{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T18:58:13Z","timestamp":1790794693094,"version":"4.1.0"},"posted":{"date-parts":[[2026,9,28]]},"group-title":"Cell Biology","reference-count":64,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T00:00:00Z","timestamp":1790553600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"funder":[{"id":[{"id":"https:\/\/ror.org\/00knt4f32","id-type":"ROR","asserted-by":"publisher"}]},{"id":[{"id":"https:\/\/ror.org\/04537p149","id-type":"ROR","asserted-by":"publisher"}]},{"name":"NSF-GRFP"},{"name":"NSF","award":["2322801"],"award-info":[{"award-number":["2322801"]}]},{"award":["DP2GM149752"],"award-info":[{"award-number":["DP2GM149752"]}],"id":[{"id":"https:\/\/ror.org\/001d55x84","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Dr. Miriam and Sheldon G. Adelson Medical Research Foundation"},{"name":"NIH-NIGMS","award":["R35GM149319"],"award-info":[{"award-number":["R35GM149319"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,9,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>Nucleosomes regulate chromatin folding, accessibility, and factor recruitment. Current models primarily attribute these functions to histone tail modifications, while the core is largely viewed as a structural scaffold. Yet subtle changes within the nucleosome core can produce profound functional consequences, and the mechanisms underlying these effects remain unclear. Here, we describe nucleosome core allostery as a fundamental principle of chromatin regulation that amplifies the impact of minimal nucleosome variations. Leveraging natural differences between H2A.Z variants, we show that the nucleosome core encodes distinct conformational dynamics that propagate allosterically, thereby controlling nucleosome accessibility and recognition by chromatin factors. As a result, a single buried amino acid substitution alone is sufficient to reprogram nucleosome dynamics and bias cell identity. Our findings establish the nucleosome core as an allosteric regulatory module and provide a generalizable framework for how subtle variation within nucleosomes is amplified into diverse biological outcomes in development and disease.<\/jats:p>","DOI":"10.64898\/2026.09.24.753912","type":"posted-content","created":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T09:50:15Z","timestamp":1790589015000},"source":"Crossref","is-referenced-by-count":0,"title":["Nucleosome Core Allostery Governs Chromatin Recognition and Cell Fate"],"prefix":"10.64898","author":[{"given":"AN","family":"Wilkening","sequence":"first","affiliation":[{"name":"Department of Genetics, Stanford University; Stanford, USA"},{"name":"Department of Developmental Biology, Stanford University; Stanford, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Priyadarshini","sequence":"additional","affiliation":[{"name":"Department of Genetics, Stanford University; Stanford, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MJ","family":"Trnka","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry, University of California San Francisco; San Francisco, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MMK","family":"Wong","sequence":"additional","affiliation":[{"name":"Department of Genetics, Stanford University; Stanford, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Nawar","sequence":"additional","affiliation":[{"name":"Department of Genetics, Stanford University; Stanford, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Medwid","sequence":"additional","affiliation":[{"name":"Department of Genetics, Stanford University; Stanford, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"DN","family":"Kahan","sequence":"additional","affiliation":[{"name":"Biophysics Graduate Group, University of California, Berkeley; Berkeley, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Marqusee","sequence":"additional","affiliation":[{"name":"Department of Molecular & Cell Biology, University of California, Berkeley; Berkeley, USA"},{"name":"Department of Chemistry, University of California, Berkeley; Berkeley, USA"},{"name":"Chan Zuckerberg Biohub; San Francisco, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Sanulli","sequence":"additional","affiliation":[{"name":"Department of Genetics, Stanford University; Stanford, USA"},{"name":"Chan Zuckerberg Biohub; San Francisco, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026093011301070000_2026.09.24.753912v1.1","doi-asserted-by":"publisher","DOI":"10.1021\/cr500373h"},{"key":"2026093011301070000_2026.09.24.753912v1.2","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2011.22"},{"key":"2026093011301070000_2026.09.24.753912v1.3","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.1454"},{"key":"2026093011301070000_2026.09.24.753912v1.4","doi-asserted-by":"publisher","DOI":"10.1038\/s41589-021-00738-1"},{"key":"2026093011301070000_2026.09.24.753912v1.5","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-1038-1"},{"key":"2026093011301070000_2026.09.24.753912v1.6","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abc9207"},{"key":"2026093011301070000_2026.09.24.753912v1.7","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2023.02.023"},{"key":"2026093011301070000_2026.09.24.753912v1.8","doi-asserted-by":"publisher","DOI":"10.1038\/nrm.2016.166"},{"key":"2026093011301070000_2026.09.24.753912v1.9","doi-asserted-by":"publisher","DOI":"10.1038\/s41576-024-00759-1"},{"key":"2026093011301070000_2026.09.24.753912v1.10","doi-asserted-by":"publisher","DOI":"10.1038\/nrg3673"},{"key":"2026093011301070000_2026.09.24.753912v1.11","doi-asserted-by":"publisher","DOI":"10.1186\/1741-7007-7-86"},{"key":"2026093011301070000_2026.09.24.753912v1.12","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.08.002"},{"key":"2026093011301070000_2026.09.24.753912v1.13","doi-asserted-by":"publisher","DOI":"10.1107\/S090744491302252X"},{"key":"2026093011301070000_2026.09.24.753912v1.14","doi-asserted-by":"publisher","DOI":"10.1523\/ENEURO.0040-17.2017"},{"key":"2026093011301070000_2026.09.24.753912v1.15","doi-asserted-by":"publisher","DOI":"10.1186\/s13072-019-0274-9"},{"key":"2026093011301070000_2026.09.24.753912v1.16","doi-asserted-by":"publisher","DOI":"10.1139\/o06-077"},{"key":"2026093011301070000_2026.09.24.753912v1.17","doi-asserted-by":"publisher","DOI":"10.1038\/81971"},{"key":"2026093011301070000_2026.09.24.753912v1.18","doi-asserted-by":"publisher","DOI":"10.1038\/nature12242"},{"key":"2026093011301070000_2026.09.24.753912v1.19","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2013.07.015"},{"key":"2026093011301070000_2026.09.24.753912v1.20","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcpro.2026.101518"},{"key":"2026093011301070000_2026.09.24.753912v1.21","doi-asserted-by":"publisher","DOI":"10.1016\/j.fob.2013.08.007"},{"key":"2026093011301070000_2026.09.24.753912v1.22","doi-asserted-by":"publisher","DOI":"10.1038\/srep31437"},{"key":"2026093011301070000_2026.09.24.753912v1.23","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-36465-5"},{"key":"2026093011301070000_2026.09.24.753912v1.24","doi-asserted-by":"publisher","DOI":"10.1186\/1741-7007-7-86"},{"key":"2026093011301070000_2026.09.24.753912v1.25","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2148-9-31"},{"key":"2026093011301070000_2026.09.24.753912v1.26","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2015.05.009"},{"key":"2026093011301070000_2026.09.24.753912v1.27","doi-asserted-by":"publisher","DOI":"10.1080\/23723556.2015.1073417"},{"key":"2026093011301070000_2026.09.24.753912v1.28","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-1669-2"},{"key":"2026093011301070000_2026.09.24.753912v1.29","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.08.037"},{"key":"2026093011301070000_2026.09.24.753912v1.30","doi-asserted-by":"publisher","DOI":"10.1021\/bi060043d"},{"key":"2026093011301070000_2026.09.24.753912v1.31","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2014.30"},{"key":"2026093011301070000_2026.09.24.753912v1.32","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms15616"},{"key":"2026093011301070000_2026.09.24.753912v1.33","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.53375"},{"key":"2026093011301070000_2026.09.24.753912v1.34","doi-asserted-by":"publisher","DOI":"10.15252\/embj.201695757"},{"key":"2026093011301070000_2026.09.24.753912v1.35","doi-asserted-by":"publisher","DOI":"10.1515\/BC-2011-216"},{"key":"2026093011301070000_2026.09.24.753912v1.36","doi-asserted-by":"publisher","DOI":"10.1126\/science.1090701"},{"key":"2026093011301070000_2026.09.24.753912v1.37","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.09.056"},{"key":"2026093011301070000_2026.09.24.753912v1.38","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2013.01.038"},{"key":"2026093011301070000_2026.09.24.753912v1.39","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2012-13-10-r85"},{"key":"2026093011301070000_2026.09.24.753912v1.40","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2022.04.014"},{"key":"2026093011301070000_2026.09.24.753912v1.41","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2012.11.003"},{"key":"2026093011301070000_2026.09.24.753912v1.42","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(01)00329-3"},{"key":"2026093011301070000_2026.09.24.753912v1.43","doi-asserted-by":"publisher","DOI":"10.1016\/j.tig.2021.10.003"},{"key":"2026093011301070000_2026.09.24.753912v1.44","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx167"},{"key":"2026093011301070000_2026.09.24.753912v1.45","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.11.018"},{"key":"2026093011301070000_2026.09.24.753912v1.46","doi-asserted-by":"publisher","DOI":"10.1038\/ng.409"},{"key":"2026093011301070000_2026.09.24.753912v1.47","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-36465-5"},{"key":"2026093011301070000_2026.09.24.753912v1.48","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biochem.7b00747"},{"key":"2026093011301070000_2026.09.24.753912v1.49","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaa3761"},{"key":"2026093011301070000_2026.09.24.753912v1.50","doi-asserted-by":"publisher","DOI":"10.1038\/s41594-017-0005-5"},{"key":"2026093011301070000_2026.09.24.753912v1.51","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-03677-z"},{"key":"2026093011301070000_2026.09.24.753912v1.52","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.46057"},{"key":"2026093011301070000_2026.09.24.753912v1.53","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(03)75002-2"},{"key":"2026093011301070000_2026.09.24.753912v1.54","doi-asserted-by":"publisher","DOI":"10.1038\/s41594-022-00735-5"},{"key":"2026093011301070000_2026.09.24.753912v1.55","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2014.12.020"},{"key":"2026093011301070000_2026.09.24.753912v1.56","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts635"},{"key":"2026093011301070000_2026.09.24.753912v1.57","doi-asserted-by":"publisher","DOI":"10.1093\/gigascience\/giab008"},{"key":"2026093011301070000_2026.09.24.753912v1.58","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz114"},{"key":"2026093011301070000_2026.09.24.753912v1.59","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8"},{"key":"2026093011301070000_2026.09.24.753912v1.60","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2025.116494"},{"key":"2026093011301070000_2026.09.24.753912v1.61","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M111.010728"},{"key":"2026093011301070000_2026.09.24.753912v1.62","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.D500002-MCP200"},{"key":"2026093011301070000_2026.09.24.753912v1.63","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcpro.2025.100999"},{"key":"2026093011301070000_2026.09.24.753912v1.64","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac1000"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.64898\/2026.09.24.753912","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T18:30:23Z","timestamp":1790793023000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.64898\/2026.09.24.753912"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,28]]},"references-count":64,"URL":"https:\/\/doi.org\/10.64898\/2026.09.24.753912","relation":{},"subject":[],"published":{"date-parts":[[2026,9,28]]},"subtype":"preprint"}}