patent, with flows reaching 1185.0 \u00b1 170.6 mL\/min despite mild to moderate neointima formation in the veins. Arteries and veins demonstrated &gt;60% endothelial denudation and increased neovascularization. Arterial remodeling was characterized by a progressive increase in venous-like transcriptional features. Arteries presented a venous-like endothelial cell (EC) composition at 21 days postop and a temporal increase in venous-associated phenotypes of myofibroblasts and fibroblasts. In both vessels, the latter populations were the main bearers of mechanosensitive signatures at 2 days. Despite the temporal convergence in phenotypes, arteries and veins retained vessel-selective characteristics. Arterial ECs expressed high levels of nitric oxide synthase, whereas venous ECs were largely inflammatory. Arterial myofibroblasts and fibroblasts had higher expression of Notch signaling transducers (\n                    <jats:italic>HEY2, HEYL<\/jats:italic>\n                    ) and cellular communication network factors (\n                    <jats:italic>CCN3, CCN5<\/jats:italic>\n                    ), while Wnt signaling regulators (\n                    <jats:italic>DKK2, SFRP4<\/jats:italic>\n                    ) and complement genes (\n                    <jats:italic>C3, C7, CFD<\/jats:italic>\n                    ) were upregulated in veins. Anti-inflammatory macrophages increased in both vessels during remodeling, likely contributing to the maturation of the fistulas.\n                  <\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>These analyses challenge the traditional paradigm of exclusive vein arterialization and support a complementary process of arterial venification after anastomosis. Recognition of this underappreciated phenomenon may reshape our understanding of AVF biology and guide the discovery of both vascular-wide and vessel-selective anti-stenotic therapies.<\/jats:p>\n                <\/jats:sec>","DOI":"10.64898\/2026.09.23.753931","type":"posted-content","created":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T20:10:21Z","timestamp":1790712621000},"source":"Crossref","is-referenced-by-count":0,"title":["The Venification of the Artery: Insights from Single-Cell Transcriptomics of a Porcine Arteriovenous Fistula Model"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-3177-0713","authenticated-orcid":false,"given":"Matthew S.","family":"Sussman","sequence":"first","affiliation":[{"name":"DeWitt Daughtry Family Department of Surgery, Leonard M. Miller School of Medicine, University of Miami, Miami, FL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8314-7777","authenticated-orcid":false,"given":"Marwan","family":"Tabbara","sequence":"additional","affiliation":[{"name":"DeWitt Daughtry Family Department of Surgery, Leonard M. Miller School of Medicine, University of Miami, Miami, FL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-5566-369X","authenticated-orcid":false,"given":"Xochilt","family":"Labissiere","sequence":"additional","affiliation":[{"name":"DeWitt Daughtry Family Department of Surgery, Leonard M. Miller School of Medicine, University of Miami, Miami, FL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1987-5555","authenticated-orcid":false,"given":"Juan S.","family":"Lopez-McCormick","sequence":"additional","affiliation":[{"name":"DeWitt Daughtry Family Department of Surgery, Leonard M. Miller School of Medicine, University of Miami, Miami, FL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Santiago J.","family":"Guzman","sequence":"additional","affiliation":[{"name":"DeWitt Daughtry Family Department of Surgery, Leonard M. Miller School of Medicine, University of Miami, Miami, FL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina","family":"Kosanovic","sequence":"additional","affiliation":[{"name":"John P. Hussman Institute for Human Genomics, University of Miami, Miami, FL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9370-276X","authenticated-orcid":false,"given":"Roberto I.","family":"Vazquez-Padron","sequence":"additional","affiliation":[{"name":"DeWitt Daughtry Family Department of Surgery, Leonard M. Miller School of Medicine, University of Miami, Miami, FL"},{"name":"Bruce W. Carter Veterans Affairs Medical Center, Miami, FL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3009-8761","authenticated-orcid":false,"given":"Laisel","family":"Martinez","sequence":"additional","affiliation":[{"name":"DeWitt Daughtry Family Department of Surgery, Leonard M. 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